The TCF7L2-dependent high-voltage activated calcium channel subunit α2δ-1 controls calcium signaling in rodent pancreatic beta-cells
Yingying, Ye LU ; Barghouth, Mohammad LU ; Luan, Cheng LU ; Kazim, Abdulla LU ; Zhou, Yuedan LU ; Eliasson, Lena LU
; Zhang, Enming
LU
; Hansson, Ola
LU
; Thevenin, Thomas
LU
and Renström, Erik
LU
(2020)
In Molecular and Cellular Endocrinology
502.
p.1-11
- Abstract
- The transcription factor TCF7L2 remains the most important diabetes gene identified to date and genetic risk carriers exhibit lower insulin secretion. We show that Tcf7l2 regulates the auxiliary subunit of voltage-gated Ca2+ channels, Cacna2d1 gene/α2δ-1 protein levels. Furthermore, suppression of α2δ-1 decreased voltage-gated Ca2+ currents and high glucose/depolarization-evoked Ca2+ signaling which mimicked the effect of silencing of Tcf7l2. This appears to be the result of impaired voltage-gated Ca2+ channel trafficking to the plasma membrane, as Cav1.2 channels accumulated in the recycling endosomes after α2δ-1 suppression, in clonal as well as primary rodent beta-cells. This impaired the capacity for glucose-induced insulin secretion... (More)
- The transcription factor TCF7L2 remains the most important diabetes gene identified to date and genetic risk carriers exhibit lower insulin secretion. We show that Tcf7l2 regulates the auxiliary subunit of voltage-gated Ca2+ channels, Cacna2d1 gene/α2δ-1 protein levels. Furthermore, suppression of α2δ-1 decreased voltage-gated Ca2+ currents and high glucose/depolarization-evoked Ca2+ signaling which mimicked the effect of silencing of Tcf7l2. This appears to be the result of impaired voltage-gated Ca2+ channel trafficking to the plasma membrane, as Cav1.2 channels accumulated in the recycling endosomes after α2δ-1 suppression, in clonal as well as primary rodent beta-cells. This impaired the capacity for glucose-induced insulin secretion in Cacna2d1-silenced cells. Overexpression of α2δ-1 increased high-glucose/K+-stimulated insulin secretion. Furthermore, overexpression of α2δ-1 in Tcf7l2-silenced cells rescued the Tcf7l2-dependent impairment of Ca2+ signaling, but not the reduced insulin secretion. Taken together, these data clarify the connection between Tcf7l2, α2δ-1 in Ca2+-dependent insulin secretion. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/293bc097-7b63-4c4b-9775-77aa6fceb664
- author
- Yingying, Ye
LU
; Barghouth, Mohammad
LU
; Luan, Cheng
LU
; Kazim, Abdulla
LU
; Zhou, Yuedan
LU
; Eliasson, Lena
LU
; Zhang, Enming
LU
; Hansson, Ola
LU
; Thevenin, Thomas
LU
and Renström, Erik
LU
- organization
- publishing date
- 2020-02-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- TCF7L2, α2δ-1, Type 2 Diabetes
- in
- Molecular and Cellular Endocrinology
- volume
- 502
- article number
- 110673
- pages
- 1 - 11
- publisher
- Elsevier
- external identifiers
-
- scopus:85076248475
- pmid:31805307
- ISSN
- 0303-7207
- DOI
- 10.1016/j.mce.2019.110673
- language
- English
- LU publication?
- yes
- id
- 293bc097-7b63-4c4b-9775-77aa6fceb664
- date added to LUP
- 2020-01-08 13:35:28
- date last changed
- 2024-03-20 03:01:41
@article{293bc097-7b63-4c4b-9775-77aa6fceb664,
abstract = {{The transcription factor TCF7L2 remains the most important diabetes gene identified to date and genetic risk carriers exhibit lower insulin secretion. We show that Tcf7l2 regulates the auxiliary subunit of voltage-gated Ca2+ channels, Cacna2d1 gene/α2δ-1 protein levels. Furthermore, suppression of α2δ-1 decreased voltage-gated Ca2+ currents and high glucose/depolarization-evoked Ca2+ signaling which mimicked the effect of silencing of Tcf7l2. This appears to be the result of impaired voltage-gated Ca2+ channel trafficking to the plasma membrane, as Cav1.2 channels accumulated in the recycling endosomes after α2δ-1 suppression, in clonal as well as primary rodent beta-cells. This impaired the capacity for glucose-induced insulin secretion in Cacna2d1-silenced cells. Overexpression of α2δ-1 increased high-glucose/K+-stimulated insulin secretion. Furthermore, overexpression of α2δ-1 in Tcf7l2-silenced cells rescued the Tcf7l2-dependent impairment of Ca2+ signaling, but not the reduced insulin secretion. Taken together, these data clarify the connection between Tcf7l2, α2δ-1 in Ca2+-dependent insulin secretion.}},
author = {{Yingying, Ye and Barghouth, Mohammad and Luan, Cheng and Kazim, Abdulla and Zhou, Yuedan and Eliasson, Lena and Zhang, Enming and Hansson, Ola and Thevenin, Thomas and Renström, Erik}},
issn = {{0303-7207}},
keywords = {{TCF7L2; α2δ-1; Type 2 Diabetes}},
language = {{eng}},
month = {{02}},
pages = {{1--11}},
publisher = {{Elsevier}},
series = {{Molecular and Cellular Endocrinology}},
title = {{The TCF7L2-dependent high-voltage activated calcium channel subunit α2δ-1 controls calcium signaling in rodent pancreatic beta-cells}},
url = {{http://dx.doi.org/10.1016/j.mce.2019.110673}},
doi = {{10.1016/j.mce.2019.110673}},
volume = {{502}},
year = {{2020}},
}