Lipase action on a monoolein/sodium oleate aqueous cubic liquid crystalline phase - a NMR and X-ray diffraction study
(2002) In Colloids and Surfaces B: Biointerfaces 26(1-2). p.159-171- Abstract
- The effect of adding Thermomyces (formerly Humicola) lanuginosa lipase (TLL) to a monoolein (MO)/sodium oleate (NaO) aqueous cubic liquid crystalline phase has been studied. H-1-NMR, C-13-NMR, H-1-PGSE (Pulsed-magnetic field Gradient Spin-Echo) self-diffusion measurements, and Small Angle X-ray Diffraction were used to follow the degradation of the cubic phase. The reaction sequence in terms of phase transitions follows the order bicontinuous cubic --> reverse hexagonal --> micellar cubic --> micellar phase and corresponds to the previously determined phase diagrams. These changes correlate with changes in the lipid composition observed by C-13-NMR and confirmed by HPLC analysis. The initial decrease of the diffusion coefficients... (More)
- The effect of adding Thermomyces (formerly Humicola) lanuginosa lipase (TLL) to a monoolein (MO)/sodium oleate (NaO) aqueous cubic liquid crystalline phase has been studied. H-1-NMR, C-13-NMR, H-1-PGSE (Pulsed-magnetic field Gradient Spin-Echo) self-diffusion measurements, and Small Angle X-ray Diffraction were used to follow the degradation of the cubic phase. The reaction sequence in terms of phase transitions follows the order bicontinuous cubic --> reverse hexagonal --> micellar cubic --> micellar phase and corresponds to the previously determined phase diagrams. These changes correlate with changes in the lipid composition observed by C-13-NMR and confirmed by HPLC analysis. The initial decrease of the diffusion coefficients of water and lipid can be related to the transformation of the bicontinuous cubic phase to a reverse hexagonal structure. (C) 2002 Elsevier Science B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/322664
- author
- Caboi, F ; Borné, Johanna LU ; Nylander, Tommy LU ; Khan, Ali LU ; Svendsen, A and Patkar, S
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- self-assembly structure, lipolysis, cubic phase, lipase, monoolein
- in
- Colloids and Surfaces B: Biointerfaces
- volume
- 26
- issue
- 1-2
- pages
- 159 - 171
- publisher
- Elsevier
- external identifiers
-
- wos:000179487200016
- scopus:0036074332
- ISSN
- 1873-4367
- DOI
- 10.1016/S0927-7765(02)00035-8
- language
- English
- LU publication?
- yes
- id
- aa29a0c6-0751-4b95-badd-9ecb8325b69b (old id 322664)
- date added to LUP
- 2016-04-01 16:29:27
- date last changed
- 2022-01-28 20:06:48
@article{aa29a0c6-0751-4b95-badd-9ecb8325b69b, abstract = {{The effect of adding Thermomyces (formerly Humicola) lanuginosa lipase (TLL) to a monoolein (MO)/sodium oleate (NaO) aqueous cubic liquid crystalline phase has been studied. H-1-NMR, C-13-NMR, H-1-PGSE (Pulsed-magnetic field Gradient Spin-Echo) self-diffusion measurements, and Small Angle X-ray Diffraction were used to follow the degradation of the cubic phase. The reaction sequence in terms of phase transitions follows the order bicontinuous cubic --> reverse hexagonal --> micellar cubic --> micellar phase and corresponds to the previously determined phase diagrams. These changes correlate with changes in the lipid composition observed by C-13-NMR and confirmed by HPLC analysis. The initial decrease of the diffusion coefficients of water and lipid can be related to the transformation of the bicontinuous cubic phase to a reverse hexagonal structure. (C) 2002 Elsevier Science B.V. All rights reserved.}}, author = {{Caboi, F and Borné, Johanna and Nylander, Tommy and Khan, Ali and Svendsen, A and Patkar, S}}, issn = {{1873-4367}}, keywords = {{self-assembly structure; lipolysis; cubic phase; lipase; monoolein}}, language = {{eng}}, number = {{1-2}}, pages = {{159--171}}, publisher = {{Elsevier}}, series = {{Colloids and Surfaces B: Biointerfaces}}, title = {{Lipase action on a monoolein/sodium oleate aqueous cubic liquid crystalline phase - a NMR and X-ray diffraction study}}, url = {{http://dx.doi.org/10.1016/S0927-7765(02)00035-8}}, doi = {{10.1016/S0927-7765(02)00035-8}}, volume = {{26}}, year = {{2002}}, }