Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium
(2005) In Applied Microbiology and Biotechnology 67(5). p.684-691- Abstract
- Supplementation of a chemically defined medium with amino acids or succinate to improve heterologous xylanase production by a prototrophic Saccharomyces cerevisiae transformant was investigated. The corresponding xylanase production during growth on ethanol in batch culture and in glucose-limited chemostat culture were quantified, as the native ADH2 promoter regulating xylanase expression was derepressed under these conditions. The addition of a balanced mixture of the preferred amino acids, Ala, Arg, Asn, Glu, Gln and Gly, improved both biomass and xylanase production, whereas several other individual amino acids inhibited biomass and/or xylanase production. Heterologous protein production by the recombinant yeast was also improved by... (More)
- Supplementation of a chemically defined medium with amino acids or succinate to improve heterologous xylanase production by a prototrophic Saccharomyces cerevisiae transformant was investigated. The corresponding xylanase production during growth on ethanol in batch culture and in glucose-limited chemostat culture were quantified, as the native ADH2 promoter regulating xylanase expression was derepressed under these conditions. The addition of a balanced mixture of the preferred amino acids, Ala, Arg, Asn, Glu, Gln and Gly, improved both biomass and xylanase production, whereas several other individual amino acids inhibited biomass and/or xylanase production. Heterologous protein production by the recombinant yeast was also improved by supplementing the medium with succinate. The production of heterologous xylanase during growth on ethanol or glucose could thus be improved by supplementing metabolic precursors in the carbon- or nitrogen-metabolism. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/151244
- author
- Görgens, Johann F ; van Zyl, WH ; Knoetze, JH and Hahn-Hägerdal, Bärbel LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied Microbiology and Biotechnology
- volume
- 67
- issue
- 5
- pages
- 684 - 691
- publisher
- Springer
- external identifiers
-
- wos:000229722400014
- pmid:15630584
- scopus:21244466810
- pmid:15630584
- ISSN
- 1432-0614
- DOI
- 10.1007/s00253-004-1803-3
- language
- English
- LU publication?
- yes
- id
- 7c56cb4b-be29-4ca8-bfce-4a95a89f95f2 (old id 151244)
- date added to LUP
- 2016-04-01 16:50:39
- date last changed
- 2022-01-28 22:33:59
@article{7c56cb4b-be29-4ca8-bfce-4a95a89f95f2,
abstract = {{Supplementation of a chemically defined medium with amino acids or succinate to improve heterologous xylanase production by a prototrophic Saccharomyces cerevisiae transformant was investigated. The corresponding xylanase production during growth on ethanol in batch culture and in glucose-limited chemostat culture were quantified, as the native ADH2 promoter regulating xylanase expression was derepressed under these conditions. The addition of a balanced mixture of the preferred amino acids, Ala, Arg, Asn, Glu, Gln and Gly, improved both biomass and xylanase production, whereas several other individual amino acids inhibited biomass and/or xylanase production. Heterologous protein production by the recombinant yeast was also improved by supplementing the medium with succinate. The production of heterologous xylanase during growth on ethanol or glucose could thus be improved by supplementing metabolic precursors in the carbon- or nitrogen-metabolism.}},
author = {{Görgens, Johann F and van Zyl, WH and Knoetze, JH and Hahn-Hägerdal, Bärbel}},
issn = {{1432-0614}},
language = {{eng}},
number = {{5}},
pages = {{684--691}},
publisher = {{Springer}},
series = {{Applied Microbiology and Biotechnology}},
title = {{Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium}},
url = {{http://dx.doi.org/10.1007/s00253-004-1803-3}},
doi = {{10.1007/s00253-004-1803-3}},
volume = {{67}},
year = {{2005}},
}