Estrogen attenuates vascular expression of inflammation associated genes and adhesion of monocytes to endothelial cells
Gao, H. ; Liang, Min LU ; Bergdahl, Andreas LU ; Hamren, A ; Lindholm, Marie LU ; Dahlman-Wright, K. and Nilsson, Bengt-Olof LU
(2006)
In Inflammation Research
55(8).
p.349-353
- Abstract
- Objective: Investigate effects of estrogen at gene expression and functional levels in vascular wall cells treated with bacterial lipopolysaccharide (LPS). Materials and methods: Aortic segments from ovariectomized mice were treated with LPS for 24 h in the absence or presence of 17 beta-estradiol (E-2). Gene activity was determined by Affymetrix microarray analysis and real-time RTPCR. Adhesion of [H-3]-thymidine labelled human THP-1 monocytes to mouse bEnd.3 endothelial cells was determined by measuring radioactivity of DNA from co-culture homogenates. Results: Analysis of global gene expression profiles revealed that 10 nM E-2 attenuates LPS-induced (10 ng/ml) expression of genes coding for well-known acute-phase proteins, such as... (More)
- Objective: Investigate effects of estrogen at gene expression and functional levels in vascular wall cells treated with bacterial lipopolysaccharide (LPS). Materials and methods: Aortic segments from ovariectomized mice were treated with LPS for 24 h in the absence or presence of 17 beta-estradiol (E-2). Gene activity was determined by Affymetrix microarray analysis and real-time RTPCR. Adhesion of [H-3]-thymidine labelled human THP-1 monocytes to mouse bEnd.3 endothelial cells was determined by measuring radioactivity of DNA from co-culture homogenates. Results: Analysis of global gene expression profiles revealed that 10 nM E-2 attenuates LPS-induced (10 ng/ml) expression of genes coding for well-known acute-phase proteins, such as alpha-trypsin inhibitor heavy chain 4, serum amyloid A3 and lipocalin 2. The E-2-induced down-regulation of these three genes observed by microarray was confirmed by realtime RT-PCR. Treatment with 500ng/ml LPS increased adhesion of monocytes to endothelial cells more than two fold. Importantly, LPS-induced monocyte adhesion was fully prevented by 50nM E-2. Conclusion: Estrogen reduces expression of acute-phase protein genes and inhibits LPS-induced moncocyte adhesion to endothelial cells, suggesting that estrogen might have a vasculoprotective effect via this mechanism. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/397425
- author
- Gao, H.
; Liang, Min
LU
; Bergdahl, Andreas
LU
; Hamren, A
; Lindholm, Marie
LU
; Dahlman-Wright, K.
and Nilsson, Bengt-Olof
LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- gene expression-LPS, inflammation, arteries, estrogen
- in
- Inflammation Research
- volume
- 55
- issue
- 8
- pages
- 349 - 353
- publisher
- Birkhäuser Verlag
- external identifiers
-
- wos:000239928400005
- scopus:33748851388
- ISSN
- 1420-908X
- DOI
- 10.1007/s00011-006-5194-z
- language
- English
- LU publication?
- yes
- id
- 9e4f0aba-f7db-4f13-8dcd-b3ac682ac7a9 (old id 397425)
- date added to LUP
- 2016-04-01 12:12:16
- date last changed
- 2022-03-05 20:22:14
@article{9e4f0aba-f7db-4f13-8dcd-b3ac682ac7a9,
abstract = {{Objective: Investigate effects of estrogen at gene expression and functional levels in vascular wall cells treated with bacterial lipopolysaccharide (LPS). Materials and methods: Aortic segments from ovariectomized mice were treated with LPS for 24 h in the absence or presence of 17 beta-estradiol (E-2). Gene activity was determined by Affymetrix microarray analysis and real-time RTPCR. Adhesion of [H-3]-thymidine labelled human THP-1 monocytes to mouse bEnd.3 endothelial cells was determined by measuring radioactivity of DNA from co-culture homogenates. Results: Analysis of global gene expression profiles revealed that 10 nM E-2 attenuates LPS-induced (10 ng/ml) expression of genes coding for well-known acute-phase proteins, such as alpha-trypsin inhibitor heavy chain 4, serum amyloid A3 and lipocalin 2. The E-2-induced down-regulation of these three genes observed by microarray was confirmed by realtime RT-PCR. Treatment with 500ng/ml LPS increased adhesion of monocytes to endothelial cells more than two fold. Importantly, LPS-induced monocyte adhesion was fully prevented by 50nM E-2. Conclusion: Estrogen reduces expression of acute-phase protein genes and inhibits LPS-induced moncocyte adhesion to endothelial cells, suggesting that estrogen might have a vasculoprotective effect via this mechanism.}},
author = {{Gao, H. and Liang, Min and Bergdahl, Andreas and Hamren, A and Lindholm, Marie and Dahlman-Wright, K. and Nilsson, Bengt-Olof}},
issn = {{1420-908X}},
keywords = {{gene expression-LPS; inflammation; arteries; estrogen}},
language = {{eng}},
number = {{8}},
pages = {{349--353}},
publisher = {{Birkhäuser Verlag}},
series = {{Inflammation Research}},
title = {{Estrogen attenuates vascular expression of inflammation associated genes and adhesion of monocytes to endothelial cells}},
url = {{http://dx.doi.org/10.1007/s00011-006-5194-z}},
doi = {{10.1007/s00011-006-5194-z}},
volume = {{55}},
year = {{2006}},
}