Genotyping techniques to address diversity in tumors.
Lindgren, David LU ; Höglund, Mattias LU and Vallon-Christersson, Johan LU
(2011)
In Advances in Cancer Research
112.
p.151-182
- Abstract
- Array-based genotyping platforms have during recent years been established as a valuable tool for the characterization of genomic alterations in cancer. The analysis of tumor samples, however, presents challenges for data analysis and interpretation. For example, tumor samples are often admixed with nonaberrant cells that define the tumor microenvironment, such as infiltrating lymphocytes and fibroblasts, or vasculature. Furthermore, tumors often comprise subclones harboring divergent aberrations that are acquired subsequent to the tumor-initiating event. The combined analysis of both genotype and copy number status obtained by array-based genotyping platforms provide opportunities to address these challenges. In this chapter, we present... (More)
- Array-based genotyping platforms have during recent years been established as a valuable tool for the characterization of genomic alterations in cancer. The analysis of tumor samples, however, presents challenges for data analysis and interpretation. For example, tumor samples are often admixed with nonaberrant cells that define the tumor microenvironment, such as infiltrating lymphocytes and fibroblasts, or vasculature. Furthermore, tumors often comprise subclones harboring divergent aberrations that are acquired subsequent to the tumor-initiating event. The combined analysis of both genotype and copy number status obtained by array-based genotyping platforms provide opportunities to address these challenges. In this chapter, we present the basic principles for current array-based genotyping platforms and how they can be used to infer genotype and copy number for acquired genomic alterations. We describe how these techniques can be used to resolve tumor ploidy, normal cell admixture, and subclonality. We also exemplify how genotyping techniques can be applied in tumor studies to elucidate the hierarchy among tumor clones, and thus, provide means to study clonal expansion and tumor evolution. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2168833
- author
- Lindgren, David
LU
; Höglund, Mattias
LU
and Vallon-Christersson, Johan
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Advances in Cancer Research
- volume
- 112
- pages
- 151 - 182
- publisher
- Elsevier
- external identifiers
-
- wos:000295818600006
- pmid:21925304
- scopus:80053000957
- pmid:21925304
- ISSN
- 0065-230X
- DOI
- 10.1016/B978-0-12-387688-1.00006-5
- language
- English
- LU publication?
- yes
- additional info
- Department affilation moved from v1000583 (Molecular Tumour Biology) to v1000562 (Department of Translational Medicine) on 2016-01-18 14:41:46.
- id
- 2203c919-5d88-421c-b7d9-469b0157f8ad (old id 2168833)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21925304?dopt=Abstract
- date added to LUP
- 2016-04-01 15:03:41
- date last changed
- 2022-03-14 17:11:20
@article{2203c919-5d88-421c-b7d9-469b0157f8ad,
abstract = {{Array-based genotyping platforms have during recent years been established as a valuable tool for the characterization of genomic alterations in cancer. The analysis of tumor samples, however, presents challenges for data analysis and interpretation. For example, tumor samples are often admixed with nonaberrant cells that define the tumor microenvironment, such as infiltrating lymphocytes and fibroblasts, or vasculature. Furthermore, tumors often comprise subclones harboring divergent aberrations that are acquired subsequent to the tumor-initiating event. The combined analysis of both genotype and copy number status obtained by array-based genotyping platforms provide opportunities to address these challenges. In this chapter, we present the basic principles for current array-based genotyping platforms and how they can be used to infer genotype and copy number for acquired genomic alterations. We describe how these techniques can be used to resolve tumor ploidy, normal cell admixture, and subclonality. We also exemplify how genotyping techniques can be applied in tumor studies to elucidate the hierarchy among tumor clones, and thus, provide means to study clonal expansion and tumor evolution.}},
author = {{Lindgren, David and Höglund, Mattias and Vallon-Christersson, Johan}},
issn = {{0065-230X}},
language = {{eng}},
pages = {{151--182}},
publisher = {{Elsevier}},
series = {{Advances in Cancer Research}},
title = {{Genotyping techniques to address diversity in tumors.}},
url = {{https://lup.lub.lu.se/search/files/4320874/2831691.pdf}},
doi = {{10.1016/B978-0-12-387688-1.00006-5}},
volume = {{112}},
year = {{2011}},
}