Probing control of glucose feeding in Vibrio cholerae cultivations
(2003) In Bioprocess and Biosystems Engineering 25(4). p.221-228- Abstract
- Infection with Vibrio cholerae is a significant problem in manydeveloping countries. Cultivation of Vibrio cholerae is used inproduction of CTB, which is a component in a cholera vaccine. Fed - batch cultivations with V. cholerae in defined mediahave been conducted and reproducible results were obtained. A probing feeding strategy developed by Åkessonfor E. coli cultivations has been tested.The program is working as well for V. cholerae as forE. coliin minimizing the amount acetic acid formed and avoiding anaerobicconditions. Two hours after the feed start most of the acetic acidaccumulated during the batch phase is consumed. The resulting feedrate tends to be thehighest possible with respect to the constraints from cell metabolismand mass... (More)
- Infection with Vibrio cholerae is a significant problem in manydeveloping countries. Cultivation of Vibrio cholerae is used inproduction of CTB, which is a component in a cholera vaccine. Fed - batch cultivations with V. cholerae in defined mediahave been conducted and reproducible results were obtained. A probing feeding strategy developed by Åkessonfor E. coli cultivations has been tested.The program is working as well for V. cholerae as forE. coliin minimizing the amount acetic acid formed and avoiding anaerobicconditions. Two hours after the feed start most of the acetic acidaccumulated during the batch phase is consumed. The resulting feedrate tends to be thehighest possible with respect to the constraints from cell metabolismand mass transfer, thus maximizing productivity in terms of biomass.A cell dry weight of 20 - 23 g/l is obtained after12 hours of feeding. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/161684
- author
- de Maré, Lena LU ; Andersson, Lena and Hagander, Per LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- fed-batch cultivations, V. cholerae, probing feeding strategy
- in
- Bioprocess and Biosystems Engineering
- volume
- 25
- issue
- 4
- pages
- 221 - 228
- publisher
- Springer
- external identifiers
-
- wos:000180866700004
- scopus:0037253403
- ISSN
- 1615-7605
- DOI
- 10.1007/s00449-002-0304-y
- language
- English
- LU publication?
- yes
- id
- a3ac08a8-1f54-4419-9e76-8bc8f091af95 (old id 161684)
- date added to LUP
- 2016-04-01 16:15:46
- date last changed
- 2022-02-05 07:00:36
@article{a3ac08a8-1f54-4419-9e76-8bc8f091af95,
abstract = {{Infection with Vibrio cholerae is a significant problem in manydeveloping countries. Cultivation of Vibrio cholerae is used inproduction of CTB, which is a component in a cholera vaccine. Fed - batch cultivations with V. cholerae in defined mediahave been conducted and reproducible results were obtained. A probing feeding strategy developed by Åkessonfor E. coli cultivations has been tested.The program is working as well for V. cholerae as forE. coliin minimizing the amount acetic acid formed and avoiding anaerobicconditions. Two hours after the feed start most of the acetic acidaccumulated during the batch phase is consumed. The resulting feedrate tends to be thehighest possible with respect to the constraints from cell metabolismand mass transfer, thus maximizing productivity in terms of biomass.A cell dry weight of 20 - 23 g/l is obtained after12 hours of feeding.}},
author = {{de Maré, Lena and Andersson, Lena and Hagander, Per}},
issn = {{1615-7605}},
keywords = {{fed-batch cultivations; V. cholerae; probing feeding strategy}},
language = {{eng}},
number = {{4}},
pages = {{221--228}},
publisher = {{Springer}},
series = {{Bioprocess and Biosystems Engineering}},
title = {{Probing control of glucose feeding in Vibrio cholerae cultivations}},
url = {{https://lup.lub.lu.se/search/files/4619349/8866321.pdf}},
doi = {{10.1007/s00449-002-0304-y}},
volume = {{25}},
year = {{2003}},
}