Effects of calcium deficiency and calcium supplementation on gastrectomy-induced osteopenia in the young male rat
(2002) In Scandinavian Journal of Gastroenterology 37(3). p.299-306- Abstract
- Background: Surgical removal of the stomach (gastrectomy, Gx) induces osteopenia. In this study we compared the osteopenic effect of Gx with that induced by calcium (Ca) deficiency. Methods: Young male rats were subjected to Gx and/or low Ca diet (-Ca). A group of Gx rats received standard diet + oral Ca supplementation (+ Ca). The rats were killed at various times after the operation/start of treatment (longest time 12 weeks). After 8 weeks on low Ca diet, the blood Ca 2 concentration was lowered slightly in both Sham-operated and Gx rats. The calvariac were subjected to transillumination analysis and quantitative histomorphometry. Also the tibiae were subjected to histomorphometry. Results: Transillumination of the calvariae revealed... (More)
- Background: Surgical removal of the stomach (gastrectomy, Gx) induces osteopenia. In this study we compared the osteopenic effect of Gx with that induced by calcium (Ca) deficiency. Methods: Young male rats were subjected to Gx and/or low Ca diet (-Ca). A group of Gx rats received standard diet + oral Ca supplementation (+ Ca). The rats were killed at various times after the operation/start of treatment (longest time 12 weeks). After 8 weeks on low Ca diet, the blood Ca 2 concentration was lowered slightly in both Sham-operated and Gx rats. The calvariac were subjected to transillumination analysis and quantitative histomorphometry. Also the tibiae were subjected to histomorphometry. Results: Transillumination of the calvariae revealed extensive bone loss in the rats that had been subjected to Gx and/or low Ca diet, Gx + Ca induced the same bone loss as Gx alone. These observations were later confirmed in quantitative terms by histomorphometry (Sham-Ca 56%, Gx 35%, Gx + Ca 32%, Gx - Ca 58% less bone area than in Sham). The osteopenia induced by Gx+ low Ca diet seemed more rapid in onset than that induced by Gx or low Ca diet alone. Tibiae from Gx rats and rats given a low Ca diet displayed a reduced trabecular bone volume (Sham-Ca 27%, remaining, Gx 36%, Gx + Ca 44%, Gx - Ca 1711) and reduced trabecular number (Sham-Ca 44% remaining, Gx 41%, Gx + Ca 56%, Gx - Ca 33%). The trabecular thickness was reduced in the Gx rats and Gx - Ca rats (Gx 78% remaining, Gx-Ca 63%) but not in Sham-operated rats receiving a low Ca-dict (95% remaining). Conclusion: Although the pattern of osteopenia was qualitatively quite similar in Gx rats and Ca-deficient rats, in quantitative terms the low Ca diet was more detrimental to bone than Gx. Ca deficiency induced a similar degree of osteopenia in both Sham and Gx rats. Ca supplementation failed to prevent the Gx-induced osteopenia. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/342409
- author
- Lehto-Axtelius, D ; Surve, Vikas LU ; Johnell, Olof LU and Håkanson, Rolf LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- gastrectomy, calvaria, calcium supplementation, bone, calcium deficiency, osteopenia, rats, tibia
- in
- Scandinavian Journal of Gastroenterology
- volume
- 37
- issue
- 3
- pages
- 299 - 306
- publisher
- Taylor & Francis
- external identifiers
-
- pmid:11916192
- wos:000174212400008
- scopus:0036188568
- ISSN
- 1502-7708
- DOI
- 10.1080/003655202317284200
- language
- English
- LU publication?
- yes
- id
- 7a78f1f9-87ab-40ff-aa23-09a80356ccfe (old id 342409)
- date added to LUP
- 2016-04-01 16:05:36
- date last changed
- 2024-03-14 21:21:54
@article{7a78f1f9-87ab-40ff-aa23-09a80356ccfe,
abstract = {{Background: Surgical removal of the stomach (gastrectomy, Gx) induces osteopenia. In this study we compared the osteopenic effect of Gx with that induced by calcium (Ca) deficiency. Methods: Young male rats were subjected to Gx and/or low Ca diet (-Ca). A group of Gx rats received standard diet + oral Ca supplementation (+ Ca). The rats were killed at various times after the operation/start of treatment (longest time 12 weeks). After 8 weeks on low Ca diet, the blood Ca 2 concentration was lowered slightly in both Sham-operated and Gx rats. The calvariac were subjected to transillumination analysis and quantitative histomorphometry. Also the tibiae were subjected to histomorphometry. Results: Transillumination of the calvariae revealed extensive bone loss in the rats that had been subjected to Gx and/or low Ca diet, Gx + Ca induced the same bone loss as Gx alone. These observations were later confirmed in quantitative terms by histomorphometry (Sham-Ca 56%, Gx 35%, Gx + Ca 32%, Gx - Ca 58% less bone area than in Sham). The osteopenia induced by Gx+ low Ca diet seemed more rapid in onset than that induced by Gx or low Ca diet alone. Tibiae from Gx rats and rats given a low Ca diet displayed a reduced trabecular bone volume (Sham-Ca 27%, remaining, Gx 36%, Gx + Ca 44%, Gx - Ca 1711) and reduced trabecular number (Sham-Ca 44% remaining, Gx 41%, Gx + Ca 56%, Gx - Ca 33%). The trabecular thickness was reduced in the Gx rats and Gx - Ca rats (Gx 78% remaining, Gx-Ca 63%) but not in Sham-operated rats receiving a low Ca-dict (95% remaining). Conclusion: Although the pattern of osteopenia was qualitatively quite similar in Gx rats and Ca-deficient rats, in quantitative terms the low Ca diet was more detrimental to bone than Gx. Ca deficiency induced a similar degree of osteopenia in both Sham and Gx rats. Ca supplementation failed to prevent the Gx-induced osteopenia.}},
author = {{Lehto-Axtelius, D and Surve, Vikas and Johnell, Olof and Håkanson, Rolf}},
issn = {{1502-7708}},
keywords = {{gastrectomy; calvaria; calcium supplementation; bone; calcium deficiency; osteopenia; rats; tibia}},
language = {{eng}},
number = {{3}},
pages = {{299--306}},
publisher = {{Taylor & Francis}},
series = {{Scandinavian Journal of Gastroenterology}},
title = {{Effects of calcium deficiency and calcium supplementation on gastrectomy-induced osteopenia in the young male rat}},
url = {{http://dx.doi.org/10.1080/003655202317284200}},
doi = {{10.1080/003655202317284200}},
volume = {{37}},
year = {{2002}},
}