A sustainable approach for the extraction of cholesterol-lowering compounds from an olive by-product based on CO2-expanded ethyl acetate
Vásquez-Villanueva, Romy LU ; Plaza, Merichel LU ; García, María Concepción ; Turner, Charlotta LU
and Marina, María Luisa
(2019)
In Analytical and Bioanalytical Chemistry
411(22).
p.5885-5896
- Abstract
Olive (Olea europaea) processing results in large amounts of by-products that contain valuable molecules such as phenolic compounds and phytosterols. These molecules have demonstrated to reduce blood cholesterol levels. This work proposes the development of a method to obtain simultaneously phenolic compounds and phytosterols from the olive stone using CO2-expanded liquid extraction. Hansen solubility parameters were employed for the theoretical prediction of the most suitable bio-based solvent to extract target compounds. The Box–Behnken experimental design was employed to select the optimal conditions of pressure (8–25 MPa), the molar fraction of CO2 in ethyl acetate (0.15–0.55), and the temperature (40–80 °C).... (More)
Olive (Olea europaea) processing results in large amounts of by-products that contain valuable molecules such as phenolic compounds and phytosterols. These molecules have demonstrated to reduce blood cholesterol levels. This work proposes the development of a method to obtain simultaneously phenolic compounds and phytosterols from the olive stone using CO2-expanded liquid extraction. Hansen solubility parameters were employed for the theoretical prediction of the most suitable bio-based solvent to extract target compounds. The Box–Behnken experimental design was employed to select the optimal conditions of pressure (8–25 MPa), the molar fraction of CO2 in ethyl acetate (0.15–0.55), and the temperature (40–80 °C). Extracts showing the highest and the lowest reductions of micellar cholesterol solubility capacity were analyzed by gas chromatography coupled to mass spectrometry to find out the compounds responsible for this activity. Different phenolic compounds, free fatty acids, and phytosterols were identified in the extracts. β-Sitosterol and, especially, tyrosol and hydroxytyrosol were the compounds that primarily contributed to the reduction of micellar cholesterol solubility capacity.
(Less)
- author
- Vásquez-Villanueva, Romy
LU
; Plaza, Merichel
LU
; García, María Concepción
; Turner, Charlotta
LU
and Marina, María Luisa
- organization
- publishing date
- 2019-07-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bio-based solvents, Cholesterol-lowering capacity, CO-expanded liquid, Hansen solubility parameters, Olive seed, Phenolic compounds, Phytosterols
- in
- Analytical and Bioanalytical Chemistry
- volume
- 411
- issue
- 22
- pages
- 5885 - 5896
- publisher
- Springer
- external identifiers
-
- scopus:85068846508
- pmid:31280476
- ISSN
- 1618-2642
- DOI
- 10.1007/s00216-019-01970-4
- language
- English
- LU publication?
- yes
- id
- 47e3c18d-7eb1-4f3b-a7a7-d46c301107bc
- date added to LUP
- 2019-07-25 08:00:42
- date last changed
- 2024-06-13 01:03:27
@article{47e3c18d-7eb1-4f3b-a7a7-d46c301107bc,
abstract = {{<p>Olive (Olea europaea) processing results in large amounts of by-products that contain valuable molecules such as phenolic compounds and phytosterols. These molecules have demonstrated to reduce blood cholesterol levels. This work proposes the development of a method to obtain simultaneously phenolic compounds and phytosterols from the olive stone using CO<sub>2</sub>-expanded liquid extraction. Hansen solubility parameters were employed for the theoretical prediction of the most suitable bio-based solvent to extract target compounds. The Box–Behnken experimental design was employed to select the optimal conditions of pressure (8–25 MPa), the molar fraction of CO<sub>2</sub> in ethyl acetate (0.15–0.55), and the temperature (40–80 °C). Extracts showing the highest and the lowest reductions of micellar cholesterol solubility capacity were analyzed by gas chromatography coupled to mass spectrometry to find out the compounds responsible for this activity. Different phenolic compounds, free fatty acids, and phytosterols were identified in the extracts. β-Sitosterol and, especially, tyrosol and hydroxytyrosol were the compounds that primarily contributed to the reduction of micellar cholesterol solubility capacity.</p>}},
author = {{Vásquez-Villanueva, Romy and Plaza, Merichel and García, María Concepción and Turner, Charlotta and Marina, María Luisa}},
issn = {{1618-2642}},
keywords = {{Bio-based solvents; Cholesterol-lowering capacity; CO-expanded liquid; Hansen solubility parameters; Olive seed; Phenolic compounds; Phytosterols}},
language = {{eng}},
month = {{07}},
number = {{22}},
pages = {{5885--5896}},
publisher = {{Springer}},
series = {{Analytical and Bioanalytical Chemistry}},
title = {{A sustainable approach for the extraction of cholesterol-lowering compounds from an olive by-product based on CO<sub>2</sub>-expanded ethyl acetate}},
url = {{http://dx.doi.org/10.1007/s00216-019-01970-4}},
doi = {{10.1007/s00216-019-01970-4}},
volume = {{411}},
year = {{2019}},
}