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Ultrahigh-pressure supercritical fluid extraction and chromatography of Moringa oleifera and Moringa peregrina seed lipids

Belo, Yannick Nuapia ; Al-Hamimi, Said LU ; Chimuka, Luke LU and Turner, Charlotta LU orcid (2019) In Analytical and Bioanalytical Chemistry 411(16). p.3685-3693
Abstract


An ultrahigh-pressure supercritical fluid extraction method was optimized and applied to extract seed oil lipids from two moringa species, namely Moringa oleifera (MO) and Moringa peregrina (MP). A full-factorial design was used to investigate the direct and interaction influence of pressure and temperature in the range of 40 to 80 MPa and 40 to 70 °C, respectively, on the extracted amount of oil from crushed seeds. The results revealed that pressure has a significant positive influence on the extracted amount of oil. The best extraction condition using neat CO
2
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An ultrahigh-pressure supercritical fluid extraction method was optimized and applied to extract seed oil lipids from two moringa species, namely Moringa oleifera (MO) and Moringa peregrina (MP). A full-factorial design was used to investigate the direct and interaction influence of pressure and temperature in the range of 40 to 80 MPa and 40 to 70 °C, respectively, on the extracted amount of oil from crushed seeds. The results revealed that pressure has a significant positive influence on the extracted amount of oil. The best extraction condition using neat CO
2
was found at 80 MPa and 57 °C, yielding 396 ± 23 and 529 ± 26 mg oil per gram of seeds for MO and MP, respectively. An extraction kinetics study revealed a mainly solubility-controlled extraction of oil, and 28 g of CO
2
was required to extract 400 mg of oil per gram of seeds of MO using the developed method. Addition of ethanol to the sample prior to the extraction increased the proportion of extractable polar lipids as well as the total amount of extracted oil. The developed method increased the extracted amount of oil twofold compared to a reference method based on solvent sonication. The obtained oil consisted mainly of glycerolipids, sterol esters, and phospholipids. Phospholipids, campesterol, and stigmasterol ester concentrations were found to be higher in MO while cholesterol ester was more abundant in MP.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Lipid profiling, Moringa oleifera, Moringa peregrina, Phospholipids, SFE, Sonication, Ultrahigh-pressure supercritical fluid extraction
in
Analytical and Bioanalytical Chemistry
volume
411
issue
16
pages
3685 - 3693
publisher
Springer
external identifiers
  • scopus:85065287383
  • pmid:31053955
ISSN
1618-2642
DOI
10.1007/s00216-019-01850-x
language
English
LU publication?
yes
id
447c4cfe-12db-4789-acc7-19e8e7298b08
date added to LUP
2019-05-24 12:02:19
date last changed
2024-06-25 14:47:58
@article{447c4cfe-12db-4789-acc7-19e8e7298b08,
  abstract     = {{<p><br>
                                                         An ultrahigh-pressure supercritical fluid extraction method was optimized and applied to extract seed oil lipids from two moringa species, namely Moringa oleifera (MO) and Moringa peregrina (MP). A full-factorial design was used to investigate the direct and interaction influence of pressure and temperature in the range of 40 to 80 MPa and 40 to 70 °C, respectively, on the extracted amount of oil from crushed seeds. The results revealed that pressure has a significant positive influence on the extracted amount of oil. The best extraction condition using neat CO                             <br>
                            <sub>2</sub><br>
                                                          was found at 80 MPa and 57 °C, yielding 396 ± 23 and 529 ± 26 mg oil per gram of seeds for MO and MP, respectively. An extraction kinetics study revealed a mainly solubility-controlled extraction of oil, and 28 g of CO                             <br>
                            <sub>2</sub><br>
                                                          was required to extract 400 mg of oil per gram of seeds of MO using the developed method. Addition of ethanol to the sample prior to the extraction increased the proportion of extractable polar lipids as well as the total amount of extracted oil. The developed method increased the extracted amount of oil twofold compared to a reference method based on solvent sonication. The obtained oil consisted mainly of glycerolipids, sterol esters, and phospholipids. Phospholipids, campesterol, and stigmasterol ester concentrations were found to be higher in MO while cholesterol ester was more abundant in MP.                         <br>
                        </p>}},
  author       = {{Belo, Yannick Nuapia and Al-Hamimi, Said and Chimuka, Luke and Turner, Charlotta}},
  issn         = {{1618-2642}},
  keywords     = {{Lipid profiling; Moringa oleifera; Moringa peregrina; Phospholipids; SFE; Sonication; Ultrahigh-pressure supercritical fluid extraction}},
  language     = {{eng}},
  number       = {{16}},
  pages        = {{3685--3693}},
  publisher    = {{Springer}},
  series       = {{Analytical and Bioanalytical Chemistry}},
  title        = {{Ultrahigh-pressure supercritical fluid extraction and chromatography of Moringa oleifera and Moringa peregrina seed lipids}},
  url          = {{http://dx.doi.org/10.1007/s00216-019-01850-x}},
  doi          = {{10.1007/s00216-019-01850-x}},
  volume       = {{411}},
  year         = {{2019}},
}