Chromosomal integration and expression of green fluorescent protein in Zymomonas mobilis
(2002) In Biotechnology Letters 24(15). p.1285-1290- Abstract
- An integrative vector was constructed to allow expression of heterologous proteins into the adhB locus of Zymomonas mobilis. As a reporter gene, the ORF of a bright variant of green fluorescent protein from Aequorea victoria (GFPuv) was fused to the adhB strong promoter from Z. mobilis by using a two-step PCR strategy. Z. mobilis recombinant strains that were stably marked by precise gene replacement at adhB locus with a single chromosomal copy of gfpuv. Protein expression was confirmed by fluorescence microscopy and measured by fluorescence spectroscopy, showing high expression levels (12 to 30 times higher than those obtained in E. coli) without affecting the host growth.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/333874
- author
- Delgado, Osvaldo LU ; Martinez, MA ; Abate, CM and Sineriz, F
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- PCR, Zymomonas mobilis, gfpuv, alcohol dehydrogenase, expression vector
- in
- Biotechnology Letters
- volume
- 24
- issue
- 15
- pages
- 1285 - 1290
- publisher
- Springer
- external identifiers
-
- wos:000176804800012
- scopus:0036332032
- ISSN
- 1573-6776
- DOI
- 10.1023/A:1016266110391
- language
- English
- LU publication?
- yes
- id
- 5ee18abd-45af-48c9-a252-07c978bc4fef (old id 333874)
- date added to LUP
- 2016-04-01 16:22:57
- date last changed
- 2022-01-28 19:17:47
@article{5ee18abd-45af-48c9-a252-07c978bc4fef, abstract = {{An integrative vector was constructed to allow expression of heterologous proteins into the adhB locus of Zymomonas mobilis. As a reporter gene, the ORF of a bright variant of green fluorescent protein from Aequorea victoria (GFPuv) was fused to the adhB strong promoter from Z. mobilis by using a two-step PCR strategy. Z. mobilis recombinant strains that were stably marked by precise gene replacement at adhB locus with a single chromosomal copy of gfpuv. Protein expression was confirmed by fluorescence microscopy and measured by fluorescence spectroscopy, showing high expression levels (12 to 30 times higher than those obtained in E. coli) without affecting the host growth.}}, author = {{Delgado, Osvaldo and Martinez, MA and Abate, CM and Sineriz, F}}, issn = {{1573-6776}}, keywords = {{PCR; Zymomonas mobilis; gfpuv; alcohol dehydrogenase; expression vector}}, language = {{eng}}, number = {{15}}, pages = {{1285--1290}}, publisher = {{Springer}}, series = {{Biotechnology Letters}}, title = {{Chromosomal integration and expression of green fluorescent protein in Zymomonas mobilis}}, url = {{http://dx.doi.org/10.1023/A:1016266110391}}, doi = {{10.1023/A:1016266110391}}, volume = {{24}}, year = {{2002}}, }