The calpain inhibitor VI prevents apoptosis of adult motor neurons
(2005) In NeuroReport 16(10). p.1065-1068- Abstract
- Motor neuron cell death was studied in organotypic cultures of adult spinal cord slices from the mouse. Six hours after excision, many motor neuron nuclei displayed apoptotic features including nuclear and chromatin condensation. At this time point, many motor neurons also exhibited immunoreactivity to calpain II. Both the calpain inhibitor VI and ethyleneglycol-bis(beta-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA) prevented the appearance of apoptotic nuclei whereas the pan caspase inhibitor Z-VAD.fmk had no effect. The results suggest that calpain is required for apoptosis of motor neurons and that this type of apoptosis is independent of caspase activation.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/231983
- author
- Momeni, HamidReza LU and Kanje, Martin LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- culture, organ, motor neurons, caspase, calpain inhibitor, apoptosis, calpain, spinal cord
- in
- NeuroReport
- volume
- 16
- issue
- 10
- pages
- 1065 - 1068
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- pmid:15973149
- wos:000230772100007
- scopus:22544456011
- ISSN
- 1473-558X
- language
- English
- LU publication?
- yes
- id
- 4367428f-213b-4e7b-b21c-7726b5a94cd9 (old id 231983)
- date added to LUP
- 2016-04-01 12:23:20
- date last changed
- 2022-01-27 03:05:20
@article{4367428f-213b-4e7b-b21c-7726b5a94cd9, abstract = {{Motor neuron cell death was studied in organotypic cultures of adult spinal cord slices from the mouse. Six hours after excision, many motor neuron nuclei displayed apoptotic features including nuclear and chromatin condensation. At this time point, many motor neurons also exhibited immunoreactivity to calpain II. Both the calpain inhibitor VI and ethyleneglycol-bis(beta-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA) prevented the appearance of apoptotic nuclei whereas the pan caspase inhibitor Z-VAD.fmk had no effect. The results suggest that calpain is required for apoptosis of motor neurons and that this type of apoptosis is independent of caspase activation.}}, author = {{Momeni, HamidReza and Kanje, Martin}}, issn = {{1473-558X}}, keywords = {{culture; organ; motor neurons; caspase; calpain inhibitor; apoptosis; calpain; spinal cord}}, language = {{eng}}, number = {{10}}, pages = {{1065--1068}}, publisher = {{Lippincott Williams & Wilkins}}, series = {{NeuroReport}}, title = {{The calpain inhibitor VI prevents apoptosis of adult motor neurons}}, volume = {{16}}, year = {{2005}}, }