Phosphorus effects on metabolic processes in monoxenic arbuscular mycorrhiza cultures
(2002) In Plant Physiology 130(3). p.1162-1171- Abstract
- The influence of external phosphorus (P) on carbon (C) allocation and metabolism as well as processes related to P metabolism was studied in monoxenic arbuscular mycorrhiza cultures of carrot (Daucus carota). Fungal hyphae of Glomus intraradices proliferated from the solid minimal medium containing the colonized roots into C-free liquid minimal medium with different P treatments. The fungus formed around three times higher biomass in P-free liquid medium than in medium with 2.5 mM inorganic P (high-P). Mycelium in the second experiment was harvested at an earlier growth stag to study metabolic processes when the mycelium was actively growing. P treatment influenced the root P content and [C-13]glucose administered to the roots 7 d before... (More)
- The influence of external phosphorus (P) on carbon (C) allocation and metabolism as well as processes related to P metabolism was studied in monoxenic arbuscular mycorrhiza cultures of carrot (Daucus carota). Fungal hyphae of Glomus intraradices proliferated from the solid minimal medium containing the colonized roots into C-free liquid minimal medium with different P treatments. The fungus formed around three times higher biomass in P-free liquid medium than in medium with 2.5 mM inorganic P (high-P). Mycelium in the second experiment was harvested at an earlier growth stag to study metabolic processes when the mycelium was actively growing. P treatment influenced the root P content and [C-13]glucose administered to the roots 7 d before harvest gave a negative correlation between root P content and C-13 enrichment in arbuscular mycorrhiza fungal storage lipids in the extraradical hyphae. Eighteen percent of the enriched C-13 in extraradical hyphae was recovered in the fatty acid 16:1omega5 from neutral lipids. Polyphosphate accumulated in hyphae even in P-free medium. No influence of P treatment on fungal acid phosphatase activity was observed, whereas the proportion of alkaline-phosphatase-active hyphae was highest in high-P medium. We demonstrated the presence of a motile tubular vacuolar system in G. intraradices. This system was rarely seen in hyphae subjected to the highest P treatment. We concluded that the direct responses of the extraradical hyphae to the P concentration in the medium are limited. The effects found in hyphae seemed instead to be related to increased availability of P to the host root. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/146885
- author
- Olsson, Pål Axel LU ; van Aarle, Ingrid LU ; Allaway, W G ; Ashford, A E and Rouhier, Hervé LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Plant Physiology
- volume
- 130
- issue
- 3
- pages
- 1162 - 1171
- publisher
- American Society of Plant Biologists
- external identifiers
-
- pmid:12427983
- wos:000179329400010
- scopus:0036852160
- pmid:12427983
- ISSN
- 1532-2548
- DOI
- 10.1104/pp.009639
- language
- English
- LU publication?
- yes
- id
- e1a650cb-1e3f-4b53-80ba-374ae19bda50 (old id 146885)
- date added to LUP
- 2016-04-01 12:24:37
- date last changed
- 2022-01-27 03:21:55
@article{e1a650cb-1e3f-4b53-80ba-374ae19bda50,
abstract = {{The influence of external phosphorus (P) on carbon (C) allocation and metabolism as well as processes related to P metabolism was studied in monoxenic arbuscular mycorrhiza cultures of carrot (Daucus carota). Fungal hyphae of Glomus intraradices proliferated from the solid minimal medium containing the colonized roots into C-free liquid minimal medium with different P treatments. The fungus formed around three times higher biomass in P-free liquid medium than in medium with 2.5 mM inorganic P (high-P). Mycelium in the second experiment was harvested at an earlier growth stag to study metabolic processes when the mycelium was actively growing. P treatment influenced the root P content and [C-13]glucose administered to the roots 7 d before harvest gave a negative correlation between root P content and C-13 enrichment in arbuscular mycorrhiza fungal storage lipids in the extraradical hyphae. Eighteen percent of the enriched C-13 in extraradical hyphae was recovered in the fatty acid 16:1omega5 from neutral lipids. Polyphosphate accumulated in hyphae even in P-free medium. No influence of P treatment on fungal acid phosphatase activity was observed, whereas the proportion of alkaline-phosphatase-active hyphae was highest in high-P medium. We demonstrated the presence of a motile tubular vacuolar system in G. intraradices. This system was rarely seen in hyphae subjected to the highest P treatment. We concluded that the direct responses of the extraradical hyphae to the P concentration in the medium are limited. The effects found in hyphae seemed instead to be related to increased availability of P to the host root.}},
author = {{Olsson, Pål Axel and van Aarle, Ingrid and Allaway, W G and Ashford, A E and Rouhier, Hervé}},
issn = {{1532-2548}},
language = {{eng}},
number = {{3}},
pages = {{1162--1171}},
publisher = {{American Society of Plant Biologists}},
series = {{Plant Physiology}},
title = {{Phosphorus effects on metabolic processes in monoxenic arbuscular mycorrhiza cultures}},
url = {{http://dx.doi.org/10.1104/pp.009639}},
doi = {{10.1104/pp.009639}},
volume = {{130}},
year = {{2002}},
}