Sodium dodecyl sulfate free gel electrophoresis/electroelution sorting for peptide fractionation
(2019) In Journal of Separation Science 42(24). p.3712-3717- Abstract
Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide gel electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free... (More)
Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide gel electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
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- author
- Ramos, Yassel ; González, Annia ; Sosa-Acosta, Patricia ; Perez-Riverol, Yasset ; García, Yairet ; Castellanos-Serra, Lila ; Gil, Jeovanis LU ; Sánchez, Aniel LU ; González, Luis J. and Besada, Vladimir
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- electrophoresis, peptide fractionation, proteomics
- in
- Journal of Separation Science
- volume
- 42
- issue
- 24
- pages
- 3712 - 3717
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:85074854756
- pmid:31625274
- ISSN
- 1615-9306
- DOI
- 10.1002/jssc.201900495
- language
- English
- LU publication?
- yes
- id
- 2236566e-7425-4ae6-85f5-472ecf32da12
- date added to LUP
- 2019-12-02 14:39:14
- date last changed
- 2024-05-29 04:45:46
@article{2236566e-7425-4ae6-85f5-472ecf32da12,
abstract = {{<p>Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide gel electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.</p>}},
author = {{Ramos, Yassel and González, Annia and Sosa-Acosta, Patricia and Perez-Riverol, Yasset and García, Yairet and Castellanos-Serra, Lila and Gil, Jeovanis and Sánchez, Aniel and González, Luis J. and Besada, Vladimir}},
issn = {{1615-9306}},
keywords = {{electrophoresis; peptide fractionation; proteomics}},
language = {{eng}},
number = {{24}},
pages = {{3712--3717}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Separation Science}},
title = {{Sodium dodecyl sulfate free gel electrophoresis/electroelution sorting for peptide fractionation}},
url = {{http://dx.doi.org/10.1002/jssc.201900495}},
doi = {{10.1002/jssc.201900495}},
volume = {{42}},
year = {{2019}},
}