Capsule typing of haemophilus influenzae by matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry
(2018) In Emerging Infectious Diseases 24(3). p.443-452- Abstract
Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isolates of all capsule types (a–f and nontypeable; n = 258) and isogenic capsule transformants (types a-d) were investigated. Principal component and biomarker analyses of mass spectra showed clustering, and mass peaks correlated with capsule type-specific genetic lineages. We used 31 selected isolates to construct a capsule typing database. Validation with the remaining isolates (n = 227) showed 100% sensitivity and 92.2% specificity for... (More)
Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isolates of all capsule types (a–f and nontypeable; n = 258) and isogenic capsule transformants (types a-d) were investigated. Principal component and biomarker analyses of mass spectra showed clustering, and mass peaks correlated with capsule type-specific genetic lineages. We used 31 selected isolates to construct a capsule typing database. Validation with the remaining isolates (n = 227) showed 100% sensitivity and 92.2% specificity for encapsulated strains (a–f; n = 61). Blinded validation of a supplemented database (n = 50) using clinical isolates (n = 126) showed 100% sensitivity and 100% specificity for encapsulated strains (b, e, and f; n = 28). MALDI-TOF mass spectrometry is an accurate method for capsule typing of H. influenzae.
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- author
- Månsson, Viktor LU ; Gilsdorf, Janet R. ; Kahlmeter, Gunnar ; Kilian, Mogens ; Kroll, J. Simon ; Riesbeck, Kristian LU and Resman, Fredrik LU
- organization
- publishing date
- 2018-03-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Emerging Infectious Diseases
- volume
- 24
- issue
- 3
- pages
- 10 pages
- publisher
- Centers for Disease Control and Prevention
- external identifiers
-
- scopus:85042311159
- pmid:29460728
- ISSN
- 1080-6040
- DOI
- 10.3201/eid2403.170459
- language
- English
- LU publication?
- yes
- id
- 1575105b-c95e-4770-89f2-6ed493c52c93
- date added to LUP
- 2018-03-17 18:57:38
- date last changed
- 2024-07-22 14:11:58
@article{1575105b-c95e-4770-89f2-6ed493c52c93, abstract = {{<p>Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isolates of all capsule types (a–f and nontypeable; n = 258) and isogenic capsule transformants (types a-d) were investigated. Principal component and biomarker analyses of mass spectra showed clustering, and mass peaks correlated with capsule type-specific genetic lineages. We used 31 selected isolates to construct a capsule typing database. Validation with the remaining isolates (n = 227) showed 100% sensitivity and 92.2% specificity for encapsulated strains (a–f; n = 61). Blinded validation of a supplemented database (n = 50) using clinical isolates (n = 126) showed 100% sensitivity and 100% specificity for encapsulated strains (b, e, and f; n = 28). MALDI-TOF mass spectrometry is an accurate method for capsule typing of H. influenzae.</p>}}, author = {{Månsson, Viktor and Gilsdorf, Janet R. and Kahlmeter, Gunnar and Kilian, Mogens and Kroll, J. Simon and Riesbeck, Kristian and Resman, Fredrik}}, issn = {{1080-6040}}, language = {{eng}}, month = {{03}}, number = {{3}}, pages = {{443--452}}, publisher = {{Centers for Disease Control and Prevention}}, series = {{Emerging Infectious Diseases}}, title = {{Capsule typing of haemophilus influenzae by matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry}}, url = {{http://dx.doi.org/10.3201/eid2403.170459}}, doi = {{10.3201/eid2403.170459}}, volume = {{24}}, year = {{2018}}, }