Laccase-based biosensors for monitoring lignin
(2006) In Enzyme and Microbial Technology 39(4). p.835-840- Abstract
- Four different ways to detect Kraft and soluble pine lignin using either soluble or immobilised Trametes hirsuta laccase have been investigated. In the first, the concentration of lignin was measured using laccase in homogeneous medium with a Clark electrode. In the second, laccase immobilised onto aminated porous glass beads was used in a reactor submerged into the lignin containing solution and the reaction was followed with a Clark electrode. The third and fourth techniques involved lignin detection using laccase modified spectrographic graphite electrodes, either used in flow injection mode or in batch mode, where lignin solutions were injected into an electrochemical cell. It was shown that the use of laccase modified graphite... (More)
- Four different ways to detect Kraft and soluble pine lignin using either soluble or immobilised Trametes hirsuta laccase have been investigated. In the first, the concentration of lignin was measured using laccase in homogeneous medium with a Clark electrode. In the second, laccase immobilised onto aminated porous glass beads was used in a reactor submerged into the lignin containing solution and the reaction was followed with a Clark electrode. The third and fourth techniques involved lignin detection using laccase modified spectrographic graphite electrodes, either used in flow injection mode or in batch mode, where lignin solutions were injected into an electrochemical cell. It was shown that the use of laccase modified graphite electrodes in conjunction with flow injection for amperometric lignin detection is an attractive perspective and our data form the basis for designing laccase-based biosensors to be applied for measuring lignin and its model compounds in waste water, e.g. from the pulp and paper industry. (c) 2006 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/402227
- author
- Shleev, Sergey LU ; Persson, Per LU ; Shumakovich, Galina ; Mazhugo, Yulia ; Yaropolov, Alexander ; Ruzgas, Tautgirdas LU and Gorton, Lo LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- amperometry, biosensor, lignin, laccase
- in
- Enzyme and Microbial Technology
- volume
- 39
- issue
- 4
- pages
- 835 - 840
- publisher
- Elsevier
- external identifiers
-
- wos:000238932500048
- scopus:33745225002
- ISSN
- 0141-0229
- DOI
- 10.1016/j.enzmictec.2006.01.009
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 82d0b483-06b4-4563-a9de-1e829f4bd89b (old id 402227)
- date added to LUP
- 2016-04-01 11:51:43
- date last changed
- 2022-04-13 02:30:04
@article{82d0b483-06b4-4563-a9de-1e829f4bd89b,
abstract = {{Four different ways to detect Kraft and soluble pine lignin using either soluble or immobilised Trametes hirsuta laccase have been investigated. In the first, the concentration of lignin was measured using laccase in homogeneous medium with a Clark electrode. In the second, laccase immobilised onto aminated porous glass beads was used in a reactor submerged into the lignin containing solution and the reaction was followed with a Clark electrode. The third and fourth techniques involved lignin detection using laccase modified spectrographic graphite electrodes, either used in flow injection mode or in batch mode, where lignin solutions were injected into an electrochemical cell. It was shown that the use of laccase modified graphite electrodes in conjunction with flow injection for amperometric lignin detection is an attractive perspective and our data form the basis for designing laccase-based biosensors to be applied for measuring lignin and its model compounds in waste water, e.g. from the pulp and paper industry. (c) 2006 Elsevier Inc. All rights reserved.}},
author = {{Shleev, Sergey and Persson, Per and Shumakovich, Galina and Mazhugo, Yulia and Yaropolov, Alexander and Ruzgas, Tautgirdas and Gorton, Lo}},
issn = {{0141-0229}},
keywords = {{amperometry; biosensor; lignin; laccase}},
language = {{eng}},
number = {{4}},
pages = {{835--840}},
publisher = {{Elsevier}},
series = {{Enzyme and Microbial Technology}},
title = {{Laccase-based biosensors for monitoring lignin}},
url = {{http://dx.doi.org/10.1016/j.enzmictec.2006.01.009}},
doi = {{10.1016/j.enzmictec.2006.01.009}},
volume = {{39}},
year = {{2006}},
}