Link N is Cleaved by Human Annulus Fibrosus Cells Generating a Fragment With Retained Biological Activity
(2014) In Journal of Orthopaedic Research 32(9). p.1189-1197- Abstract
- Presently, there are no established treatments to prevent, stop or even retard back pain arising from disc degeneration. Previous studies have shown that Link N can act as a growth factor and stimulate the synthesis of proteoglycans and collagens, in IVD. However, the sequences in Link N involved in modulating cellular activity are not well understood. To determine if disc cells can proteolytically process Link N, human disc cells were exposed to native Link N over a 48 h period and mass spectrometric analysis revealed that a peptide spanning residues 1-8 was generated in the presence of AF cells but not NP cells. Link N 1-8 significantly induced proteoglycan production in the presence of IL-1 beta NP and AF cells, confirming that the... (More)
- Presently, there are no established treatments to prevent, stop or even retard back pain arising from disc degeneration. Previous studies have shown that Link N can act as a growth factor and stimulate the synthesis of proteoglycans and collagens, in IVD. However, the sequences in Link N involved in modulating cellular activity are not well understood. To determine if disc cells can proteolytically process Link N, human disc cells were exposed to native Link N over a 48 h period and mass spectrometric analysis revealed that a peptide spanning residues 1-8 was generated in the presence of AF cells but not NP cells. Link N 1-8 significantly induced proteoglycan production in the presence of IL-1 beta NP and AF cells, confirming that the biological effect is maintained in the first 8 amino acids of the peptide and indicating that the effect is sustained in an inflammatory environment. Thus Link-N 1-8 could be a promising candidate for biologically induced disc repair, and the identification of such a stable specific peptide may facilitate the design of compounds to promote disc repair and provide alternatives to surgical intervention for early stage disc degeneration. (C) 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4648925
- author
- Gawri, Rahul ; Ouellet, Jean ; Onnerfjord, Patrik ; Alkhatib, Bashar ; Steffen, Thomas ; Heinegård, Dick LU ; Roughley, Peter ; Antoniou, John ; Mwale, Fackson and Haglund, Lisbet
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- IVD degeneration and regeneration, Link N, organ culture, biological, repair
- in
- Journal of Orthopaedic Research
- volume
- 32
- issue
- 9
- pages
- 1189 - 1197
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000340587200015
- scopus:84904422404
- pmid:24861010
- ISSN
- 1554-527X
- DOI
- 10.1002/jor.22653
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Connective Tissue Biology (013230151)
- id
- 823f4553-7d74-4b21-b6ba-cf922ebfbf0a (old id 4648925)
- date added to LUP
- 2016-04-01 11:06:15
- date last changed
- 2022-01-26 05:22:07
@article{823f4553-7d74-4b21-b6ba-cf922ebfbf0a,
abstract = {{Presently, there are no established treatments to prevent, stop or even retard back pain arising from disc degeneration. Previous studies have shown that Link N can act as a growth factor and stimulate the synthesis of proteoglycans and collagens, in IVD. However, the sequences in Link N involved in modulating cellular activity are not well understood. To determine if disc cells can proteolytically process Link N, human disc cells were exposed to native Link N over a 48 h period and mass spectrometric analysis revealed that a peptide spanning residues 1-8 was generated in the presence of AF cells but not NP cells. Link N 1-8 significantly induced proteoglycan production in the presence of IL-1 beta NP and AF cells, confirming that the biological effect is maintained in the first 8 amino acids of the peptide and indicating that the effect is sustained in an inflammatory environment. Thus Link-N 1-8 could be a promising candidate for biologically induced disc repair, and the identification of such a stable specific peptide may facilitate the design of compounds to promote disc repair and provide alternatives to surgical intervention for early stage disc degeneration. (C) 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.}},
author = {{Gawri, Rahul and Ouellet, Jean and Onnerfjord, Patrik and Alkhatib, Bashar and Steffen, Thomas and Heinegård, Dick and Roughley, Peter and Antoniou, John and Mwale, Fackson and Haglund, Lisbet}},
issn = {{1554-527X}},
keywords = {{IVD degeneration and regeneration; Link N; organ culture; biological; repair}},
language = {{eng}},
number = {{9}},
pages = {{1189--1197}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Orthopaedic Research}},
title = {{Link N is Cleaved by Human Annulus Fibrosus Cells Generating a Fragment With Retained Biological Activity}},
url = {{http://dx.doi.org/10.1002/jor.22653}},
doi = {{10.1002/jor.22653}},
volume = {{32}},
year = {{2014}},
}