Utility of G protein-coupled receptor 35 expression for predicting outcome in colon cancer
(2019) In Tumor Biology 41(6). p.1-11- Abstract
The utility of mRNA and protein determinations of G protein-coupled receptor 35, that is, GPR35a (GPR35 V1) and GPR35b (GPR35 V2/3), as indicators of outcome for colon cancer patients after curative surgery was investigated. Expression levels of V1 and V2/3 GPR35, carcinoembryonic antigen and CXCL17 mRNAs were assessed in primary tumours and regional lymph nodes of 121 colon cancer patients (stage I–IV), colon cancer cell lines and control colon epithelial cells using real-time quantitative reverse transcriptase-polymerase chain reaction. Expression of G protein-coupled receptor 35 was investigated by two-colour immunohistochemistry and immunomorphometry. GPR35 V2/3 mRNA, but not V1 mRNA, was expressed in colon cancer cell lines,... (More)
The utility of mRNA and protein determinations of G protein-coupled receptor 35, that is, GPR35a (GPR35 V1) and GPR35b (GPR35 V2/3), as indicators of outcome for colon cancer patients after curative surgery was investigated. Expression levels of V1 and V2/3 GPR35, carcinoembryonic antigen and CXCL17 mRNAs were assessed in primary tumours and regional lymph nodes of 121 colon cancer patients (stage I–IV), colon cancer cell lines and control colon epithelial cells using real-time quantitative reverse transcriptase-polymerase chain reaction. Expression of G protein-coupled receptor 35 was investigated by two-colour immunohistochemistry and immunomorphometry. GPR35 V2/3 mRNA, but not V1 mRNA, was expressed in colon cancer cell lines, primary colon tumours and control colon epithelial cells. Haematoxylin and eosin positive (H&E(+)), but not H&E(–), lymph nodes expressed high levels of GPR35 V2/3 mRNA (P<0.0001). GPR35b and carcinoembryonic antigen proteins were simultaneously expressed in many colon cancer tumour cells. Kaplan–Meier and hazard ratio analysis revealed that patients with lymph nodes expressing high levels of GPR35 V2/3 mRNA and, in particular, in the group of patients with lymph nodes also expressing carcinoembryonic antigen mRNA, had a short disease-free survival time, 67 months versus 122 months at 12-year follow-up (difference: 55 months, P = 0.001; hazard ratio: 3.6, P = 0.002). In conclusion, high level expression of G protein-coupled receptor 35 V2/3 mRNA in regional lymph nodes of colon cancer patients is a sign of poor prognosis.
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- author
- Ali, Haytham ; AbdelMageed, Manar ; Olsson, Lina LU ; Israelsson, Anne ; Lindmark, Gudrun LU ; Hammarström, Marie Louise ; Hammarström, Sten and Sitohy, Basel
- organization
- publishing date
- 2019-06-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- CEA, colon cancer, CXCL17 mRNA, GPR35 V1 mRNA, GPR35 V2/3 mRNA, GPR35b, immunohistochemistry, qRT-PCR
- in
- Tumor Biology
- volume
- 41
- issue
- 6
- pages
- 1 - 11
- publisher
- Springer
- external identifiers
-
- scopus:85068211995
- pmid:31250711
- ISSN
- 1010-4283
- DOI
- 10.1177/1010428319858885
- language
- English
- LU publication?
- yes
- id
- 3b8afa0a-0f04-49b1-810c-a44ddf0fea40
- date added to LUP
- 2019-07-09 16:52:25
- date last changed
- 2024-04-16 15:53:21
@article{3b8afa0a-0f04-49b1-810c-a44ddf0fea40, abstract = {{<p>The utility of mRNA and protein determinations of G protein-coupled receptor 35, that is, GPR35a (GPR35 V1) and GPR35b (GPR35 V2/3), as indicators of outcome for colon cancer patients after curative surgery was investigated. Expression levels of V1 and V2/3 GPR35, carcinoembryonic antigen and CXCL17 mRNAs were assessed in primary tumours and regional lymph nodes of 121 colon cancer patients (stage I–IV), colon cancer cell lines and control colon epithelial cells using real-time quantitative reverse transcriptase-polymerase chain reaction. Expression of G protein-coupled receptor 35 was investigated by two-colour immunohistochemistry and immunomorphometry. GPR35 V2/3 mRNA, but not V1 mRNA, was expressed in colon cancer cell lines, primary colon tumours and control colon epithelial cells. Haematoxylin and eosin positive (H&E(+)), but not H&E(–), lymph nodes expressed high levels of GPR35 V2/3 mRNA (P<0.0001). GPR35b and carcinoembryonic antigen proteins were simultaneously expressed in many colon cancer tumour cells. Kaplan–Meier and hazard ratio analysis revealed that patients with lymph nodes expressing high levels of GPR35 V2/3 mRNA and, in particular, in the group of patients with lymph nodes also expressing carcinoembryonic antigen mRNA, had a short disease-free survival time, 67 months versus 122 months at 12-year follow-up (difference: 55 months, P = 0.001; hazard ratio: 3.6, P = 0.002). In conclusion, high level expression of G protein-coupled receptor 35 V2/3 mRNA in regional lymph nodes of colon cancer patients is a sign of poor prognosis.</p>}}, author = {{Ali, Haytham and AbdelMageed, Manar and Olsson, Lina and Israelsson, Anne and Lindmark, Gudrun and Hammarström, Marie Louise and Hammarström, Sten and Sitohy, Basel}}, issn = {{1010-4283}}, keywords = {{CEA; colon cancer; CXCL17 mRNA; GPR35 V1 mRNA; GPR35 V2/3 mRNA; GPR35b; immunohistochemistry; qRT-PCR}}, language = {{eng}}, month = {{06}}, number = {{6}}, pages = {{1--11}}, publisher = {{Springer}}, series = {{Tumor Biology}}, title = {{Utility of G protein-coupled receptor 35 expression for predicting outcome in colon cancer}}, url = {{http://dx.doi.org/10.1177/1010428319858885}}, doi = {{10.1177/1010428319858885}}, volume = {{41}}, year = {{2019}}, }