Electroporation Enhances the Metabolic Activity of Lactobacillus plantarum 564
Seratlic, Sanja ; Bugarski, Branko ; Radulovic, Zorica ; Dejmek, Petr LU
; Wadsö, Lars
LU
and Nedovic, Viktor
(2013)
In Food Technology and Biotechnology
51(4).
p.446-452
- Abstract
- The exposure of bacterial cells to pulsed electric fields (PEF) leads to the reversible formation of pores in the cell membrane if an applied energy is below the critical level. Therefore, the effect of electric field pulses with amplitudes below 14 kV/cm and the applied energy up to 12.2 J/cm(3) on the growth of Lactobacillus plantarum 564 cells was investigated. After PEF treatments, the growth of lactobacilli in De Man-Rogosa-Sharpe broth at 37 degrees C was monitored by isothermal calorimetry, absorbance and plate counts. All the applied treatments resulted in a higher growth rate of PEF-treated cells during early and mid-log phase, especially bacterial samples treated with lower field intensities (1.3-5.5 J/cm(3)). The transport of... (More)
- The exposure of bacterial cells to pulsed electric fields (PEF) leads to the reversible formation of pores in the cell membrane if an applied energy is below the critical level. Therefore, the effect of electric field pulses with amplitudes below 14 kV/cm and the applied energy up to 12.2 J/cm(3) on the growth of Lactobacillus plantarum 564 cells was investigated. After PEF treatments, the growth of lactobacilli in De Man-Rogosa-Sharpe broth at 37 degrees C was monitored by isothermal calorimetry, absorbance and plate counts. All the applied treatments resulted in a higher growth rate of PEF-treated cells during early and mid-log phase, especially bacterial samples treated with lower field intensities (1.3-5.5 J/cm(3)). The transport of ions and molecules through the cell membrane (which facilitates the growth of electroporated lactobacilli) was particularly evident in the mid-exponential growth phase, where the doubling time was reduced more than 3 times after the exposure to electric pulses of 5.5 Yore. The heat production rate during the growth of electroporated cells was also higher, indicating the enhanced metabolic activity of PEF-treated cells. Moreover, the electroporated cells had a better acidification ability than the untreated ones. It can be summarized that the applied PEF treatments with an energy input of below 12 J/cm(3) potentially induce reversible electroporation of the cell membrane, which has a positive impact on the growth and metabolic activity of the cells of lactobacilli. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4320141
- author
- Seratlic, Sanja
; Bugarski, Branko
; Radulovic, Zorica
; Dejmek, Petr
LU
; Wadsö, Lars
LU
and Nedovic, Viktor
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Lactobacillus plantarum, pulsed electric fields, isothermal calorimetry
- in
- Food Technology and Biotechnology
- volume
- 51
- issue
- 4
- pages
- 446 - 452
- publisher
- Faculty of Food Technology and Biotechnology University
- external identifiers
-
- wos:000329768300002
- scopus:84902368913
- ISSN
- 1330-9862
- language
- English
- LU publication?
- yes
- id
- 007729bd-f3a8-4b73-9d0a-e8c768c048a8 (old id 4320141)
- date added to LUP
- 2016-04-01 10:13:54
- date last changed
- 2023-11-09 15:24:26
@article{007729bd-f3a8-4b73-9d0a-e8c768c048a8,
abstract = {{The exposure of bacterial cells to pulsed electric fields (PEF) leads to the reversible formation of pores in the cell membrane if an applied energy is below the critical level. Therefore, the effect of electric field pulses with amplitudes below 14 kV/cm and the applied energy up to 12.2 J/cm(3) on the growth of Lactobacillus plantarum 564 cells was investigated. After PEF treatments, the growth of lactobacilli in De Man-Rogosa-Sharpe broth at 37 degrees C was monitored by isothermal calorimetry, absorbance and plate counts. All the applied treatments resulted in a higher growth rate of PEF-treated cells during early and mid-log phase, especially bacterial samples treated with lower field intensities (1.3-5.5 J/cm(3)). The transport of ions and molecules through the cell membrane (which facilitates the growth of electroporated lactobacilli) was particularly evident in the mid-exponential growth phase, where the doubling time was reduced more than 3 times after the exposure to electric pulses of 5.5 Yore. The heat production rate during the growth of electroporated cells was also higher, indicating the enhanced metabolic activity of PEF-treated cells. Moreover, the electroporated cells had a better acidification ability than the untreated ones. It can be summarized that the applied PEF treatments with an energy input of below 12 J/cm(3) potentially induce reversible electroporation of the cell membrane, which has a positive impact on the growth and metabolic activity of the cells of lactobacilli.}},
author = {{Seratlic, Sanja and Bugarski, Branko and Radulovic, Zorica and Dejmek, Petr and Wadsö, Lars and Nedovic, Viktor}},
issn = {{1330-9862}},
keywords = {{Lactobacillus plantarum; pulsed electric fields; isothermal calorimetry}},
language = {{eng}},
number = {{4}},
pages = {{446--452}},
publisher = {{Faculty of Food Technology and Biotechnology University}},
series = {{Food Technology and Biotechnology}},
title = {{Electroporation Enhances the Metabolic Activity of Lactobacillus plantarum 564}},
volume = {{51}},
year = {{2013}},
}