Quantification of Membrane Proteins Using Nonspecific Protease Digestions
(2009) In Journal of Proteome Research 8(12). p.5666-5673- Abstract
- We present a mass spectrometry-based method for the identification and quantification of membrane proteins using the low-specificity protease Proteinase K, at very high pH, to digest proteins isolated by a modified SDS-PAGE protocol. The resulting peptides are modified with a fragmentation-directing isotope labeled tag. We apply the method to quantify differences in membrane protein expression of Bacillus subtilis grown in the presence or absence of glucose.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1533438
- author
- Bendz, Maria ; Carlsson Möller, Mirja LU ; Arrigoni, Giorgio LU ; Wåhlander, Åsa LU ; Stella, Roberto ; Cappadona, Salvatore LU ; Levander, Fredrik LU ; Hederstedt, Lars LU and James, Peter LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- membrane proteins, N-terminal labeling, quantification, relative, proteinase K, Bacillus subtilis
- in
- Journal of Proteome Research
- volume
- 8
- issue
- 12
- pages
- 5666 - 5673
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000272339100026
- scopus:71549132222
- ISSN
- 1535-3893
- DOI
- 10.1021/pr900741t
- language
- English
- LU publication?
- yes
- id
- b5889499-14d3-4b76-b0a7-097924af96a8 (old id 1533438)
- date added to LUP
- 2016-04-01 12:38:08
- date last changed
- 2024-01-09 03:28:02
@article{b5889499-14d3-4b76-b0a7-097924af96a8, abstract = {{We present a mass spectrometry-based method for the identification and quantification of membrane proteins using the low-specificity protease Proteinase K, at very high pH, to digest proteins isolated by a modified SDS-PAGE protocol. The resulting peptides are modified with a fragmentation-directing isotope labeled tag. We apply the method to quantify differences in membrane protein expression of Bacillus subtilis grown in the presence or absence of glucose.}}, author = {{Bendz, Maria and Carlsson Möller, Mirja and Arrigoni, Giorgio and Wåhlander, Åsa and Stella, Roberto and Cappadona, Salvatore and Levander, Fredrik and Hederstedt, Lars and James, Peter}}, issn = {{1535-3893}}, keywords = {{membrane proteins; N-terminal labeling; quantification; relative; proteinase K; Bacillus subtilis}}, language = {{eng}}, number = {{12}}, pages = {{5666--5673}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Journal of Proteome Research}}, title = {{Quantification of Membrane Proteins Using Nonspecific Protease Digestions}}, url = {{http://dx.doi.org/10.1021/pr900741t}}, doi = {{10.1021/pr900741t}}, volume = {{8}}, year = {{2009}}, }