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Optimizing clinical O2 saturation mapping using hyperspectral imaging and diffuse reflectance spectroscopy in the context of epinephrine injection

Gustafsson, Nils LU ; Bunke, Josefine LU ; Magnusson, Ludvig ; Albinsson, John LU orcid ; Hérnandez-Palacios, Julio ; Sheikh, Rafi LU orcid ; Malmsjö, Malin LU orcid and Merdasa, Aboma LU orcid (2024) In Biomed. Opt. Express 15(3). p.1995-2013
Abstract
Clinical determination of oxygen saturation (sO2) in patients is commonly performed via non-invasive optical techniques. However, reliance on a few wavelengths and some form of pre-determined calibration introduces limits to how these methods can be used. One example involves the assessment of sO2 after injection of local anesthetic using epinephrine, where some controversy exists around the time it takes for the epinephrine to have an effect. This is likely caused by a change in the tissue environment not accounted for by standard calibrated instruments and conventional analysis techniques. The present study aims to account for this changing environment by acquiring absorption spectra using hyperspectral imaging (HSI) and diffuse... (More)
Clinical determination of oxygen saturation (sO2) in patients is commonly performed via non-invasive optical techniques. However, reliance on a few wavelengths and some form of pre-determined calibration introduces limits to how these methods can be used. One example involves the assessment of sO2 after injection of local anesthetic using epinephrine, where some controversy exists around the time it takes for the epinephrine to have an effect. This is likely caused by a change in the tissue environment not accounted for by standard calibrated instruments and conventional analysis techniques. The present study aims to account for this changing environment by acquiring absorption spectra using hyperspectral imaging (HSI) and diffuse reflectance spectroscopy (DRS) before, during, and after the injection of local anesthesia containing epinephrine in human volunteers. We demonstrate the need to account for multiple absorbing species when applying linear spectral unmixing in order to obtain more clinically relevant sO2 values. In particular, we demonstrate how the inclusion of water absorption greatly affects the rate at which sO2 seemingly drops, which in turn sheds light on the current debate regarding the time required for local anesthesia with epinephrine to have an effect. In general, this work provides important insight into how spectral analysis methods need to be adapted to specific clinical scenarios to more accurately assess sO2. (Less)
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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Absorption spectroscopy, Clinical applications, Diffuse optical spectroscopy, Diffuse reflectance, Hyperspectral imaging, Spectral linewidth
in
Biomed. Opt. Express
volume
15
issue
3
pages
1995 - 2013
publisher
Optical Society of America
external identifiers
  • scopus:85186660037
  • pmid:38495727
ISSN
2156-7085
DOI
10.1364/BOE.506492
language
English
LU publication?
yes
id
5f860672-be91-4164-b2b3-bfc8f17164c6
date added to LUP
2024-03-11 10:08:17
date last changed
2024-12-04 14:12:36
@article{5f860672-be91-4164-b2b3-bfc8f17164c6,
  abstract     = {{Clinical determination of oxygen saturation (sO2) in patients is commonly performed via non-invasive optical techniques. However, reliance on a few wavelengths and some form of pre-determined calibration introduces limits to how these methods can be used. One example involves the assessment of sO2 after injection of local anesthetic using epinephrine, where some controversy exists around the time it takes for the epinephrine to have an effect. This is likely caused by a change in the tissue environment not accounted for by standard calibrated instruments and conventional analysis techniques. The present study aims to account for this changing environment by acquiring absorption spectra using hyperspectral imaging (HSI) and diffuse reflectance spectroscopy (DRS) before, during, and after the injection of local anesthesia containing epinephrine in human volunteers. We demonstrate the need to account for multiple absorbing species when applying linear spectral unmixing in order to obtain more clinically relevant sO2 values. In particular, we demonstrate how the inclusion of water absorption greatly affects the rate at which sO2 seemingly drops, which in turn sheds light on the current debate regarding the time required for local anesthesia with epinephrine to have an effect. In general, this work provides important insight into how spectral analysis methods need to be adapted to specific clinical scenarios to more accurately assess sO2.}},
  author       = {{Gustafsson, Nils and Bunke, Josefine and Magnusson, Ludvig and Albinsson, John and Hérnandez-Palacios, Julio and Sheikh, Rafi and Malmsjö, Malin and Merdasa, Aboma}},
  issn         = {{2156-7085}},
  keywords     = {{Absorption spectroscopy; Clinical applications; Diffuse optical spectroscopy; Diffuse reflectance; Hyperspectral imaging; Spectral linewidth}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{3}},
  pages        = {{1995--2013}},
  publisher    = {{Optical Society of America}},
  series       = {{Biomed. Opt. Express}},
  title        = {{Optimizing clinical O2 saturation mapping using hyperspectral imaging and diffuse reflectance spectroscopy in the context of epinephrine injection}},
  url          = {{http://dx.doi.org/10.1364/BOE.506492}},
  doi          = {{10.1364/BOE.506492}},
  volume       = {{15}},
  year         = {{2024}},
}