Improved matrix-assisted laser desorption/ionisation sample preparation of a partially depolymerised cellulose derivative by continuous spray deposition and interfacing with size-exclusion chromatography
(2005) In Rapid Communications in Mass Spectrometry 19(7). p.947-954- Abstract
- Continuous spray deposition (CSD) of aqueous solutions of partially depolymerised methyl cellulose was found to improve matrix-assisted laser desorption/ionisation (MALDI) sample preparation. One feature was that the sensitivity in MALDI time-of-flight mass spectrometry increased up to an order of magnitude compared with the standard sample preparation method. Another feature was that CSD provided targets for MALDI with homogeneously distributed analyte. This resulted in a more even signal intensity and a higher reproducibility than in the standard method. High-mass discrimination was more pronounced in CSD than in the standard method. Size-exclusion chromatography with aqueous eluent was coupled online to CSD onto matrix-precoated foils.... (More)
- Continuous spray deposition (CSD) of aqueous solutions of partially depolymerised methyl cellulose was found to improve matrix-assisted laser desorption/ionisation (MALDI) sample preparation. One feature was that the sensitivity in MALDI time-of-flight mass spectrometry increased up to an order of magnitude compared with the standard sample preparation method. Another feature was that CSD provided targets for MALDI with homogeneously distributed analyte. This resulted in a more even signal intensity and a higher reproducibility than in the standard method. High-mass discrimination was more pronounced in CSD than in the standard method. Size-exclusion chromatography with aqueous eluent was coupled online to CSD onto matrix-precoated foils. The suitability for determination of the molar mass distribution of methyl cellulose was investigated. Copyright (c) 2005 John Wiley C Sons, Ltd. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/152667
- author
- Momcilovic, Dane LU ; Wahlund, Karl-Gustav LU ; Wittgren, B and Brinkmalm, G
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Rapid Communications in Mass Spectrometry
- volume
- 19
- issue
- 7
- pages
- 947 - 954
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:15747325
- wos:000227907600012
- scopus:15944410672
- ISSN
- 1097-0231
- DOI
- 10.1002/rcm.1874
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- d43bad9f-f83f-4d6a-9bfe-9effa92f3fc4 (old id 152667)
- date added to LUP
- 2016-04-01 11:49:30
- date last changed
- 2022-04-20 22:19:52
@article{d43bad9f-f83f-4d6a-9bfe-9effa92f3fc4, abstract = {{Continuous spray deposition (CSD) of aqueous solutions of partially depolymerised methyl cellulose was found to improve matrix-assisted laser desorption/ionisation (MALDI) sample preparation. One feature was that the sensitivity in MALDI time-of-flight mass spectrometry increased up to an order of magnitude compared with the standard sample preparation method. Another feature was that CSD provided targets for MALDI with homogeneously distributed analyte. This resulted in a more even signal intensity and a higher reproducibility than in the standard method. High-mass discrimination was more pronounced in CSD than in the standard method. Size-exclusion chromatography with aqueous eluent was coupled online to CSD onto matrix-precoated foils. The suitability for determination of the molar mass distribution of methyl cellulose was investigated. Copyright (c) 2005 John Wiley C Sons, Ltd.}}, author = {{Momcilovic, Dane and Wahlund, Karl-Gustav and Wittgren, B and Brinkmalm, G}}, issn = {{1097-0231}}, language = {{eng}}, number = {{7}}, pages = {{947--954}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Rapid Communications in Mass Spectrometry}}, title = {{Improved matrix-assisted laser desorption/ionisation sample preparation of a partially depolymerised cellulose derivative by continuous spray deposition and interfacing with size-exclusion chromatography}}, url = {{http://dx.doi.org/10.1002/rcm.1874}}, doi = {{10.1002/rcm.1874}}, volume = {{19}}, year = {{2005}}, }