Trisomies in Hematologic Malignancies
(2005) In Lund University Faculty of Medicine Doctoral Dissertation Series- Abstract
- Acquired clonal chromosome aberrations, closely associated with leukemogenesis, are found in many hematologic malignancies. Although the balanced rearrangements, such as translocations and inversions, are the ones most commonly thought of in the context of leukemias, gains of chromosomes ? e.g.., trisomies ? are also frequent. Little is known about the biologic outcome, the pathogenetic significance, or the mechanism behind the formation of the latter genetic changes. The aim of the present thesis was to address these issues, focusing on trisomy 8 as the sole cytogenetic change in acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS), and on high hyperdiploidy ? gains of multiple chromosomes ? in childhood acute lymphoblastic... (More)
- Acquired clonal chromosome aberrations, closely associated with leukemogenesis, are found in many hematologic malignancies. Although the balanced rearrangements, such as translocations and inversions, are the ones most commonly thought of in the context of leukemias, gains of chromosomes ? e.g.., trisomies ? are also frequent. Little is known about the biologic outcome, the pathogenetic significance, or the mechanism behind the formation of the latter genetic changes. The aim of the present thesis was to address these issues, focusing on trisomy 8 as the sole cytogenetic change in acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS), and on high hyperdiploidy ? gains of multiple chromosomes ? in childhood acute lymphoblastic leukemia (ALL). In the first two articles, AML and MDS cases with +8 as the seemingly sole abnormality were studied with fluorescence in situ hybridization (FISH) analysis and high-resolution genome-wide array-based comparative genome hybridization (array CGH), respectively, in order to determine whether this chromosome abnormality was truly isolated or if there were additional, cytogenetically cryptic, anomalies present. Whereas multicolor-FISH, FISH with partial chromosome paint and subtelomeric probes for 8p and 8q, and FISH with probes specific for four leukemia-associated chromosome 8 genes failed to reveal any hidden genetic changes, array CGH identified twelve previously undetected imbalances, involving 0.2 ? 4.9 Mb and many genes, of putative importance in the leukemogenesis. Thus, it was shown that trisomy 8 is not always the primary genetic event, even when it is the sole anomaly seen with standard cytogenetic methods. In articles III and IV, the formation of hyperdiploidy and the possibility of imprinting effects related to the parental origin of the chromosomes gained in this cytogenetic subgroup were investigated. Combining the results from the two studies, it could, for the first time, be demonstrated that hyperdiploidy most frequently arises by a simultaneous gain of chromosomes in a single abnormal cell division, with a subset of cases occurring by other pathways. Furthermore, preferential duplication of maternal or paternal homologues was not seen for any of the commonly gained chromosomes in these investigations. Hence, imprinting effects resulting from such skewness could be excluded as the pathogenetically important outcome of the extra chromosomes in hyperdiploid childhood ALL. In summary, the results presented in this thesis have extended our knowledge of trisomies in malignant hematologic disorders and increased the understanding of their significance in the leukemogenic processes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/545407
- author
- Paulsson, Kajsa LU
- supervisor
- opponent
-
- Professor Harrison, Christine J., Cancer Sciences Division, Southampton General Hospital, UK
- organization
- publishing date
- 2005
- type
- Thesis
- publication status
- published
- subject
- keywords
- Clinical genetics, Klinisk genetik, cytogenetik, Genetik, cytogenetics, Genetics, leukemia, chromosome aberration, trisomy
- in
- Lund University Faculty of Medicine Doctoral Dissertation Series
- pages
- 112 pages
- publisher
- Section of Clinical Genetics, Institution of Laboratory Medicine, Lund University Hospital
- defense location
- Föreläsningssal F3, centralblocket, Universitetessjukhuset i Lund
- defense date
- 2005-10-07 10:00:00
- ISSN
- 1652-8220
- ISBN
- 91-85439-82-7
- language
- English
- LU publication?
