Surface proteins of the Group G Streptococcus in different phases of growth: patterns of production and implications for the host-bacteria relationship.
Wollein Waldetoft, Kristofer LU ; Karlsson, Christofer LU ; Gram, Magnus LU
; Malmström, Johan
LU
; Mörgelin, Matthias
LU
; Frick, Inga-Maria
LU
and Björck, Lars
LU
(2014)
In Microbiology
160(Nov 12).
p.279-286
- Abstract
- Group G streptococcus (GGS) is a human bacterial pathogen expressing surface proteins FOG and protein G (PG) which interact with several host defense systems, including the complement and contact systems. Selected reaction monitoring mass spectrometry, electron microscopy, and protein binding assays were used to follow the amounts of FOG and PG intracellularly and on the bacterial surface during different phases of growth. Large and increasing amounts of PG were present on the surface in the stationary growth phase, and this was due to de novo production. In contrast, the amount of FOG did not change substantially during this phase. Apart from PG, a number of housekeeping proteins also increased in abundance in the stationary phase. These... (More)
- Group G streptococcus (GGS) is a human bacterial pathogen expressing surface proteins FOG and protein G (PG) which interact with several host defense systems, including the complement and contact systems. Selected reaction monitoring mass spectrometry, electron microscopy, and protein binding assays were used to follow the amounts of FOG and PG intracellularly and on the bacterial surface during different phases of growth. Large and increasing amounts of PG were present on the surface in the stationary growth phase, and this was due to de novo production. In contrast, the amount of FOG did not change substantially during this phase. Apart from PG, a number of housekeeping proteins also increased in abundance in the stationary phase. These results show that GGS protein production is active during the stationary phase and that the bacteria actively remodel their surface and enter a less pro-inflammatory state in this phase. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4179479
- author
- Wollein Waldetoft, Kristofer
LU
; Karlsson, Christofer
LU
; Gram, Magnus
LU
; Malmström, Johan
LU
; Mörgelin, Matthias
LU
; Frick, Inga-Maria
LU
and Björck, Lars
LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Microbiology
- volume
- 160
- issue
- Nov 12
- pages
- 279 - 286
- publisher
- MAIK Nauka/Interperiodica
- external identifiers
-
- pmid:24222616
- wos:000338603100004
- scopus:84893505203
- ISSN
- 1465-2080
- DOI
- 10.1099/mic.0.071332-0
- language
- English
- LU publication?
- yes
- id
- 8fbd6ec7-ed76-4e49-ace8-5b63af839d1e (old id 4179479)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24222616?dopt=Abstract
- date added to LUP
- 2016-04-01 10:40:10
- date last changed
- 2022-01-26 01:19:00
@article{8fbd6ec7-ed76-4e49-ace8-5b63af839d1e,
abstract = {{Group G streptococcus (GGS) is a human bacterial pathogen expressing surface proteins FOG and protein G (PG) which interact with several host defense systems, including the complement and contact systems. Selected reaction monitoring mass spectrometry, electron microscopy, and protein binding assays were used to follow the amounts of FOG and PG intracellularly and on the bacterial surface during different phases of growth. Large and increasing amounts of PG were present on the surface in the stationary growth phase, and this was due to de novo production. In contrast, the amount of FOG did not change substantially during this phase. Apart from PG, a number of housekeeping proteins also increased in abundance in the stationary phase. These results show that GGS protein production is active during the stationary phase and that the bacteria actively remodel their surface and enter a less pro-inflammatory state in this phase.}},
author = {{Wollein Waldetoft, Kristofer and Karlsson, Christofer and Gram, Magnus and Malmström, Johan and Mörgelin, Matthias and Frick, Inga-Maria and Björck, Lars}},
issn = {{1465-2080}},
language = {{eng}},
number = {{Nov 12}},
pages = {{279--286}},
publisher = {{MAIK Nauka/Interperiodica}},
series = {{Microbiology}},
title = {{Surface proteins of the Group G Streptococcus in different phases of growth: patterns of production and implications for the host-bacteria relationship.}},
url = {{http://dx.doi.org/10.1099/mic.0.071332-0}},
doi = {{10.1099/mic.0.071332-0}},
volume = {{160}},
year = {{2014}},
}