Protein Microgels from Amyloid Fibril Networks
(2015) In ACS Nano 9(1). p.43-51- Abstract
- Nanofibrillar forms of proteins were initially recognized in the context of pathology, but more recently have been discovered in a range of functional roles in nature, including as active catalytic scaffolds and bacterial coatings. Here we show that protein nanofibrils can be used to form the basis of monodisperse microgels and gel shells composed of naturally occurring proteins. We explore the potential of these protein microgels to act as drug carrier agents, and demonstrate the controlled release of four different encapsulated drug-like small molecules, as well as the component proteins themselves. Furthermore, we show that protein nanofibril self-assembly can continue after the initial formation of the microgel particles, and that this... (More)
- Nanofibrillar forms of proteins were initially recognized in the context of pathology, but more recently have been discovered in a range of functional roles in nature, including as active catalytic scaffolds and bacterial coatings. Here we show that protein nanofibrils can be used to form the basis of monodisperse microgels and gel shells composed of naturally occurring proteins. We explore the potential of these protein microgels to act as drug carrier agents, and demonstrate the controlled release of four different encapsulated drug-like small molecules, as well as the component proteins themselves. Furthermore, we show that protein nanofibril self-assembly can continue after the initial formation of the microgel particles, and that this process results in active materials with network densities that can be modulated in situ. We demonstrate that these materials are nontoxic to human cells and that they can be used to enhance the efficacy of antibiotics relative to delivery in homogeneous solution. Because of the biocompatibility and biodegradability of natural proteins used in the fabrication of the microgels, as well as their ability to control the release of small molecules and biopolymers, protein nanofibril microgels represent a promising class of functional artificial multiscale materials generated from natural building blocks. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5179960
- author
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- microfluidics, protein nanofibrils, microgels, lysozyme, drug release
- in
- ACS Nano
- volume
- 9
- issue
- 1
- pages
- 43 - 51
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000348619000006
- scopus:84921773788
- pmid:25469621
- ISSN
- 1936-086X
- DOI
- 10.1021/nn504869d
- language
- English
- LU publication?
- yes
- id
- 4d2d427b-98fc-4150-9635-a1f01fce2517 (old id 5179960)
- date added to LUP
- 2016-04-01 10:26:29
- date last changed
- 2022-04-27 21:57:23
@article{4d2d427b-98fc-4150-9635-a1f01fce2517,
abstract = {{Nanofibrillar forms of proteins were initially recognized in the context of pathology, but more recently have been discovered in a range of functional roles in nature, including as active catalytic scaffolds and bacterial coatings. Here we show that protein nanofibrils can be used to form the basis of monodisperse microgels and gel shells composed of naturally occurring proteins. We explore the potential of these protein microgels to act as drug carrier agents, and demonstrate the controlled release of four different encapsulated drug-like small molecules, as well as the component proteins themselves. Furthermore, we show that protein nanofibril self-assembly can continue after the initial formation of the microgel particles, and that this process results in active materials with network densities that can be modulated in situ. We demonstrate that these materials are nontoxic to human cells and that they can be used to enhance the efficacy of antibiotics relative to delivery in homogeneous solution. Because of the biocompatibility and biodegradability of natural proteins used in the fabrication of the microgels, as well as their ability to control the release of small molecules and biopolymers, protein nanofibril microgels represent a promising class of functional artificial multiscale materials generated from natural building blocks.}},
author = {{Shimanovich, Ulyana and Efimov, Igor and Mason, Thomas O. and Flagmeier, Patrick and Buell, Alexander K. and Gedanken, Aharon and Linse, Sara and Akerfeldt, Karin S. and Dobson, Christopher M. and Weitz, David A. and Knowles, Tuomas P. J.}},
issn = {{1936-086X}},
keywords = {{microfluidics; protein nanofibrils; microgels; lysozyme; drug release}},
language = {{eng}},
number = {{1}},
pages = {{43--51}},
publisher = {{The American Chemical Society (ACS)}},
series = {{ACS Nano}},
title = {{Protein Microgels from Amyloid Fibril Networks}},
url = {{http://dx.doi.org/10.1021/nn504869d}},
doi = {{10.1021/nn504869d}},
volume = {{9}},
year = {{2015}},
}