Activation of immune responses against the basement membrane component collagen type IV does not affect the development of atherosclerosis in ApoE-deficient mice
(2019) In Scientific Reports 9(1).- Abstract
Oxidation of low-density lipoprotein (LDL) in the arterial extracellular matrix results in malondialdehyde (MDA)-modifications of surrounding matrix proteins. We have recently demonstrated an association between high levels of autoantibodies against MDA-modified collagen type IV and risk for development of myocardial infarction. Collagen type IV is an important component of the endothelial basement membrane and influences smooth muscle cell function. We hypothesized that immune responses against collagen type IV could contribute to vascular injury affecting the development of atherosclerosis. To investigate this possibility, we induced an antibody-response against collagen... (More)
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Oxidation of low-density lipoprotein (LDL) in the arterial extracellular matrix results in malondialdehyde (MDA)-modifications of surrounding matrix proteins. We have recently demonstrated an association between high levels of autoantibodies against MDA-modified collagen type IV and risk for development of myocardial infarction. Collagen type IV is an important component of the endothelial basement membrane and influences smooth muscle cell function. We hypothesized that immune responses against collagen type IV could contribute to vascular injury affecting the development of atherosclerosis. To investigate this possibility, we induced an antibody-response against collagen type IV in apolipoprotein E (Apo E)-deficient mice. Female ApoE
−/−
mice on C57BL/6 background were immunized with α1α2 type IV collagen chain peptides linked to the immune-enhancer PADRE, PADRE alone or PBS at 12 weeks of age with three subsequent booster injections before the mice were killed at 23 weeks of age. Immunization of PADRE alone induced autoantibodies against PADRE, increased IL-4 secretion from splenocytes and reduced SMC content in the subvalvular plaques. Immunization with peptides of α1α2 type IV collagen chains induced a strong IgG1antibody response against collagen type IV peptides without affecting the distribution of T cell populations, plasma cytokine or lipid levels. There were no differences in atherosclerotic plaque development between collagen α1α2(IV)-PADRE immunized mice and control mice. Our findings demonstrate that the presence of antibodies against the basement membrane component collagen type IV does not affect atherosclerosis development in ApoE
−/−
mice. This suggests that the association between autoantibodies against collagen type IV and risk for myocardial infarction found in humans does not reflect a pathogenic role of these autoantibodies.
- author
- Vallejo, J. LU ; Dunér, P. LU ; To, F. LU ; Engelbertsen, D. LU ; Gonçalves, I. LU ; Nilsson, J. LU and Bengtsson, E. LU
- organization
-
- Cardiovascular Research - Matrix and Inflammation in Atherosclerosis (research group)
- EXODIAB: Excellence of Diabetes Research in Sweden
- Cardiovascular Research - Cellular Metabolism and Inflammation (research group)
- Cardiovascular Research - Translational Studies (research group)
- Cardiovascular Research - Immunity and Atherosclerosis (research group)
- EpiHealth: Epidemiology for Health
- publishing date
- 2019-04-12
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 9
- issue
- 1
- article number
- 5964
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:30979943
- scopus:85064349989
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-019-42375-8
- language
- English
- LU publication?
- yes
- id
- 598f5daf-7e20-4e10-b60d-c1c177db127e
- date added to LUP
- 2019-05-02 13:08:32
- date last changed
- 2024-02-14 22:50:49
@article{598f5daf-7e20-4e10-b60d-c1c177db127e, abstract = {{<p><br> Oxidation of low-density lipoprotein (LDL) in the arterial extracellular matrix results in malondialdehyde (MDA)-modifications of surrounding matrix proteins. We have recently demonstrated an association between high levels of autoantibodies against MDA-modified collagen type IV and risk for development of myocardial infarction. Collagen type IV is an important component of the endothelial basement membrane and influences smooth muscle cell function. We hypothesized that immune responses against collagen type IV could contribute to vascular injury affecting the development of atherosclerosis. To investigate this possibility, we induced an antibody-response against collagen type IV in apolipoprotein E (Apo E)-deficient mice. Female ApoE <br> <sup>−/−</sup><br> mice on C57BL/6 background were immunized with α1α2 type IV collagen chain peptides linked to the immune-enhancer PADRE, PADRE alone or PBS at 12 weeks of age with three subsequent booster injections before the mice were killed at 23 weeks of age. Immunization of PADRE alone induced autoantibodies against PADRE, increased IL-4 secretion from splenocytes and reduced SMC content in the subvalvular plaques. Immunization with peptides of α1α2 type IV collagen chains induced a strong IgG1antibody response against collagen type IV peptides without affecting the distribution of T cell populations, plasma cytokine or lipid levels. There were no differences in atherosclerotic plaque development between collagen α1α2(IV)-PADRE immunized mice and control mice. Our findings demonstrate that the presence of antibodies against the basement membrane component collagen type IV does not affect atherosclerosis development in ApoE <br> <sup>−/−</sup><br> mice. This suggests that the association between autoantibodies against collagen type IV and risk for myocardial infarction found in humans does not reflect a pathogenic role of these autoantibodies. <br> </p>}}, author = {{Vallejo, J. and Dunér, P. and To, F. and Engelbertsen, D. and Gonçalves, I. and Nilsson, J. and Bengtsson, E.}}, issn = {{2045-2322}}, language = {{eng}}, month = {{04}}, number = {{1}}, publisher = {{Nature Publishing Group}}, series = {{Scientific Reports}}, title = {{Activation of immune responses against the basement membrane component collagen type IV does not affect the development of atherosclerosis in ApoE-deficient mice}}, url = {{http://dx.doi.org/10.1038/s41598-019-42375-8}}, doi = {{10.1038/s41598-019-42375-8}}, volume = {{9}}, year = {{2019}}, }