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Chitosan flocculation associated with biofilms of C. saccharolyticus and C. owensensis enhances biomass retention in a CSTR

Vongkampang, Thitiwut LU ; Seshagiri Rao, Nikhil LU ; Grey, Carl LU orcid and van Niel, Ed W.J. LU (2021) In International Journal of Hydrogen Energy 46(41). p.21338-21347
Abstract
Cell immobilization and co-culture techniques have gained attention due to its potential to obtain high volumetric hydrogen productivities (QH2). Chitosan retained biomass in the fermentation of co-cultures of Caldicellulosiruptor saccharolyticus and C. owensensis efficiently, up to a maximum dilution rate (D) of 0.9 h−1. Without chitosan, wash out of the co-culture occurred earlier, accompanied with approximately 50% drop in QH2 (D > 0.4 h−1). However, butyl rubber did not show as much potential as carrier material; it did neither improve QH2 nor biomass retention in continuous culture. The population dynamics revealed that C. owensensis was the... (More)
Cell immobilization and co-culture techniques have gained attention due to its potential to obtain high volumetric hydrogen productivities (QH2). Chitosan retained biomass in the fermentation of co-cultures of Caldicellulosiruptor saccharolyticus and C. owensensis efficiently, up to a maximum dilution rate (D) of 0.9 h−1. Without chitosan, wash out of the co-culture occurred earlier, accompanied with approximately 50% drop in QH2 (D > 0.4 h−1). However, butyl rubber did not show as much potential as carrier material; it did neither improve QH2 nor biomass retention in continuous culture. The population dynamics revealed that C. owensensis was the dominant species (95%) in the presence of chitosan, whereas C. saccharolyticus was the predominant (99%) during cultivation without chitosan. In contrast, the co-culture with rubber as carrier maintained the relative population ratios around 1:1. This study highlighted chitosan as an effective potential carrier for immobilization, thereby paving the way for cost – effective hydrogen production. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Caldicellulosiruptor saccharolyticus, Caldicellulosiruptor owensensis, chitosan, biofilm, immobilization, co-culture
in
International Journal of Hydrogen Energy
volume
46
issue
41
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:85106604463
ISSN
1879-3487
DOI
10.1016/j.ijhydene.2021.03.230
language
English
LU publication?
yes
id
39fb2cce-cf73-4e88-a934-274ead4eb973
date added to LUP
2021-06-03 08:09:32
date last changed
2024-10-07 00:03:04
@article{39fb2cce-cf73-4e88-a934-274ead4eb973,
  abstract     = {{Cell immobilization and co-culture techniques have gained attention due to its potential to obtain high volumetric hydrogen productivities (Q<sub>H2</sub>). Chitosan retained biomass in the fermentation of co-cultures of <i>Caldicellulosiruptor saccharolyticus</i> and <i>C. owensensis </i>efficiently, up to a maximum dilution rate (<i>D</i>) of 0.9 h<sup>−1</sup>. Without chitosan, wash out of the co-culture occurred earlier, accompanied with approximately 50% drop in Q<sub>H2</sub> (<i>D</i> &gt; 0.4 h<sup>−1</sup>). However, butyl rubber did not show as much potential as carrier material; it did neither improve Q<sub>H2</sub> nor biomass retention in continuous culture. The population dynamics revealed that <i>C. owensensis</i> was the dominant species (95%) in the presence of chitosan, whereas <i>C. saccharolyticus</i> was the predominant (99%) during cultivation without chitosan. In contrast, the co-culture with rubber as carrier maintained the relative population ratios around 1:1. This study highlighted chitosan as an effective potential carrier for immobilization, thereby paving the way for cost – effective hydrogen production.}},
  author       = {{Vongkampang, Thitiwut and Seshagiri Rao, Nikhil and Grey, Carl and van Niel, Ed W.J.}},
  issn         = {{1879-3487}},
  keywords     = {{Caldicellulosiruptor saccharolyticus; Caldicellulosiruptor owensensis; chitosan; biofilm; immobilization; co-culture}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{41}},
  pages        = {{21338--21347}},
  publisher    = {{Elsevier}},
  series       = {{International Journal of Hydrogen Energy}},
  title        = {{Chitosan flocculation associated with biofilms of <i>C. saccharolyticus</i> and <i>C. owensensis</i> enhances biomass retention in a CSTR}},
  url          = {{http://dx.doi.org/10.1016/j.ijhydene.2021.03.230}},
  doi          = {{10.1016/j.ijhydene.2021.03.230}},
  volume       = {{46}},
  year         = {{2021}},
}