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Tetratricopeptide-containing SMALL KERNEL 11 is essential for the assembly of cytochrome c oxidase in maize mitochondria

Ren, Zhenjing ; Fan, Kaijian ; Zhen, Sihan ; Zhang, Jie ; Liu, Yan ; Fu, Junjie ; Qi, Chunlai ; Wei, Qianhan ; Du, Yao and Tatar, Wurinile , et al. (2023) In Plant Physiology 192(1). p.170-187
Abstract

Assembly of the functional complexes of the mitochondrial respiratory chain requires sophisticated and efficient regulatory mechanisms. In plants, the subunit composition and assembly factors involved in the biogenesis of cytochrome c oxidase (complex IV) are substantially less defined than in mammals and yeast. In this study, we cloned maize (Zea mays) Small kernel 11 (Smk11) via map-based cloning. Smk11 encodes a mitochondria-localized tetratricopeptide repeat protein. Disruption of Smk11 severely affected the assembly and activity of mitochondrial complex IV, leading to delayed plant growth and seed development. Protein interactions studies revealed that SMK11 might interact with four putative complex IV assembly factors, Inner... (More)

Assembly of the functional complexes of the mitochondrial respiratory chain requires sophisticated and efficient regulatory mechanisms. In plants, the subunit composition and assembly factors involved in the biogenesis of cytochrome c oxidase (complex IV) are substantially less defined than in mammals and yeast. In this study, we cloned maize (Zea mays) Small kernel 11 (Smk11) via map-based cloning. Smk11 encodes a mitochondria-localized tetratricopeptide repeat protein. Disruption of Smk11 severely affected the assembly and activity of mitochondrial complex IV, leading to delayed plant growth and seed development. Protein interactions studies revealed that SMK11 might interact with four putative complex IV assembly factors, Inner membrane peptidase 1A (ZmIMP1A), MYB domain protein 3R3 (ZmMYB3R-3), cytochrome c oxidase 23 (ZmCOX23), and mitochondrial ferredoxin 1 (ZmMFDX1), among which ZmMFDX1 might interact with subunits ZmCOX6a and ZmCOX-X1; ZmMYB3R-3 might also interact with ZmCOX6a. The mutation of SMK11 perturbed the normal assembly of these subunits, leading to the inactivation of complex IV. The results of this study revealed that SMK11 serves as an accessory assembly factor required for the normal assembly of subunits into complex IV, which will accelerate the elucidation of the assembly of complex IV in plant mitochondria.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Plant Physiology
volume
192
issue
1
pages
18 pages
publisher
American Society of Plant Biologists
external identifiers
  • pmid:36722259
  • scopus:85158843924
ISSN
0032-0889
DOI
10.1093/plphys/kiad062
language
English
LU publication?
yes
id
318b63c8-c8b0-4ddc-93e0-c0972226d7d2
date added to LUP
2023-08-11 09:57:13
date last changed
2025-01-26 04:34:09
@article{318b63c8-c8b0-4ddc-93e0-c0972226d7d2,
  abstract     = {{<p>Assembly of the functional complexes of the mitochondrial respiratory chain requires sophisticated and efficient regulatory mechanisms. In plants, the subunit composition and assembly factors involved in the biogenesis of cytochrome c oxidase (complex IV) are substantially less defined than in mammals and yeast. In this study, we cloned maize (Zea mays) Small kernel 11 (Smk11) via map-based cloning. Smk11 encodes a mitochondria-localized tetratricopeptide repeat protein. Disruption of Smk11 severely affected the assembly and activity of mitochondrial complex IV, leading to delayed plant growth and seed development. Protein interactions studies revealed that SMK11 might interact with four putative complex IV assembly factors, Inner membrane peptidase 1A (ZmIMP1A), MYB domain protein 3R3 (ZmMYB3R-3), cytochrome c oxidase 23 (ZmCOX23), and mitochondrial ferredoxin 1 (ZmMFDX1), among which ZmMFDX1 might interact with subunits ZmCOX6a and ZmCOX-X1; ZmMYB3R-3 might also interact with ZmCOX6a. The mutation of SMK11 perturbed the normal assembly of these subunits, leading to the inactivation of complex IV. The results of this study revealed that SMK11 serves as an accessory assembly factor required for the normal assembly of subunits into complex IV, which will accelerate the elucidation of the assembly of complex IV in plant mitochondria.</p>}},
  author       = {{Ren, Zhenjing and Fan, Kaijian and Zhen, Sihan and Zhang, Jie and Liu, Yan and Fu, Junjie and Qi, Chunlai and Wei, Qianhan and Du, Yao and Tatar, Wurinile and Zhang, Xiaofeng and Wang, Guoying and Rasmusson, Allan G. and Wang, Jianhua and Liu, Yunjun}},
  issn         = {{0032-0889}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{170--187}},
  publisher    = {{American Society of Plant Biologists}},
  series       = {{Plant Physiology}},
  title        = {{Tetratricopeptide-containing SMALL KERNEL 11 is essential for the assembly of cytochrome c oxidase in maize mitochondria}},
  url          = {{http://dx.doi.org/10.1093/plphys/kiad062}},
  doi          = {{10.1093/plphys/kiad062}},
  volume       = {{192}},
  year         = {{2023}},
}