Controlled and uncontrolled asthma display distinct alveolar tissue matrix compositions
(2014) In Respiratory Research 15.- Abstract
- Objective: Whether distal inflammation in asthmatics also leads to structural changes in the alveolar parenchyma remains poorly examined, especially in patients with uncontrolled asthma. We hypothesized that patients who do not respond to conventional inhaled corticosteroid therapy have a distinct tissue composition, not only in central, but also in distal lung. Methods: Bronchial and transbronchial biopsies from healthy controls, patients with controlled atopic and patients with uncontrolled atopic asthma were processed for immunohistochemical analysis of fibroblasts and extracellular matrix molecules: collagen, versican, biglycan, decorin, fibronectin, EDA-fibronectin, matrix metalloproteinase (MMP)-9 and tissue-inhibitor of matrix... (More)
- Objective: Whether distal inflammation in asthmatics also leads to structural changes in the alveolar parenchyma remains poorly examined, especially in patients with uncontrolled asthma. We hypothesized that patients who do not respond to conventional inhaled corticosteroid therapy have a distinct tissue composition, not only in central, but also in distal lung. Methods: Bronchial and transbronchial biopsies from healthy controls, patients with controlled atopic and patients with uncontrolled atopic asthma were processed for immunohistochemical analysis of fibroblasts and extracellular matrix molecules: collagen, versican, biglycan, decorin, fibronectin, EDA-fibronectin, matrix metalloproteinase (MMP)-9 and tissue-inhibitor of matrix metalloproteinase (TIMP)-3. Results: In central airways we found increased percentage areas of versican and decorin in patients with uncontrolled asthma compared to both healthy controls and patients with controlled asthma. Percentage area of biglycan was significantly higher in both central airways and alveolar parenchyma of patients with uncontrolled compared to controlled asthma. Ratios of MMP-9/TIMP-3 were decreased in both uncontrolled and controlled asthma compared to healthy controls. In the alveolar parenchyma, patients with uncontrolled asthma had increased percentage areas of collagen, versican and decorin compared to patients with controlled asthma. Patients with uncontrolled asthma had significantly higher numbers of myofibroblasts in both central airways and alveolar parenchyma compared to patients with controlled asthma. Conclusions: Tissue composition differs, in both central and distal airways, between patients with uncontrolled and controlled asthma on equivalent doses of ICS. This altered structure and possible change in tissue elasticity may lead to abnormal mechanical properties, which could be a factor in the persistent symptoms for patients with uncontrolled asthma. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4602375
- author
- Weitoft, Maria LU ; Andersson, Cecilia LU ; Andersson Sjöland, Annika LU ; Tufvesson, Ellen LU ; Bjermer, Leif LU ; Erjefält, Jonas LU and Westergren-Thorsson, Gunilla LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Asthma, Controlled, Uncontrolled, ICS, Remodeling, Alveolar parenchyma, Extracellular matrix, Myofibroblasts
- in
- Respiratory Research
- volume
- 15
- article number
- 67
- publisher
- BioMed Central (BMC)
- external identifiers
-
- wos:000338670600001
- scopus:84902707564
- pmid:24950767
- ISSN
- 1465-9921
- DOI
- 10.1186/1465-9921-15-67
- language
- English
- LU publication?
- yes
- id
- 8e8f3276-39e5-4376-9dda-af96300811af (old id 4602375)
- date added to LUP
- 2016-04-01 10:37:16
- date last changed
- 2022-02-25 03:28:48
@article{8e8f3276-39e5-4376-9dda-af96300811af,
abstract = {{Objective: Whether distal inflammation in asthmatics also leads to structural changes in the alveolar parenchyma remains poorly examined, especially in patients with uncontrolled asthma. We hypothesized that patients who do not respond to conventional inhaled corticosteroid therapy have a distinct tissue composition, not only in central, but also in distal lung. Methods: Bronchial and transbronchial biopsies from healthy controls, patients with controlled atopic and patients with uncontrolled atopic asthma were processed for immunohistochemical analysis of fibroblasts and extracellular matrix molecules: collagen, versican, biglycan, decorin, fibronectin, EDA-fibronectin, matrix metalloproteinase (MMP)-9 and tissue-inhibitor of matrix metalloproteinase (TIMP)-3. Results: In central airways we found increased percentage areas of versican and decorin in patients with uncontrolled asthma compared to both healthy controls and patients with controlled asthma. Percentage area of biglycan was significantly higher in both central airways and alveolar parenchyma of patients with uncontrolled compared to controlled asthma. Ratios of MMP-9/TIMP-3 were decreased in both uncontrolled and controlled asthma compared to healthy controls. In the alveolar parenchyma, patients with uncontrolled asthma had increased percentage areas of collagen, versican and decorin compared to patients with controlled asthma. Patients with uncontrolled asthma had significantly higher numbers of myofibroblasts in both central airways and alveolar parenchyma compared to patients with controlled asthma. Conclusions: Tissue composition differs, in both central and distal airways, between patients with uncontrolled and controlled asthma on equivalent doses of ICS. This altered structure and possible change in tissue elasticity may lead to abnormal mechanical properties, which could be a factor in the persistent symptoms for patients with uncontrolled asthma.}},
author = {{Weitoft, Maria and Andersson, Cecilia and Andersson Sjöland, Annika and Tufvesson, Ellen and Bjermer, Leif and Erjefält, Jonas and Westergren-Thorsson, Gunilla}},
issn = {{1465-9921}},
keywords = {{Asthma; Controlled; Uncontrolled; ICS; Remodeling; Alveolar parenchyma; Extracellular matrix; Myofibroblasts}},
language = {{eng}},
publisher = {{BioMed Central (BMC)}},
series = {{Respiratory Research}},
title = {{Controlled and uncontrolled asthma display distinct alveolar tissue matrix compositions}},
url = {{https://lup.lub.lu.se/search/files/1996531/7864150}},
doi = {{10.1186/1465-9921-15-67}},
volume = {{15}},
year = {{2014}},
}