Immunoglobulin subclass profiles of anti-idiotypic antibodies to GAD65Ab differ between type 1 diabetes patients and healthy individuals.
(2011) In Scandinavian Journal of Immunology 74. p.363-367- Abstract
- Previously we reported the presence of anti-idiotypic antibodies (anti-Id) specific to autoantibodies against GAD65 (GAD65Ab) in healthy individuals while the activity of anti-Id directed to GAD65Ab in T1D patients was significantly lower. These anti-Id recognize the antigen binding site of GAD65Ab, thus preventing their binding to GAD65. Here we characterized the IgG subclass profile of these anti-Id (GAD65Ab-specific) and of the associated GAD65Ab themselves. The IgG subclass response of anti-Id in healthy individuals (n=16) was IgG3-dominated, while in T1D patients (n=8) IgG1 was the major IgG subclass. The GAD65Ab bound by anti-Id in both healthy individuals (n=38) and GAD65Ab-negative T1D patients (n=35) showed a predominant rank... (More)
- Previously we reported the presence of anti-idiotypic antibodies (anti-Id) specific to autoantibodies against GAD65 (GAD65Ab) in healthy individuals while the activity of anti-Id directed to GAD65Ab in T1D patients was significantly lower. These anti-Id recognize the antigen binding site of GAD65Ab, thus preventing their binding to GAD65. Here we characterized the IgG subclass profile of these anti-Id (GAD65Ab-specific) and of the associated GAD65Ab themselves. The IgG subclass response of anti-Id in healthy individuals (n=16) was IgG3-dominated, while in T1D patients (n=8) IgG1 was the major IgG subclass. The GAD65Ab bound by anti-Id in both healthy individuals (n=38) and GAD65Ab-negative T1D patients (n=35) showed a predominant rank order of IgG1>IgG2>IgG4>IgG3. However, the frequency of GAD65Ab of the IgG4 subclass was significantly higher in T1D patients (p<0.05). We conclude that the IgG subclass profile of anti-Id (GAD65Ab-specific) in healthy individuals differs from that in T1D patients. These differences may provide insights in the development of these antibodies. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1936740
- author
- Oak, Shilpa ; Radtke, Jared ; Törn, Carina LU ; Landin-Olsson, Mona LU and Hampe, Christiane S
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scandinavian Journal of Immunology
- volume
- 74
- pages
- 363 - 367
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000295075700004
- pmid:21517929
- scopus:80052666659
- pmid:21517929
- ISSN
- 1365-3083
- DOI
- 10.1111/j.1365-3083.2011.02565.x
- language
- English
- LU publication?
- yes
- id
- 98740472-8aa1-4943-aa3d-e2da934c0a93 (old id 1936740)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21517929?dopt=Abstract
- date added to LUP
- 2016-04-04 09:39:17
- date last changed
- 2024-01-12 16:33:13
@article{98740472-8aa1-4943-aa3d-e2da934c0a93, abstract = {{Previously we reported the presence of anti-idiotypic antibodies (anti-Id) specific to autoantibodies against GAD65 (GAD65Ab) in healthy individuals while the activity of anti-Id directed to GAD65Ab in T1D patients was significantly lower. These anti-Id recognize the antigen binding site of GAD65Ab, thus preventing their binding to GAD65. Here we characterized the IgG subclass profile of these anti-Id (GAD65Ab-specific) and of the associated GAD65Ab themselves. The IgG subclass response of anti-Id in healthy individuals (n=16) was IgG3-dominated, while in T1D patients (n=8) IgG1 was the major IgG subclass. The GAD65Ab bound by anti-Id in both healthy individuals (n=38) and GAD65Ab-negative T1D patients (n=35) showed a predominant rank order of IgG1>IgG2>IgG4>IgG3. However, the frequency of GAD65Ab of the IgG4 subclass was significantly higher in T1D patients (p<0.05). We conclude that the IgG subclass profile of anti-Id (GAD65Ab-specific) in healthy individuals differs from that in T1D patients. These differences may provide insights in the development of these antibodies.}}, author = {{Oak, Shilpa and Radtke, Jared and Törn, Carina and Landin-Olsson, Mona and Hampe, Christiane S}}, issn = {{1365-3083}}, language = {{eng}}, pages = {{363--367}}, publisher = {{Wiley-Blackwell}}, series = {{Scandinavian Journal of Immunology}}, title = {{Immunoglobulin subclass profiles of anti-idiotypic antibodies to GAD65Ab differ between type 1 diabetes patients and healthy individuals.}}, url = {{http://dx.doi.org/10.1111/j.1365-3083.2011.02565.x}}, doi = {{10.1111/j.1365-3083.2011.02565.x}}, volume = {{74}}, year = {{2011}}, }