The MAP kinase substrate MKS1 is a regulator of plant defence responces
(2005) In EMBO Journal 24(14). p.2579-2589- Abstract
- Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the... (More)
- Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the WRKY transcription factors WRKY25 and WRKY33. WRKY25 and WRKY33 were shown to be in vitro substrates of MPK4, and a wrky33 knockout mutant was found to exhibit increased expression of the SA-related defense gene PR1. MKS1 may therefore contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors. (Less)
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https://lup.lub.lu.se/record/639690
- author
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- protein kinase, phosphoserine, disease resistance
- in
- EMBO Journal
- volume
- 24
- issue
- 14
- pages
- 2579 - 2589
- publisher
- Oxford University Press
- external identifiers
-
- scopus:23044508877
- pmid:15990873
- ISSN
- 1460-2075
- DOI
- 10.1038/sj.emboj.7600737
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biology building (Closed 2011) (011008000)
- id
- 43fb4b47-b4dd-41a4-9ff6-f3e6e918031e (old id 639690)
- date added to LUP
- 2016-04-01 11:56:35
- date last changed
- 2022-04-28 22:12:13
@article{43fb4b47-b4dd-41a4-9ff6-f3e6e918031e, abstract = {{Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the WRKY transcription factors WRKY25 and WRKY33. WRKY25 and WRKY33 were shown to be in vitro substrates of MPK4, and a wrky33 knockout mutant was found to exhibit increased expression of the SA-related defense gene PR1. MKS1 may therefore contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors.}}, author = {{Andreasson, Erik and Jenkins, Thomas and Brodersen, Peter and Thorgrimsen, Stephan and Petersen, Nikolaj HT and Zhu, Shijiang and Qiu, Jin-Long and Qiu, Jin-Long and Micheelsen, Pernille and Rocher, Anne and Petersen, Morten and Newman, Mari-Anne and Nielsen, Henrik Bjørn and Hirt, Heribert and Somssich, Imre and Mattsson, Ole and Mundy, John}}, issn = {{1460-2075}}, keywords = {{protein kinase; phosphoserine; disease resistance}}, language = {{eng}}, number = {{14}}, pages = {{2579--2589}}, publisher = {{Oxford University Press}}, series = {{EMBO Journal}}, title = {{The MAP kinase substrate MKS1 is a regulator of plant defence responces}}, url = {{http://dx.doi.org/10.1038/sj.emboj.7600737}}, doi = {{10.1038/sj.emboj.7600737}}, volume = {{24}}, year = {{2005}}, }