Batch- and continuous propionic acid production from glycerol using free and immobilized cells of Propionibacterium acidipropionici
(2012) In Bioresource Technology 118C. p.553-562- Abstract
- Propionicacid production from glycerol was studied using Propionibacterium acidipropionici DSM 4900 cells immobilized on polyethylenimine-treated Poraver (PEI-Poraver) and Luffa (PEI-Luffa), respectively. Using PEI-Luffa, the average productivity, yield and concentration of propionicacid from 40 g.L-1 glycerol were 0.29 g.L-1.h-1, 0.74 mol.mol-1 and 20.09 g.L-1, respectively, after four consecutive recycle-batches. PEI-Poraver supported attachment of 31 times higher amount of cells than PEI-Luffa and produced 20, 28 and 35 g.L-1propionicacid from 40, 65 and 85 g.L-1 glycerol, respectively (0.61 molPA.molGly-1). The corresponding production rates were 0.86, 0.43 and 0.35 g.L-1.h-1, which are the highest reported from glycerol via batch or... (More)
- Propionicacid production from glycerol was studied using Propionibacterium acidipropionici DSM 4900 cells immobilized on polyethylenimine-treated Poraver (PEI-Poraver) and Luffa (PEI-Luffa), respectively. Using PEI-Luffa, the average productivity, yield and concentration of propionicacid from 40 g.L-1 glycerol were 0.29 g.L-1.h-1, 0.74 mol.mol-1 and 20.09 g.L-1, respectively, after four consecutive recycle-batches. PEI-Poraver supported attachment of 31 times higher amount of cells than PEI-Luffa and produced 20, 28 and 35 g.L-1propionicacid from 40, 65 and 85 g.L-1 glycerol, respectively (0.61 molPA.molGly-1). The corresponding production rates were 0.86, 0.43 and 0.35 g.L-1.h-1, which are the highest reported from glycerol via batch or fed-batch fermentations for equivalent propionicacid concentrations. Using a continuous mode of operation at a dilution rate of 0.1 h-1, cell washout was observed in the bioreactor with free cells; however, propionicacid productivity, yield and concentration were 1.4 g.L-1.h-1, 0.86 molPA.molGly-1, and 14.5 g.L-1, respectively, using immobilized cells in the PEI-Poraver bioreactor. The choice of the immobilization matrix can thus significantly influence the fermentation efficiency and -profile. The bioreactor using cells immobilized on PEI-Poraver allowed the fermentation of higher glycerol concentrations and provided stable and higher fermentation rates than that using free cells or the cells immobilized on PEI-Luffa. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2628888
- author
- Dishisha, Tarek LU ; Alvarez Aliaga, Teresa LU and Hatti-Kaul, Rajni LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Fermentation, Platform chemical, Organic acid, Cell adsorption, Polyethylenimine
- in
- Bioresource Technology
- volume
- 118C
- pages
- 553 - 562
- publisher
- Elsevier
- external identifiers
-
- wos:000307259600074
- scopus:84862653546
- pmid:22728152
- ISSN
- 1873-2976
- DOI
- 10.1016/j.biortech.2012.05.079
- language
- English
- LU publication?
- yes
- id
- 936cae74-ff48-4141-97d1-76246f2a5c48 (old id 2628888)
- date added to LUP
- 2016-04-01 14:37:40
- date last changed
- 2022-04-14 18:50:26
@article{936cae74-ff48-4141-97d1-76246f2a5c48,
abstract = {{Propionicacid production from glycerol was studied using Propionibacterium acidipropionici DSM 4900 cells immobilized on polyethylenimine-treated Poraver (PEI-Poraver) and Luffa (PEI-Luffa), respectively. Using PEI-Luffa, the average productivity, yield and concentration of propionicacid from 40 g.L-1 glycerol were 0.29 g.L-1.h-1, 0.74 mol.mol-1 and 20.09 g.L-1, respectively, after four consecutive recycle-batches. PEI-Poraver supported attachment of 31 times higher amount of cells than PEI-Luffa and produced 20, 28 and 35 g.L-1propionicacid from 40, 65 and 85 g.L-1 glycerol, respectively (0.61 molPA.molGly-1). The corresponding production rates were 0.86, 0.43 and 0.35 g.L-1.h-1, which are the highest reported from glycerol via batch or fed-batch fermentations for equivalent propionicacid concentrations. Using a continuous mode of operation at a dilution rate of 0.1 h-1, cell washout was observed in the bioreactor with free cells; however, propionicacid productivity, yield and concentration were 1.4 g.L-1.h-1, 0.86 molPA.molGly-1, and 14.5 g.L-1, respectively, using immobilized cells in the PEI-Poraver bioreactor. The choice of the immobilization matrix can thus significantly influence the fermentation efficiency and -profile. The bioreactor using cells immobilized on PEI-Poraver allowed the fermentation of higher glycerol concentrations and provided stable and higher fermentation rates than that using free cells or the cells immobilized on PEI-Luffa.}},
author = {{Dishisha, Tarek and Alvarez Aliaga, Teresa and Hatti-Kaul, Rajni}},
issn = {{1873-2976}},
keywords = {{Fermentation; Platform chemical; Organic acid; Cell adsorption; Polyethylenimine}},
language = {{eng}},
pages = {{553--562}},
publisher = {{Elsevier}},
series = {{Bioresource Technology}},
title = {{Batch- and continuous propionic acid production from glycerol using free and immobilized cells of Propionibacterium acidipropionici}},
url = {{https://lup.lub.lu.se/search/files/4073436/4937831.pdf}},
doi = {{10.1016/j.biortech.2012.05.079}},
volume = {{118C}},
year = {{2012}},
}