Imaging meets cytometry : Analyzing heterogeneous functional microscopic data from living cell populations
(2021) In Journal of Imaging 7(1).- Abstract
Biological tissue consists of populations of cells exhibiting different responses to pharmacological stimuli. To probe the heterogeneity of cell function, we propose a multiplexed approach based on real-time imaging of the secondary messenger levels within each cell of the tissue, followed by extraction of the changes of single-cell fluorescence over time. By utilizing a piecewise baseline correction, we were able to quantify the effects of multiple pharmacological stimuli added and removed sequentially to pancreatic islets of Langerhans, thereby performing a deep functional profiling for each cell within the islet. Cluster analysis based on the functional profile demonstrated dose-dependent changes in statistical inter-relationships... (More)
Biological tissue consists of populations of cells exhibiting different responses to pharmacological stimuli. To probe the heterogeneity of cell function, we propose a multiplexed approach based on real-time imaging of the secondary messenger levels within each cell of the tissue, followed by extraction of the changes of single-cell fluorescence over time. By utilizing a piecewise baseline correction, we were able to quantify the effects of multiple pharmacological stimuli added and removed sequentially to pancreatic islets of Langerhans, thereby performing a deep functional profiling for each cell within the islet. Cluster analysis based on the functional profile demonstrated dose-dependent changes in statistical inter-relationships between islet cell populations. We therefore believe that the functional cytometric approach can be used for routine quantitative profiling of the tissue for drug screening or pathological testing.
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- author
- Draper, Matthew ; Willems, Mara ; Malahe, Reshwan K. ; Hamilton, Alexander LU and Tarasov, Andrei I.
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Antidiabetic drugs, Baseline correction, Cell profiling, Fluorescence, Time-lapse imaging
- in
- Journal of Imaging
- volume
- 7
- issue
- 1
- article number
- 9
- publisher
- MDPI AG
- external identifiers
-
- scopus:85103601776
- pmid:34460580
- ISSN
- 2313-433X
- DOI
- 10.3390/jimaging7010009
- language
- English
- LU publication?
- yes
- id
- 25969c3d-b15d-48b6-bda7-28ac0c780970
- date added to LUP
- 2021-04-13 13:42:47
- date last changed
- 2024-08-10 14:37:56
@article{25969c3d-b15d-48b6-bda7-28ac0c780970,
abstract = {{<p>Biological tissue consists of populations of cells exhibiting different responses to pharmacological stimuli. To probe the heterogeneity of cell function, we propose a multiplexed approach based on real-time imaging of the secondary messenger levels within each cell of the tissue, followed by extraction of the changes of single-cell fluorescence over time. By utilizing a piecewise baseline correction, we were able to quantify the effects of multiple pharmacological stimuli added and removed sequentially to pancreatic islets of Langerhans, thereby performing a deep functional profiling for each cell within the islet. Cluster analysis based on the functional profile demonstrated dose-dependent changes in statistical inter-relationships between islet cell populations. We therefore believe that the functional cytometric approach can be used for routine quantitative profiling of the tissue for drug screening or pathological testing.</p>}},
author = {{Draper, Matthew and Willems, Mara and Malahe, Reshwan K. and Hamilton, Alexander and Tarasov, Andrei I.}},
issn = {{2313-433X}},
keywords = {{Antidiabetic drugs; Baseline correction; Cell profiling; Fluorescence; Time-lapse imaging}},
language = {{eng}},
number = {{1}},
publisher = {{MDPI AG}},
series = {{Journal of Imaging}},
title = {{Imaging meets cytometry : Analyzing heterogeneous functional microscopic data from living cell populations}},
url = {{http://dx.doi.org/10.3390/jimaging7010009}},
doi = {{10.3390/jimaging7010009}},
volume = {{7}},
year = {{2021}},
}