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Differential Effects of Ferulic Acid and p-Coumaric Acid on S Phase Distribution and Length of S Phase in the Human Colonic Cell Line Caco-2

Janicke, Birgit LU ; Önning, Gunilla LU and Oredsson, S M (2005) In Journal of Agricultural and Food Chemistry 53(17). p.6658-6665
Abstract
Ferulic acid (FA) and para-coumaric acid (p-CA) may mediate the protective effects of whole-grain cereals against colon cancer. Therefore, the effects of FA and p-CA on the metabolic activity, proliferation, cell cycle phase distribution, and kinetics of the colonic endothelial tumor cell line Caco-2 was studied. Both compounds at 1500 M decreased the number of cells to 43-75% of control after 2-3 days of treatment. Cell cycle phase distribution and cell cycle kinetics were determined by flow cytometric analysis after bromodeoxyuridine labeling. Each compound at 1500 M decreased the proportion of cells in the G1 phase and increased the proportion of cells in the S and G2 phases. Treatment with 1500 M FA significantly increased the length... (More)
Ferulic acid (FA) and para-coumaric acid (p-CA) may mediate the protective effects of whole-grain cereals against colon cancer. Therefore, the effects of FA and p-CA on the metabolic activity, proliferation, cell cycle phase distribution, and kinetics of the colonic endothelial tumor cell line Caco-2 was studied. Both compounds at 1500 M decreased the number of cells to 43-75% of control after 2-3 days of treatment. Cell cycle phase distribution and cell cycle kinetics were determined by flow cytometric analysis after bromodeoxyuridine labeling. Each compound at 1500 M decreased the proportion of cells in the G1 phase and increased the proportion of cells in the S and G2 phases. Treatment with 1500 M FA significantly increased the length of the S phase, while p-CA did not. It was concluded that FA and p-CA inhibited cell proliferation by presumably affecting different cell cycle phases, and this warrants further investigations because this inhibition may be one explanation for the diet-related protection against cancer. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
p-coumaric acid, Cell cycle, proliferation, cereals, ferulic acid
in
Journal of Agricultural and Food Chemistry
volume
53
issue
17
pages
6658 - 6665
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:16104781
  • wos:000231459900015
  • scopus:24344464239
  • pmid:16104781
ISSN
0021-8561
DOI
10.1021/jf050489l
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pure and Applied Biochemistry (LTH) (011001005), Animal Physiology (Closed 2011) (011011000), Center for Chemistry and Chemical Engineering (011001000)
id
b4b7ef2f-f837-4f3c-82d3-3176515208f6 (old id 153233)
date added to LUP
2016-04-01 11:46:45
date last changed
2022-03-20 18:49:55
@article{b4b7ef2f-f837-4f3c-82d3-3176515208f6,
  abstract     = {{Ferulic acid (FA) and para-coumaric acid (p-CA) may mediate the protective effects of whole-grain cereals against colon cancer. Therefore, the effects of FA and p-CA on the metabolic activity, proliferation, cell cycle phase distribution, and kinetics of the colonic endothelial tumor cell line Caco-2 was studied. Both compounds at 1500 M decreased the number of cells to 43-75% of control after 2-3 days of treatment. Cell cycle phase distribution and cell cycle kinetics were determined by flow cytometric analysis after bromodeoxyuridine labeling. Each compound at 1500 M decreased the proportion of cells in the G1 phase and increased the proportion of cells in the S and G2 phases. Treatment with 1500 M FA significantly increased the length of the S phase, while p-CA did not. It was concluded that FA and p-CA inhibited cell proliferation by presumably affecting different cell cycle phases, and this warrants further investigations because this inhibition may be one explanation for the diet-related protection against cancer.}},
  author       = {{Janicke, Birgit and Önning, Gunilla and Oredsson, S M}},
  issn         = {{0021-8561}},
  keywords     = {{p-coumaric acid; Cell cycle; proliferation; cereals; ferulic acid}},
  language     = {{eng}},
  number       = {{17}},
  pages        = {{6658--6665}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Journal of Agricultural and Food Chemistry}},
  title        = {{Differential Effects of Ferulic Acid and p-Coumaric Acid on S Phase Distribution and Length of S Phase in the Human Colonic Cell Line Caco-2}},
  url          = {{http://dx.doi.org/10.1021/jf050489l}},
  doi          = {{10.1021/jf050489l}},
  volume       = {{53}},
  year         = {{2005}},
}