Amyloid-beta Oligomers in Cerebrospinal Fluid are Associated with Cognitive Decline in Patients with Alzheimer's Disease
(2012) In Journal of Alzheimer's Disease 29(1). p.171-176- Abstract
- Oligomers of the amyloid-beta peptide (A beta) are thought to be the most toxic form of A beta and are linked to the development of Alzheimer's disease (AD). Here, we used a flow cytometric approach for the detection and assessment of oligomers in cerebrospinal fluid (CSF) from AD patients and other neurological disorders. 30 CSF samples from patients suffering from AD (n=14), non-demented controls (n=12), and other neurological disorders (dementia with Lewy bodies, n=2; vascular dementia, n=1; primary progressive aphasia, n = 1) were analyzed for the presence of A beta-oligomers by flow cytometry. The CSF levels of total tau (t-tau), phosphorylated tau (p-tau), and amyloid-beta (A beta)(42) were determined using ELISA. CSF A beta-oligomer... (More)
- Oligomers of the amyloid-beta peptide (A beta) are thought to be the most toxic form of A beta and are linked to the development of Alzheimer's disease (AD). Here, we used a flow cytometric approach for the detection and assessment of oligomers in cerebrospinal fluid (CSF) from AD patients and other neurological disorders. 30 CSF samples from patients suffering from AD (n=14), non-demented controls (n=12), and other neurological disorders (dementia with Lewy bodies, n=2; vascular dementia, n=1; primary progressive aphasia, n = 1) were analyzed for the presence of A beta-oligomers by flow cytometry. The CSF levels of total tau (t-tau), phosphorylated tau (p-tau), and amyloid-beta (A beta)(42) were determined using ELISA. CSF A beta-oligomer levels in AD patients were elevated in comparison to the non-AD group (p = 0.073). The ratio A beta-oligomers/A beta(42) was significantly elevated in AD subjects compared to non-AD subjects (p=0.001). Most important, there was a negative correlation between the amount of A beta-oligomers and the Mini-Mental Status Exam score (r=-0.65; p=0.013) in AD patients. The detection of A beta-oligomers using flow cytometry analysis seems to be useful in assessing the stage of AD. This is a novel and important finding as none of the currently used CSF biomarkers are clearly associated with dementia severity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2517551
- author
- Santos, Alexander Navarrete ; Ewers, Michael ; Minthon, Lennart LU ; Simm, Andreas ; Silber, Rolf-Edgar ; Blennow, Kaj ; Prvulovic, David ; Hansson, Oskar LU and Hampel, Harald
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- oligomers, cytometry, flow, cognitive decline, biomarkers, amyloid-beta, Alzheimer's disease
- in
- Journal of Alzheimer's Disease
- volume
- 29
- issue
- 1
- pages
- 171 - 176
- publisher
- IOS Press
- external identifiers
-
- wos:000301139000015
- ISSN
- 1387-2877
- DOI
- 10.3233/JAD-2011-111361
- language
- English
- LU publication?
- yes
- id
- bc13f555-1628-40cc-a4bf-2efbbb1c7cdd (old id 2517551)
- date added to LUP
- 2016-04-01 10:08:11
- date last changed
- 2021-09-27 04:01:32
@article{bc13f555-1628-40cc-a4bf-2efbbb1c7cdd, abstract = {{Oligomers of the amyloid-beta peptide (A beta) are thought to be the most toxic form of A beta and are linked to the development of Alzheimer's disease (AD). Here, we used a flow cytometric approach for the detection and assessment of oligomers in cerebrospinal fluid (CSF) from AD patients and other neurological disorders. 30 CSF samples from patients suffering from AD (n=14), non-demented controls (n=12), and other neurological disorders (dementia with Lewy bodies, n=2; vascular dementia, n=1; primary progressive aphasia, n = 1) were analyzed for the presence of A beta-oligomers by flow cytometry. The CSF levels of total tau (t-tau), phosphorylated tau (p-tau), and amyloid-beta (A beta)(42) were determined using ELISA. CSF A beta-oligomer levels in AD patients were elevated in comparison to the non-AD group (p = 0.073). The ratio A beta-oligomers/A beta(42) was significantly elevated in AD subjects compared to non-AD subjects (p=0.001). Most important, there was a negative correlation between the amount of A beta-oligomers and the Mini-Mental Status Exam score (r=-0.65; p=0.013) in AD patients. The detection of A beta-oligomers using flow cytometry analysis seems to be useful in assessing the stage of AD. This is a novel and important finding as none of the currently used CSF biomarkers are clearly associated with dementia severity.}}, author = {{Santos, Alexander Navarrete and Ewers, Michael and Minthon, Lennart and Simm, Andreas and Silber, Rolf-Edgar and Blennow, Kaj and Prvulovic, David and Hansson, Oskar and Hampel, Harald}}, issn = {{1387-2877}}, keywords = {{oligomers; cytometry; flow; cognitive decline; biomarkers; amyloid-beta; Alzheimer's disease}}, language = {{eng}}, number = {{1}}, pages = {{171--176}}, publisher = {{IOS Press}}, series = {{Journal of Alzheimer's Disease}}, title = {{Amyloid-beta Oligomers in Cerebrospinal Fluid are Associated with Cognitive Decline in Patients with Alzheimer's Disease}}, url = {{http://dx.doi.org/10.3233/JAD-2011-111361}}, doi = {{10.3233/JAD-2011-111361}}, volume = {{29}}, year = {{2012}}, }