The major glucagon-like peptide-1 metabolite, GLP-1-(9-36)-amide, does not affect glucose or insulin levels in mice
(2004) In European Journal of Pharmacology 494(2-3). p.283-288- Abstract
- Glucagon-like peptide-1 (GLP-1), a future treatment for type 2 diabetes, is efficiently degraded by the enzyme dipeptidyl peptidase IV (DPP IV), yielding the major metabolite GLP-1-(9-36)-amide. In this study, we examined the potential glucose lowering effect of GLP-1(9-36)-amide in mice and found that GLP-1-(9-36)-amide (3 and 10 nmol/kg) did not affect insulin secretion or glucose elimination when administered intravenously together with glucose (1 g/kg). This was observed both in normal mice and in transgenic mice having a complete disruption of the signalling from the GLP-1 receptor. Furthermore, after blocking insulin secretion, using diazoxide (25 mg/kg), no effect on insulin-independent glucose disposal of GLP-1-(9-36)-amide was... (More)
- Glucagon-like peptide-1 (GLP-1), a future treatment for type 2 diabetes, is efficiently degraded by the enzyme dipeptidyl peptidase IV (DPP IV), yielding the major metabolite GLP-1-(9-36)-amide. In this study, we examined the potential glucose lowering effect of GLP-1(9-36)-amide in mice and found that GLP-1-(9-36)-amide (3 and 10 nmol/kg) did not affect insulin secretion or glucose elimination when administered intravenously together with glucose (1 g/kg). This was observed both in normal mice and in transgenic mice having a complete disruption of the signalling from the GLP-1 receptor. Furthermore, after blocking insulin secretion, using diazoxide (25 mg/kg), no effect on insulin-independent glucose disposal of GLP-1-(9-36)-amide was observed. Therefore, GLP-1-(9-36)-amide does not affect glucose disposal in mice either in the presence or absence of intact GLP-1-receptors or in the presence or absence of stimulated insulin levels. This suggests that the GLP-1 metabolite is not involved in the regulation of glucose homeostasis. (C) 2004 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/273580
- author
- Rolin, B ; Deacon, CF ; Carr, RD and Ahrén, Bo LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- transgenic animal, glucagon like peptide 1, dipeptidyl peptidase IV, model, diabetes
- in
- European Journal of Pharmacology
- volume
- 494
- issue
- 2-3
- pages
- 283 - 288
- publisher
- Elsevier
- external identifiers
-
- wos:000222398600025
- pmid:15212985
- scopus:2942755813
- pmid:15212985
- ISSN
- 1879-0712
- DOI
- 10.1016/j.ejphar.2004.05.013
- language
- English
- LU publication?
- yes
- id
- b645fa64-83dd-4844-a267-1c38e7cba147 (old id 273580)
- date added to LUP
- 2016-04-01 11:36:52
- date last changed
- 2024-02-06 02:22:53
@article{b645fa64-83dd-4844-a267-1c38e7cba147, abstract = {{Glucagon-like peptide-1 (GLP-1), a future treatment for type 2 diabetes, is efficiently degraded by the enzyme dipeptidyl peptidase IV (DPP IV), yielding the major metabolite GLP-1-(9-36)-amide. In this study, we examined the potential glucose lowering effect of GLP-1(9-36)-amide in mice and found that GLP-1-(9-36)-amide (3 and 10 nmol/kg) did not affect insulin secretion or glucose elimination when administered intravenously together with glucose (1 g/kg). This was observed both in normal mice and in transgenic mice having a complete disruption of the signalling from the GLP-1 receptor. Furthermore, after blocking insulin secretion, using diazoxide (25 mg/kg), no effect on insulin-independent glucose disposal of GLP-1-(9-36)-amide was observed. Therefore, GLP-1-(9-36)-amide does not affect glucose disposal in mice either in the presence or absence of intact GLP-1-receptors or in the presence or absence of stimulated insulin levels. This suggests that the GLP-1 metabolite is not involved in the regulation of glucose homeostasis. (C) 2004 Elsevier B.V. All rights reserved.}}, author = {{Rolin, B and Deacon, CF and Carr, RD and Ahrén, Bo}}, issn = {{1879-0712}}, keywords = {{transgenic animal; glucagon like peptide 1; dipeptidyl peptidase IV; model; diabetes}}, language = {{eng}}, number = {{2-3}}, pages = {{283--288}}, publisher = {{Elsevier}}, series = {{European Journal of Pharmacology}}, title = {{The major glucagon-like peptide-1 metabolite, GLP-1-(9-36)-amide, does not affect glucose or insulin levels in mice}}, url = {{http://dx.doi.org/10.1016/j.ejphar.2004.05.013}}, doi = {{10.1016/j.ejphar.2004.05.013}}, volume = {{494}}, year = {{2004}}, }