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Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides

Grey, Carl LU ; Edebrink, Per ; Krook, Maria and Jacobsson, Sven P. (2009) In Journal of Chromatography. B 877(20-21). p.1827-1832
Abstract
In the present study a HPAEC-PAD method is described that was developed for monitoring the consistency of N-glycosylation during the production and purification of recombinant proteins and monoclonal antibodies. The method successfully separated 18 neutral and sialylated oligosaccharides. Results obtained were compared with MALDI-TOF MS and it was shown that both methods gave similar results. in addition, a method validation was performed showing that the HPAEC-PAD analysis was well suited for the mapping and characterization of oligosaccharides. The method was found to be robust and additionally the precision was significantly better compared to the MALDI-TOF MS method. (C) 2009 Elsevier B.V. All rights reserved.
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Monoclonal antibodies, Glycoprotein, N-glycosylation, Oligosaccharides, HPAEC-PAD, MALDI-TOF MS
in
Journal of Chromatography. B
volume
877
issue
20-21
pages
1827 - 1832
publisher
Elsevier
external identifiers
  • wos:000267453800006
  • scopus:67249087592
  • pmid:19482525
ISSN
1873-376X
DOI
10.1016/j.jchromb.2009.05.003
language
English
LU publication?
yes
id
73a71c15-d4bf-4762-af0e-d1f7dc18874e (old id 1463105)
date added to LUP
2016-04-01 12:07:33
date last changed
2022-04-29 01:01:35
@article{73a71c15-d4bf-4762-af0e-d1f7dc18874e,
  abstract     = {{In the present study a HPAEC-PAD method is described that was developed for monitoring the consistency of N-glycosylation during the production and purification of recombinant proteins and monoclonal antibodies. The method successfully separated 18 neutral and sialylated oligosaccharides. Results obtained were compared with MALDI-TOF MS and it was shown that both methods gave similar results. in addition, a method validation was performed showing that the HPAEC-PAD analysis was well suited for the mapping and characterization of oligosaccharides. The method was found to be robust and additionally the precision was significantly better compared to the MALDI-TOF MS method. (C) 2009 Elsevier B.V. All rights reserved.}},
  author       = {{Grey, Carl and Edebrink, Per and Krook, Maria and Jacobsson, Sven P.}},
  issn         = {{1873-376X}},
  keywords     = {{Monoclonal antibodies; Glycoprotein; N-glycosylation; Oligosaccharides; HPAEC-PAD; MALDI-TOF MS}},
  language     = {{eng}},
  number       = {{20-21}},
  pages        = {{1827--1832}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Chromatography. B}},
  title        = {{Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides}},
  url          = {{http://dx.doi.org/10.1016/j.jchromb.2009.05.003}},
  doi          = {{10.1016/j.jchromb.2009.05.003}},
  volume       = {{877}},
  year         = {{2009}},
}