Terminal fatty-acyl-CoA desaturase involved in sex pheromone biosynthesis in the Winter Moth (Operophtera brumata)
Ding, Bao-Jian LU ; Liénard, Marjorie LU
; Wang, Hong-Lei
LU
; Zhao, Cheng-Hua
LU
and Löfstedt, Christer
LU
(2011)
In Insect Biochemistry and Molecular Biology
41.
p.715-722
- Abstract
- The Winter Moth (Operophtera brumata L., Lepidoptera: Geometridae) utilizes a single hydrocarbon, 1,Z3,Z6,Z9-nonadecatetraene, as its sex pheromone. We tested the hypothesis that a fatty acid precursor, Z11,Z14,Z17,19-nonadecanoic acid, is biosynthesized from α-linolenic acid, through chain elongation by one 2-carbon unit, and subsequent methyl-terminus desaturation. Our results show that labeled α-linolenic acid is indeed incorporated into the pheromone component in vivo. A fatty-acyl-CoA desaturase gene that we found to be expressed in the abdominal epidermal tissue, the presumed site of biosynthesis for type II pheromones, was characterized and expressed heterologously in a yeast system. The transgenic yeast expressing this... (More)
- The Winter Moth (Operophtera brumata L., Lepidoptera: Geometridae) utilizes a single hydrocarbon, 1,Z3,Z6,Z9-nonadecatetraene, as its sex pheromone. We tested the hypothesis that a fatty acid precursor, Z11,Z14,Z17,19-nonadecanoic acid, is biosynthesized from α-linolenic acid, through chain elongation by one 2-carbon unit, and subsequent methyl-terminus desaturation. Our results show that labeled α-linolenic acid is indeed incorporated into the pheromone component in vivo. A fatty-acyl-CoA desaturase gene that we found to be expressed in the abdominal epidermal tissue, the presumed site of biosynthesis for type II pheromones, was characterized and expressed heterologously in a yeast system. The transgenic yeast expressing this insect derived gene could convert Z11,Z14,Z17-eicosatrienoic acid into Z11,Z14,Z17,19-eicosatetraenoic acid. These results provide evidence that a terminal desaturation step is involved in the winter moth pheromone biosynthesis, prior to the decarboxylation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2008339
- author
- Ding, Bao-Jian
LU
; Liénard, Marjorie
LU
; Wang, Hong-Lei
LU
; Zhao, Cheng-Hua
LU
and Löfstedt, Christer
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Insect Biochemistry and Molecular Biology
- volume
- 41
- pages
- 715 - 722
- publisher
- Elsevier
- external identifiers
-
- wos:000293105000009
- pmid:21651981
- scopus:79959794226
- pmid:21651981
- ISSN
- 1879-0240
- DOI
- 10.1016/j.ibmb.2011.05.003
- project
- The pheromone brewery
- Evolutionary mechanisms of pheromone divergence in Lepidoptera
- language
- English
- LU publication?
- yes
- id
- aac857ba-6d18-4fc5-ac31-f8248a0e7ad1 (old id 2008339)
- date added to LUP
- 2016-04-01 13:44:38
- date last changed
- 2024-05-09 11:38:01
@article{aac857ba-6d18-4fc5-ac31-f8248a0e7ad1,
abstract = {{The Winter Moth (<i>Operophtera brumata</i> L., Lepidoptera: Geometridae) utilizes a single hydrocarbon, 1,Z3,Z6,Z9-nonadecatetraene, as its sex pheromone. We tested the hypothesis that a fatty acid precursor, Z11,Z14,Z17,19-nonadecanoic acid, is biosynthesized from α-linolenic acid, through chain elongation by one 2-carbon unit, and subsequent methyl-terminus desaturation. Our results show that labeled α-linolenic acid is indeed incorporated into the pheromone component in vivo. A fatty-acyl-CoA desaturase gene that we found to be expressed in the abdominal epidermal tissue, the presumed site of biosynthesis for type II pheromones, was characterized and expressed heterologously in a yeast system. The transgenic yeast expressing this insect derived gene could convert Z11,Z14,Z17-eicosatrienoic acid into Z11,Z14,Z17,19-eicosatetraenoic acid. These results provide evidence that a terminal desaturation step is involved in the winter moth pheromone biosynthesis, prior to the decarboxylation.}},
author = {{Ding, Bao-Jian and Liénard, Marjorie and Wang, Hong-Lei and Zhao, Cheng-Hua and Löfstedt, Christer}},
issn = {{1879-0240}},
language = {{eng}},
pages = {{715--722}},
publisher = {{Elsevier}},
series = {{Insect Biochemistry and Molecular Biology}},
title = {{Terminal fatty-acyl-CoA desaturase involved in sex pheromone biosynthesis in the Winter Moth <i>(Operophtera brumata)</i>}},
url = {{http://dx.doi.org/10.1016/j.ibmb.2011.05.003}},
doi = {{10.1016/j.ibmb.2011.05.003}},
volume = {{41}},
year = {{2011}},
}