Proteomic analysis and discovery using affinity proteomics and mass spectrometry.
Olsson, Niclas LU ; Wingren, Christer LU ; Mattsson, Mikael ; James, Peter LU
; O'Connell, David
; Nilsson, Fredrik
; Cahill, Dolores J
and Borrebaeck, Carl
LU
(2011)
In Molecular & Cellular Proteomics
10.
- Abstract
- Antibody-based microarrays are a rapidly evolving affinity-proteomic methodology that recently has shown great promise in clinical applications. The resolution of these proteomic analyses is, however, directly related to the number of data-points, i.e. antibodies, included on the array. Currently, this is a key bottleneck due to limited availability of numerous highly-characterized antibodies. Here, we present a conceptually new method, denoted global proteome survey, opening up the possibility to probe any proteome in a species independent manner while still using a limited set of antibodies. We use context-independent-motif-specific antibodies directed against short amino acid motifs, where each motif is present in up to a few hundred... (More)
- Antibody-based microarrays are a rapidly evolving affinity-proteomic methodology that recently has shown great promise in clinical applications. The resolution of these proteomic analyses is, however, directly related to the number of data-points, i.e. antibodies, included on the array. Currently, this is a key bottleneck due to limited availability of numerous highly-characterized antibodies. Here, we present a conceptually new method, denoted global proteome survey, opening up the possibility to probe any proteome in a species independent manner while still using a limited set of antibodies. We use context-independent-motif-specific antibodies directed against short amino acid motifs, where each motif is present in up to a few hundred different proteins. First, the digested proteome is exposed to these antibodies, whereby motif-containing peptides are enriched, which then are detected and identified by mass spectrometry. In this study, we profiled extracts from human colon tissue, yeast cells lysate, and mouse liver tissue to demonstrate proof-of-concept. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2008063
- author
- Olsson, Niclas
LU
; Wingren, Christer
LU
; Mattsson, Mikael
; James, Peter
LU
; O'Connell, David
; Nilsson, Fredrik
; Cahill, Dolores J
and Borrebaeck, Carl
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular & Cellular Proteomics
- volume
- 10
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- wos:000295773800027
- pmid:21673276
- scopus:80054021069
- ISSN
- 1535-9484
- DOI
- 10.1074/mcp.M110.003962
- language
- English
- LU publication?
- yes
- id
- eafef29c-9e6a-4c51-a657-0d684dcee9a2 (old id 2008063)
- date added to LUP
- 2016-04-01 10:29:58
- date last changed
- 2023-10-26 12:10:15
@article{eafef29c-9e6a-4c51-a657-0d684dcee9a2,
abstract = {{Antibody-based microarrays are a rapidly evolving affinity-proteomic methodology that recently has shown great promise in clinical applications. The resolution of these proteomic analyses is, however, directly related to the number of data-points, i.e. antibodies, included on the array. Currently, this is a key bottleneck due to limited availability of numerous highly-characterized antibodies. Here, we present a conceptually new method, denoted global proteome survey, opening up the possibility to probe any proteome in a species independent manner while still using a limited set of antibodies. We use context-independent-motif-specific antibodies directed against short amino acid motifs, where each motif is present in up to a few hundred different proteins. First, the digested proteome is exposed to these antibodies, whereby motif-containing peptides are enriched, which then are detected and identified by mass spectrometry. In this study, we profiled extracts from human colon tissue, yeast cells lysate, and mouse liver tissue to demonstrate proof-of-concept.}},
author = {{Olsson, Niclas and Wingren, Christer and Mattsson, Mikael and James, Peter and O'Connell, David and Nilsson, Fredrik and Cahill, Dolores J and Borrebaeck, Carl}},
issn = {{1535-9484}},
language = {{eng}},
publisher = {{American Society for Biochemistry and Molecular Biology}},
series = {{Molecular & Cellular Proteomics}},
title = {{Proteomic analysis and discovery using affinity proteomics and mass spectrometry.}},
url = {{http://dx.doi.org/10.1074/mcp.M110.003962}},
doi = {{10.1074/mcp.M110.003962}},
volume = {{10}},
year = {{2011}},
}