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Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements

Kanno, T ; Ma, Xiaosong LU ; Barg, Sebastian LU ; Eliasson, Lena LU orcid ; Galvanovskis, Juris LU ; Göpel, Sven LU ; Larsson, Max LU ; Renström, Erik LU and Rorsman, Patrik LU (2004) In Methods 33(4). p.302-311
Abstract
This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic P-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other. (C) 2004 Elsevier Inc. All rights reserved.
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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods
volume
33
issue
4
pages
302 - 311
publisher
Elsevier
external identifiers
  • wos:000222459900007
  • pmid:15183179
  • scopus:2442717004
ISSN
1095-9130
DOI
10.1016/j.ymeth.2004.01.003
language
English
LU publication?
yes
id
5656c048-62fb-4393-8fb6-d8665febb900 (old id 273466)
date added to LUP
2016-04-01 12:27:27
date last changed
2022-01-27 05:21:02
@article{5656c048-62fb-4393-8fb6-d8665febb900,
  abstract     = {{This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic P-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other. (C) 2004 Elsevier Inc. All rights reserved.}},
  author       = {{Kanno, T and Ma, Xiaosong and Barg, Sebastian and Eliasson, Lena and Galvanovskis, Juris and Göpel, Sven and Larsson, Max and Renström, Erik and Rorsman, Patrik}},
  issn         = {{1095-9130}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{302--311}},
  publisher    = {{Elsevier}},
  series       = {{Methods}},
  title        = {{Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements}},
  url          = {{http://dx.doi.org/10.1016/j.ymeth.2004.01.003}},
  doi          = {{10.1016/j.ymeth.2004.01.003}},
  volume       = {{33}},
  year         = {{2004}},
}