At low water activity alpha-chymotrypsin is more active in an ionic liquid than in non-ionic organic solvents
(2002) In Biotechnology Letters 24(11). p.867-872- Abstract
- The kinetics of the alpha-chymotrypsin catalysed transesterification of N-acetyl-l-phenylalanine ethyl ester with 1-butanol and the competing hydrolysis were evaluated at fixed water activity in two ionic liquids and two non-ionic organic solvents. In most respects the four solvents behaved similarly. However, at a water activity of 0.33, higher catalytic activity was observed in the ionic liquid, 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide, than in the other solvents, and at a(w)=0.11 catalysis was only observed in this solvent.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/337279
- author
- Eckstein, M
; Sesing, M
; Kragl, U
and Adlercreutz, Patrick
LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- water activity, alpha-chymotrypsin, ionic liquids
- in
- Biotechnology Letters
- volume
- 24
- issue
- 11
- pages
- 867 - 872
- publisher
- Springer
- external identifiers
-
- wos:000175752100003
- scopus:0036273739
- ISSN
- 1573-6776
- DOI
- 10.1023/A:1015564608261
- language
- English
- LU publication?
- yes
- id
- 014ecc11-1a14-4228-94f5-3ed36bb1d0c2 (old id 337279)
- date added to LUP
- 2016-04-01 16:43:19
- date last changed
- 2022-04-15 06:38:17
@article{014ecc11-1a14-4228-94f5-3ed36bb1d0c2, abstract = {{The kinetics of the alpha-chymotrypsin catalysed transesterification of N-acetyl-l-phenylalanine ethyl ester with 1-butanol and the competing hydrolysis were evaluated at fixed water activity in two ionic liquids and two non-ionic organic solvents. In most respects the four solvents behaved similarly. However, at a water activity of 0.33, higher catalytic activity was observed in the ionic liquid, 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide, than in the other solvents, and at a(w)=0.11 catalysis was only observed in this solvent.}}, author = {{Eckstein, M and Sesing, M and Kragl, U and Adlercreutz, Patrick}}, issn = {{1573-6776}}, keywords = {{water activity; alpha-chymotrypsin; ionic liquids}}, language = {{eng}}, number = {{11}}, pages = {{867--872}}, publisher = {{Springer}}, series = {{Biotechnology Letters}}, title = {{At low water activity alpha-chymotrypsin is more active in an ionic liquid than in non-ionic organic solvents}}, url = {{http://dx.doi.org/10.1023/A:1015564608261}}, doi = {{10.1023/A:1015564608261}}, volume = {{24}}, year = {{2002}}, }