Effects of Carbon Dioxide on Growth of Proteolytic Clostridium botulinum, Its Ability To Produce Neurotoxin, and Its Transcriptome
(2010) In Applied and Environmental Microbiology 76(4). p.1168-1172- Abstract
- The antimicrobial gas carbon dioxide is frequently used in modified atmosphere packaging. In the present study, the effects of CO2 (10 to 70%, vol/vol) on gene expression (measured using quantitative reverse transcription-PCR and a whole-genome DNA microarray) and neurotoxin formation (measured using an enzyme-linked immunosorbent assay [ELISA]) by proteolytic Clostridium botulinum type A1 strain ATCC 3502 were studied during the growth cycle. Interestingly, in marked contrast to the situation with nonproteolytic C. botulinum types B and E, CO2 had little effect on any of these parameters. At all CO2 concentrations, relative expression of neurotoxin cluster genes peaked in the transition between exponential and stationary phases, with... (More)
- The antimicrobial gas carbon dioxide is frequently used in modified atmosphere packaging. In the present study, the effects of CO2 (10 to 70%, vol/vol) on gene expression (measured using quantitative reverse transcription-PCR and a whole-genome DNA microarray) and neurotoxin formation (measured using an enzyme-linked immunosorbent assay [ELISA]) by proteolytic Clostridium botulinum type A1 strain ATCC 3502 were studied during the growth cycle. Interestingly, in marked contrast to the situation with nonproteolytic C. botulinum types B and E, CO2 had little effect on any of these parameters. At all CO2 concentrations, relative expression of neurotoxin cluster genes peaked in the transition between exponential and stationary phases, with evidence of a second rise in expression in late stationary phase. Microarray analysis enabled identification of coding sequences whose expression profiles matched those of the neurotoxin cluster. Further research is needed to determine whether these are connected to neurotoxin formation or are merely growth phase associated. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1571218
- author
- Artin, Ingrid LU ; Mason, David R. ; Pin, Carmen ; Schelin, Jenny LU ; Peck, Michael W. ; Holst, Elisabet LU ; Rådström, Peter LU and Carter, Andrew T.
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied and Environmental Microbiology
- volume
- 76
- issue
- 4
- pages
- 1168 - 1172
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000274328900024
- scopus:76649125882
- pmid:20038699
- pmid:20038699
- ISSN
- 0099-2240
- DOI
- 10.1128/AEM.02247-09
- language
- English
- LU publication?
- yes
- id
- bfc39259-c1ce-486e-9e4d-9f681e66138f (old id 1571218)
- date added to LUP
- 2016-04-01 10:49:36
- date last changed
- 2022-03-27 19:52:55
@article{bfc39259-c1ce-486e-9e4d-9f681e66138f, abstract = {{The antimicrobial gas carbon dioxide is frequently used in modified atmosphere packaging. In the present study, the effects of CO2 (10 to 70%, vol/vol) on gene expression (measured using quantitative reverse transcription-PCR and a whole-genome DNA microarray) and neurotoxin formation (measured using an enzyme-linked immunosorbent assay [ELISA]) by proteolytic Clostridium botulinum type A1 strain ATCC 3502 were studied during the growth cycle. Interestingly, in marked contrast to the situation with nonproteolytic C. botulinum types B and E, CO2 had little effect on any of these parameters. At all CO2 concentrations, relative expression of neurotoxin cluster genes peaked in the transition between exponential and stationary phases, with evidence of a second rise in expression in late stationary phase. Microarray analysis enabled identification of coding sequences whose expression profiles matched those of the neurotoxin cluster. Further research is needed to determine whether these are connected to neurotoxin formation or are merely growth phase associated.}}, author = {{Artin, Ingrid and Mason, David R. and Pin, Carmen and Schelin, Jenny and Peck, Michael W. and Holst, Elisabet and Rådström, Peter and Carter, Andrew T.}}, issn = {{0099-2240}}, language = {{eng}}, number = {{4}}, pages = {{1168--1172}}, publisher = {{American Society for Microbiology}}, series = {{Applied and Environmental Microbiology}}, title = {{Effects of Carbon Dioxide on Growth of Proteolytic Clostridium botulinum, Its Ability To Produce Neurotoxin, and Its Transcriptome}}, url = {{http://dx.doi.org/10.1128/AEM.02247-09}}, doi = {{10.1128/AEM.02247-09}}, volume = {{76}}, year = {{2010}}, }