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Identification of stage-specific surface markers in early B cell development provides novel tools for identification of progenitor populations

Jensen, Christina T. LU ; Lang, Stefan LU orcid ; Somasundaram, Rajesh ; Soneji, Shamit LU and Sigvardsson, Mikael LU (2016) In Journal of Immunology 197(5). p.1937-1944
Abstract

Whereas the characterization of B lymphoid progenitors has been facilitated by the identification of lineage-and stage-specific surface markers, the continued identification of differentially expressed proteins increases our capacity to explore normal and malignant B cell development. To identify novel surface markers with stage-specific expression patterns, we explored the reactivity of CD19+ B cell progenitor cells to Abs targeted to 176 surface proteins. Markers with stage-specific expression were identified using a transgenic reporter gene system subdividing the B cell progenitors into four surface IgM2 stages. This approach affirmed the utility of known stage-specific markers, as well as identifying additional proteins that... (More)

Whereas the characterization of B lymphoid progenitors has been facilitated by the identification of lineage-and stage-specific surface markers, the continued identification of differentially expressed proteins increases our capacity to explore normal and malignant B cell development. To identify novel surface markers with stage-specific expression patterns, we explored the reactivity of CD19+ B cell progenitor cells to Abs targeted to 176 surface proteins. Markers with stage-specific expression were identified using a transgenic reporter gene system subdividing the B cell progenitors into four surface IgM2 stages. This approach affirmed the utility of known stage-specific markers, as well as identifying additional proteins that selectively marked defined stages of B cell development. Among the stage-specific markers were the cell adhesion proteins CD49E, CD11A, and CD54 that are highly expressed selectively on the most immature progenitors. This work identifies a set of novel stage-specific surface markers that can be used as a complement to the classical staining protocols to explore B lymphocyte development.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Immunology
volume
197
issue
5
pages
8 pages
publisher
American Association of Immunologists
external identifiers
  • pmid:27456481
  • wos:000385002300041
  • scopus:84983786111
ISSN
0022-1767
DOI
10.4049/jimmunol.1600297
language
English
LU publication?
yes
id
2c9c03e2-0f90-4f6a-937e-ca49eb9bb75e
date added to LUP
2016-12-06 11:34:59
date last changed
2024-07-12 21:59:14
@article{2c9c03e2-0f90-4f6a-937e-ca49eb9bb75e,
  abstract     = {{<p>Whereas the characterization of B lymphoid progenitors has been facilitated by the identification of lineage-and stage-specific surface markers, the continued identification of differentially expressed proteins increases our capacity to explore normal and malignant B cell development. To identify novel surface markers with stage-specific expression patterns, we explored the reactivity of CD19+ B cell progenitor cells to Abs targeted to 176 surface proteins. Markers with stage-specific expression were identified using a transgenic reporter gene system subdividing the B cell progenitors into four surface IgM2 stages. This approach affirmed the utility of known stage-specific markers, as well as identifying additional proteins that selectively marked defined stages of B cell development. Among the stage-specific markers were the cell adhesion proteins CD49E, CD11A, and CD54 that are highly expressed selectively on the most immature progenitors. This work identifies a set of novel stage-specific surface markers that can be used as a complement to the classical staining protocols to explore B lymphocyte development.</p>}},
  author       = {{Jensen, Christina T. and Lang, Stefan and Somasundaram, Rajesh and Soneji, Shamit and Sigvardsson, Mikael}},
  issn         = {{0022-1767}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{5}},
  pages        = {{1937--1944}},
  publisher    = {{American Association of Immunologists}},
  series       = {{Journal of Immunology}},
  title        = {{Identification of stage-specific surface markers in early B cell development provides novel tools for identification of progenitor populations}},
  url          = {{http://dx.doi.org/10.4049/jimmunol.1600297}},
  doi          = {{10.4049/jimmunol.1600297}},
  volume       = {{197}},
  year         = {{2016}},
}