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Critical role of P-selectin-dependent leukocyte recruitment in endotoxin-induced intestinal barrier dysfunction in mice.

Mangell, Peter LU ; Röme, Andrada LU ; Wang, Yusheng LU ; Schramm, R ; Jeppsson, Bengt LU and Thorlacius, Henrik LU (2007) In Inflammation Research 56(5). p.189-194
Abstract
Objective: To define the importance of leukocyte recruitment in endotoxin-induced gut permeability. Materials and methods: 31 male C57BL/6 mice were challenged with lipopolysaccharide (LPS). Ileal permeability was measured in Ussing chambers and leukocyte-endothelium interactions studied with intravital fluorescence microscopy after 18 h. Results: LPS caused a clear-cut increase in leukocyte accumulation and intestinal permeability. Immunoneutralisation of P-selectin not only reduced leukocyte recruitment significantly (54% reduction) but also abolished endotoxin-induced intestinal leakage. Intestinal levels of pro-inflammatory chemokines increased markedly in response to LPS but were not influenced by inhibition of P-selectin in vivo.... (More)
Objective: To define the importance of leukocyte recruitment in endotoxin-induced gut permeability. Materials and methods: 31 male C57BL/6 mice were challenged with lipopolysaccharide (LPS). Ileal permeability was measured in Ussing chambers and leukocyte-endothelium interactions studied with intravital fluorescence microscopy after 18 h. Results: LPS caused a clear-cut increase in leukocyte accumulation and intestinal permeability. Immunoneutralisation of P-selectin not only reduced leukocyte recruitment significantly (54% reduction) but also abolished endotoxin-induced intestinal leakage. Intestinal levels of pro-inflammatory chemokines increased markedly in response to LPS but were not influenced by inhibition of P-selectin in vivo. Conclusion: The present study shows not only that endotoxin-induced leukocyte recruitment is mediated by P-selectin but also that sepsis-associated intestinal leakage in the gut is largely regulated by leukocyte accumulation. Thus, our novel data demonstrate a critical link between P-selectin-dependent leukocyte recruitment and gut barrier failure in endotoxemia. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
P-selectin, leukocyte, sepsis, intestinal barrier, endotoxin
in
Inflammation Research
volume
56
issue
5
pages
189 - 194
publisher
Birkhäuser Verlag
external identifiers
  • wos:000246804400003
  • scopus:34250890739
ISSN
1420-908X
DOI
10.1007/s00011-007-6163-x
language
English
LU publication?
yes
id
175813ce-128b-4ae9-baa9-1d2595b0d121 (old id 539596)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17588134&dopt=Abstract
date added to LUP
2016-04-01 11:48:40
date last changed
2022-01-26 18:35:43
@article{175813ce-128b-4ae9-baa9-1d2595b0d121,
  abstract     = {{Objective: To define the importance of leukocyte recruitment in endotoxin-induced gut permeability. Materials and methods: 31 male C57BL/6 mice were challenged with lipopolysaccharide (LPS). Ileal permeability was measured in Ussing chambers and leukocyte-endothelium interactions studied with intravital fluorescence microscopy after 18 h. Results: LPS caused a clear-cut increase in leukocyte accumulation and intestinal permeability. Immunoneutralisation of P-selectin not only reduced leukocyte recruitment significantly (54% reduction) but also abolished endotoxin-induced intestinal leakage. Intestinal levels of pro-inflammatory chemokines increased markedly in response to LPS but were not influenced by inhibition of P-selectin in vivo. Conclusion: The present study shows not only that endotoxin-induced leukocyte recruitment is mediated by P-selectin but also that sepsis-associated intestinal leakage in the gut is largely regulated by leukocyte accumulation. Thus, our novel data demonstrate a critical link between P-selectin-dependent leukocyte recruitment and gut barrier failure in endotoxemia.}},
  author       = {{Mangell, Peter and Röme, Andrada and Wang, Yusheng and Schramm, R and Jeppsson, Bengt and Thorlacius, Henrik}},
  issn         = {{1420-908X}},
  keywords     = {{P-selectin; leukocyte; sepsis; intestinal barrier; endotoxin}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{189--194}},
  publisher    = {{Birkhäuser Verlag}},
  series       = {{Inflammation Research}},
  title        = {{Critical role of P-selectin-dependent leukocyte recruitment in endotoxin-induced intestinal barrier dysfunction in mice.}},
  url          = {{https://lup.lub.lu.se/search/files/2652337/626008.pdf}},
  doi          = {{10.1007/s00011-007-6163-x}},
  volume       = {{56}},
  year         = {{2007}},
}