Mobility of Core Water in Bacillus subtilis Spores by (2)H NMR.
(2013) In Biophysical Journal 105(9). p.2016-2023- Abstract
- Bacterial spores in a metabolically dormant state can survive long periods without nutrients under extreme environmental conditions. The molecular basis of spore dormancy is not well understood, but the distribution and physical state of water within the spore is thought to play an important role. Two scenarios have been proposed for the spore's core region, containing the DNA and most enzymes. In the gel scenario, the core is a structured macromolecular framework permeated by mobile water. In the glass scenario, the entire core, including the water, is an amorphous solid and the quenched molecular diffusion accounts for the spore's dormancy and thermal stability. Here, we use (2)H magnetic relaxation dispersion to selectively monitor... (More)
- Bacterial spores in a metabolically dormant state can survive long periods without nutrients under extreme environmental conditions. The molecular basis of spore dormancy is not well understood, but the distribution and physical state of water within the spore is thought to play an important role. Two scenarios have been proposed for the spore's core region, containing the DNA and most enzymes. In the gel scenario, the core is a structured macromolecular framework permeated by mobile water. In the glass scenario, the entire core, including the water, is an amorphous solid and the quenched molecular diffusion accounts for the spore's dormancy and thermal stability. Here, we use (2)H magnetic relaxation dispersion to selectively monitor water mobility in the core of Bacillus subtilis spores in the presence and absence of core Mn(2+) ions. We also report and analyze the solid-state (2)H NMR spectrum from these spores. Our NMR data clearly support the gel scenario with highly mobile core water (∼25 ps average rotational correlation time). Furthermore, we find that the large depot of manganese in the core is nearly anhydrous, with merely 1.7% on average of the maximum sixfold water coordination. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4179627
- author
- Kaieda, Shuji LU ; Setlow, Barbara ; Setlow, Peter and Halle, Bertil LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biophysical Journal
- volume
- 105
- issue
- 9
- pages
- 2016 - 2023
- publisher
- Cell Press
- external identifiers
-
- wos:000326858400011
- pmid:24209846
- scopus:84887332460
- ISSN
- 1542-0086
- DOI
- 10.1016/j.bpj.2013.09.022
- language
- English
- LU publication?
- yes
- id
- 6bdb4dc2-da82-411c-aa4f-38dff9ea74b8 (old id 4179627)
- date added to LUP
- 2016-04-01 10:59:07
- date last changed
- 2022-04-04 23:09:39
@article{6bdb4dc2-da82-411c-aa4f-38dff9ea74b8, abstract = {{Bacterial spores in a metabolically dormant state can survive long periods without nutrients under extreme environmental conditions. The molecular basis of spore dormancy is not well understood, but the distribution and physical state of water within the spore is thought to play an important role. Two scenarios have been proposed for the spore's core region, containing the DNA and most enzymes. In the gel scenario, the core is a structured macromolecular framework permeated by mobile water. In the glass scenario, the entire core, including the water, is an amorphous solid and the quenched molecular diffusion accounts for the spore's dormancy and thermal stability. Here, we use (2)H magnetic relaxation dispersion to selectively monitor water mobility in the core of Bacillus subtilis spores in the presence and absence of core Mn(2+) ions. We also report and analyze the solid-state (2)H NMR spectrum from these spores. Our NMR data clearly support the gel scenario with highly mobile core water (∼25 ps average rotational correlation time). Furthermore, we find that the large depot of manganese in the core is nearly anhydrous, with merely 1.7% on average of the maximum sixfold water coordination.}}, author = {{Kaieda, Shuji and Setlow, Barbara and Setlow, Peter and Halle, Bertil}}, issn = {{1542-0086}}, language = {{eng}}, number = {{9}}, pages = {{2016--2023}}, publisher = {{Cell Press}}, series = {{Biophysical Journal}}, title = {{Mobility of Core Water in Bacillus subtilis Spores by (2)H NMR.}}, url = {{http://dx.doi.org/10.1016/j.bpj.2013.09.022}}, doi = {{10.1016/j.bpj.2013.09.022}}, volume = {{105}}, year = {{2013}}, }