Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements
(2004) In Methods 33(4). p.302-311- Abstract
- This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic P-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other. (C) 2004 Elsevier Inc. All rights reserved.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/273466
- author
- Kanno, T ; Ma, Xiaosong LU ; Barg, Sebastian LU ; Eliasson, Lena LU ; Galvanovskis, Juris LU ; Göpel, Sven LU ; Larsson, Max LU ; Renström, Erik LU and Rorsman, Patrik LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Methods
- volume
- 33
- issue
- 4
- pages
- 302 - 311
- publisher
- Elsevier
- external identifiers
-
- wos:000222459900007
- pmid:15183179
- scopus:2442717004
- ISSN
- 1095-9130
- DOI
- 10.1016/j.ymeth.2004.01.003
- language
- English
- LU publication?
- yes
- id
- 5656c048-62fb-4393-8fb6-d8665febb900 (old id 273466)
- date added to LUP
- 2016-04-01 12:27:27
- date last changed
- 2022-01-27 05:21:02
@article{5656c048-62fb-4393-8fb6-d8665febb900, abstract = {{This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic P-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other. (C) 2004 Elsevier Inc. All rights reserved.}}, author = {{Kanno, T and Ma, Xiaosong and Barg, Sebastian and Eliasson, Lena and Galvanovskis, Juris and Göpel, Sven and Larsson, Max and Renström, Erik and Rorsman, Patrik}}, issn = {{1095-9130}}, language = {{eng}}, number = {{4}}, pages = {{302--311}}, publisher = {{Elsevier}}, series = {{Methods}}, title = {{Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements}}, url = {{http://dx.doi.org/10.1016/j.ymeth.2004.01.003}}, doi = {{10.1016/j.ymeth.2004.01.003}}, volume = {{33}}, year = {{2004}}, }