Leucine increases muscle mitochondrial respiration and attenuates glucose intolerance in diet-induced obesity in Swiss mice
(2019) In Journal of Functional Foods 62.- Abstract
Leucine is an essential amino acid that has been investigated by its participation in the regulation of whole-body metabolism and mitochondrial function. Here, we evaluated acute leucine effects on mitochondrial respiration of skeletal muscle from male Swiss mice in vitro. Additionally, we further investigated the effects of 4-wk leucine ingestion (2.5% on drinking water) on skeletal muscle mitochondrial respiration and morphology of diet-induced obesity (DIO) mice. In vitro, acute leucine increased mitochondrial respiration, and these effects were abolished in the presence of rapamycin. In DIO mice, ingestion of leucine for 4-wk improved glucose tolerance and insulin responsivity. Leucine supplementation also prevented the reduction in... (More)
Leucine is an essential amino acid that has been investigated by its participation in the regulation of whole-body metabolism and mitochondrial function. Here, we evaluated acute leucine effects on mitochondrial respiration of skeletal muscle from male Swiss mice in vitro. Additionally, we further investigated the effects of 4-wk leucine ingestion (2.5% on drinking water) on skeletal muscle mitochondrial respiration and morphology of diet-induced obesity (DIO) mice. In vitro, acute leucine increased mitochondrial respiration, and these effects were abolished in the presence of rapamycin. In DIO mice, ingestion of leucine for 4-wk improved glucose tolerance and insulin responsivity. Leucine supplementation also prevented the reduction in mitochondrial respiration, size, and complexity in the soleus skeletal muscle. We conclude that the positive effects of leucine on whole-body metabolism in DIO mice are associated with improvements in skeletal muscle mitochondrial function and morphology. Furthermore, leucine acute effects on mitochondrial respiration are mTORC1 dependent.
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- author
- Brunetta, Henver Simionato ; de Paula, Gabriela Cristina LU ; Fritzen, Matheus ; Cecchini, Manuela Sozo ; Santos, Gustavo Jorge dos ; Nazari, Evelise Maria ; Rafacho, Alex ; de Bem, Andreza Fabro and Nunes, Everson Araújo
- publishing date
- 2019-11
- type
- Contribution to journal
- publication status
- published
- keywords
- High-fat diet, Insulin resistance, Leucine, Mitochondria, Obesity
- in
- Journal of Functional Foods
- volume
- 62
- article number
- 103544
- publisher
- Elsevier
- external identifiers
-
- scopus:85071418457
- ISSN
- 1756-4646
- DOI
- 10.1016/j.jff.2019.103544
- language
- English
- LU publication?
- no
- additional info
- Funding Information: Brazilian National Council for Scientific and Technological Development (CNPq), grant numbers 304274/2014-3 and 302692/2016-9 . A.F.B., A.R., E. M. N., and E.A.N. are CNPq Research Productivity Fellows. H. S. B. and G. C. P. PhDs scholarships were funded by the Coordination for the Improvement of Higher Education Personnel (CAPES) – Brazil. Funding Information: We thank Dr. Rodrigo Bayne Leal, from the Department of Biochemistry (Federal University of Santa Catarina) for the donation of rapamycin. We thank Logan Townsend for the careful revision of the manuscript. Also, we are thankful for having access to the infrastructure and receiving support during data acquisition in the Central Laboratory of Electron Microscopy (UFSC) and in the Multi-user Laboratory of Biology Studies (UFSC). The authors declare that they have no conflict of interest. Brazilian National Council for Scientific and Technological Development (CNPq), grant numbers 304274/2014-3 and 302692/2016-9. A.F.B. A.R. E. M. N. and E.A.N. are CNPq Research Productivity Fellows. H. S. B. and G. C. P. PhDs scholarships were funded by the Coordination for the Improvement of Higher Education Personnel (CAPES) ? Brazil. Publisher Copyright: © 2019 Elsevier Ltd Copyright: Copyright 2019 Elsevier B.V., All rights reserved.
- id
- 34c16e64-c7ae-4622-ad2d-2ab3dddf4b20
- date added to LUP
- 2021-09-21 19:52:14
- date last changed
- 2022-04-19 08:16:53
@article{34c16e64-c7ae-4622-ad2d-2ab3dddf4b20,
abstract = {{<p>Leucine is an essential amino acid that has been investigated by its participation in the regulation of whole-body metabolism and mitochondrial function. Here, we evaluated acute leucine effects on mitochondrial respiration of skeletal muscle from male Swiss mice in vitro. Additionally, we further investigated the effects of 4-wk leucine ingestion (2.5% on drinking water) on skeletal muscle mitochondrial respiration and morphology of diet-induced obesity (DIO) mice. In vitro, acute leucine increased mitochondrial respiration, and these effects were abolished in the presence of rapamycin. In DIO mice, ingestion of leucine for 4-wk improved glucose tolerance and insulin responsivity. Leucine supplementation also prevented the reduction in mitochondrial respiration, size, and complexity in the soleus skeletal muscle. We conclude that the positive effects of leucine on whole-body metabolism in DIO mice are associated with improvements in skeletal muscle mitochondrial function and morphology. Furthermore, leucine acute effects on mitochondrial respiration are mTORC1 dependent.</p>}},
author = {{Brunetta, Henver Simionato and de Paula, Gabriela Cristina and Fritzen, Matheus and Cecchini, Manuela Sozo and Santos, Gustavo Jorge dos and Nazari, Evelise Maria and Rafacho, Alex and de Bem, Andreza Fabro and Nunes, Everson Araújo}},
issn = {{1756-4646}},
keywords = {{High-fat diet; Insulin resistance; Leucine; Mitochondria; Obesity}},
language = {{eng}},
publisher = {{Elsevier}},
series = {{Journal of Functional Foods}},
title = {{Leucine increases muscle mitochondrial respiration and attenuates glucose intolerance in diet-induced obesity in Swiss mice}},
url = {{http://dx.doi.org/10.1016/j.jff.2019.103544}},
doi = {{10.1016/j.jff.2019.103544}},
volume = {{62}},
year = {{2019}},
}