- yes
- additional info
- K Paulsson, T Fioretos, B Strömbeck, N Mauritzson, HJ Tanke and B Johansson. 2003. Trisomy 8 as the sole chromosomal aberration in myelocytic malignancies: a multicolor and locusspecific fluorescence in situ hybridization study. Cancer Genet Cytogenet, vol 140 pp 66-69.K Paulsson, M Heidenblad, B Strömbeck, J Staaf, G Jönsson, Å Borg, T Fioretos and B Johansson. . High-resolution genomewide array-based comparative genomehybridization reveals cryptic chromosome changes in AML and MDS cases with trisomy 8 as the sole cytogenetic aberration. (manuscript)K Paulsson, I Panagopoulos, S Knuutila, KJ Jee, S Garwicz, T Fioretos, F MItelman and B Johansson. 2003. Formation of trisomies and their parental origin in hyperdiploid childhood acute lymphoblastic leukemia. Blood, vol 102 pp 3010-3015.K Paulsson, H Mörse, T Fioretos, M Behrendtz, B Strömbeck and B Johansson. 2005. Evidence for a single-step mechanism in the origin of hyperdiploid childhood acute lymphoblastic leukemia. Genes Chromosomes Cancer, vol 44 pp 113-122.
- id
- 4dfcdcd5-64d6-4da1-b7ab-37ca4729d893 (old id 545407)
- date added to LUP
- 2016-04-01 16:01:15
- date last changed
- 2019-05-21 21:47:10
@phdthesis{4dfcdcd5-64d6-4da1-b7ab-37ca4729d893, abstract = {{Acquired clonal chromosome aberrations, closely associated with leukemogenesis, are found in many hematologic malignancies. Although the balanced rearrangements, such as translocations and inversions, are the ones most commonly thought of in the context of leukemias, gains of chromosomes ? e.g.., trisomies ? are also frequent. Little is known about the biologic outcome, the pathogenetic significance, or the mechanism behind the formation of the latter genetic changes. The aim of the present thesis was to address these issues, focusing on trisomy 8 as the sole cytogenetic change in acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS), and on high hyperdiploidy ? gains of multiple chromosomes ? in childhood acute lymphoblastic leukemia (ALL). In the first two articles, AML and MDS cases with +8 as the seemingly sole abnormality were studied with fluorescence in situ hybridization (FISH) analysis and high-resolution genome-wide array-based comparative genome hybridization (array CGH), respectively, in order to determine whether this chromosome abnormality was truly isolated or if there were additional, cytogenetically cryptic, anomalies present. Whereas multicolor-FISH, FISH with partial chromosome paint and subtelomeric probes for 8p and 8q, and FISH with probes specific for four leukemia-associated chromosome 8 genes failed to reveal any hidden genetic changes, array CGH identified twelve previously undetected imbalances, involving 0.2 ? 4.9 Mb and many genes, of putative importance in the leukemogenesis. Thus, it was shown that trisomy 8 is not always the primary genetic event, even when it is the sole anomaly seen with standard cytogenetic methods. In articles III and IV, the formation of hyperdiploidy and the possibility of imprinting effects related to the parental origin of the chromosomes gained in this cytogenetic subgroup were investigated. Combining the results from the two studies, it could, for the first time, be demonstrated that hyperdiploidy most frequently arises by a simultaneous gain of chromosomes in a single abnormal cell division, with a subset of cases occurring by other pathways. Furthermore, preferential duplication of maternal or paternal homologues was not seen for any of the commonly gained chromosomes in these investigations. Hence, imprinting effects resulting from such skewness could be excluded as the pathogenetically important outcome of the extra chromosomes in hyperdiploid childhood ALL. In summary, the results presented in this thesis have extended our knowledge of trisomies in malignant hematologic disorders and increased the understanding of their significance in the leukemogenic processes.}}, author = {{Paulsson, Kajsa}}, isbn = {{91-85439-82-7}}, issn = {{1652-8220}}, keywords = {{Clinical genetics; Klinisk genetik; cytogenetik; Genetik; cytogenetics; Genetics; leukemia; chromosome aberration; trisomy}}, language = {{eng}}, publisher = {{Section of Clinical Genetics, Institution of Laboratory Medicine, Lund University Hospital}}, school = {{Lund University}}, series = {{Lund University Faculty of Medicine Doctoral Dissertation Series}}, title = {{Trisomies in Hematologic Malignancies}}, url = {{https://lup.lub.lu.se/search/files/4545032/545408.pdf}}, year = {{2005}}, }