Treatment of Mouse Infants with Amoxicillin, but Not the Human Milk-Derived Antimicrobial HAMLET, Impairs Lung Th17 Responses
(2023) In Antibiotics 12(2).- Abstract
Emerging evidence suggests differential effects of therapeutic antibiotics on infant T cell responses to pathogens. In this study, we explored the impact of the treatment of mouse infants with amoxicillin and the human milk-derived antimicrobial HAMLET (human alpha-lactalbumin made lethal to tumor cells) on T cell responses to
Streptococcus pneumoniae. Lung cells and splenocytes were isolated from the infant mice subjected to intranasal administration of amoxicillin, HAMLET, or a combination of HAMLET and amoxicillin, and cultured with
S. pneumoniae to measure T cell responses. After
in-vitro stimulation with
S.
pneumoniae, lung cells from amoxicillin- or amoxicillin plus HAMLET-treated mice produced lower... (More)Emerging evidence suggests differential effects of therapeutic antibiotics on infant T cell responses to pathogens. In this study, we explored the impact of the treatment of mouse infants with amoxicillin and the human milk-derived antimicrobial HAMLET (human alpha-lactalbumin made lethal to tumor cells) on T cell responses to
(Less)
Streptococcus pneumoniae. Lung cells and splenocytes were isolated from the infant mice subjected to intranasal administration of amoxicillin, HAMLET, or a combination of HAMLET and amoxicillin, and cultured with
S. pneumoniae to measure T cell responses. After
in-vitro stimulation with
S.
pneumoniae, lung cells from amoxicillin- or amoxicillin plus HAMLET-treated mice produced lower levels of Th17 (IL-17A), but not Th1 (IFN-γ), cytokine than mice receiving HAMLET or PBS. IL-17A/IFN-γ cytokine levels produced by the stimulated splenocytes, on the other hand, revealed no significant difference among treatment groups. Further analysis of T cell cytokine profiles by flow cytometry showed that lung CD4+, but not CD8+, T cells from amoxicillin- or HAMLET plus amoxicillin-treated mice expressed decreased levels of IL-17A compared to those from HAMLET-exposed or control mice. Collectively, these results indicate that exposure of infant mice to amoxicillin, but not HAMLET, may suppress lung Th17 responses to
S. pneumoniae.
- author
- Shekhar, Sudhanshu
; Brar, Navdeep Kaur
LU
; Håkansson, Anders P
LU
and Petersen, Fernanda Cristina
- organization
- publishing date
- 2023-02-20
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- amoxicillin, HAMLET, infants, lungs, Th17 immunity
- in
- Antibiotics
- volume
- 12
- issue
- 2
- article number
- 423
- pages
- 11 pages
- publisher
- MDPI AG
- external identifiers
-
- pmid:36830333
- scopus:85148866895
- ISSN
- 2079-6382
- DOI
- 10.3390/antibiotics12020423
- language
- English
- LU publication?
- yes
- id
- 220ee516-5521-4263-b911-51ab14ad9518
- date added to LUP
- 2023-03-03 11:34:06
- date last changed
- 2024-07-12 02:49:39
@article{220ee516-5521-4263-b911-51ab14ad9518, abstract = {{<p>Emerging evidence suggests differential effects of therapeutic antibiotics on infant T cell responses to pathogens. In this study, we explored the impact of the treatment of mouse infants with amoxicillin and the human milk-derived antimicrobial HAMLET (human alpha-lactalbumin made lethal to tumor cells) on T cell responses to <br> Streptococcus pneumoniae. Lung cells and splenocytes were isolated from the infant mice subjected to intranasal administration of amoxicillin, HAMLET, or a combination of HAMLET and amoxicillin, and cultured with <br> S. pneumoniae to measure T cell responses. After <br> in-vitro stimulation with <br> S. <br> pneumoniae, lung cells from amoxicillin- or amoxicillin plus HAMLET-treated mice produced lower levels of Th17 (IL-17A), but not Th1 (IFN-γ), cytokine than mice receiving HAMLET or PBS. IL-17A/IFN-γ cytokine levels produced by the stimulated splenocytes, on the other hand, revealed no significant difference among treatment groups. Further analysis of T cell cytokine profiles by flow cytometry showed that lung CD4+, but not CD8+, T cells from amoxicillin- or HAMLET plus amoxicillin-treated mice expressed decreased levels of IL-17A compared to those from HAMLET-exposed or control mice. Collectively, these results indicate that exposure of infant mice to amoxicillin, but not HAMLET, may suppress lung Th17 responses to<br> S. pneumoniae.<br> </p>}}, author = {{Shekhar, Sudhanshu and Brar, Navdeep Kaur and Håkansson, Anders P and Petersen, Fernanda Cristina}}, issn = {{2079-6382}}, keywords = {{amoxicillin; HAMLET; infants; lungs; Th17 immunity}}, language = {{eng}}, month = {{02}}, number = {{2}}, publisher = {{MDPI AG}}, series = {{Antibiotics}}, title = {{Treatment of Mouse Infants with Amoxicillin, but Not the Human Milk-Derived Antimicrobial HAMLET, Impairs Lung Th17 Responses}}, url = {{http://dx.doi.org/10.3390/antibiotics12020423}}, doi = {{10.3390/antibiotics12020423}}, volume = {{12}}, year = {{2023}}, }