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Ultrastructural immunolocalization of cartilage oligomeric matrix protein, thrombospondin-4, and collagen fibril size in rodent Achilles tendon in relation to exercise

Soedersten, Fredrik ; Ekman, Stina ; Niehoff, Anja ; Zaucke, Frank ; Heinegård, Dick LU and Hultenby, Kjell (2007) In Connective Tissue Research 48(5). p.254-262
Abstract
Fourteen 3-week-old Sprague-Dawley rats were housed in pairs in standard cages (5 controls) and in individual cages with a running wheel. Four of these rats had run 27-36 km/week (low training-LT) and 5 had run 56-92 km/week (high training-HT). After 4 weeks, the rats were euthanized and Achilles tendons were fixed for electron microscopy. The ultrastructural distribution of cartilage oligomeric matrix protein (COMP) and thrombospondin (TSP)-4 and collagen fibril thickness in two different extracellular compartments were studied. The immunolabeling of COMP decreased with longer running distance and was significantly lower in both the pericellular (p = 0.009) and interterritorial (p = 0.03) compartments of the HT rats compared with the... (More)
Fourteen 3-week-old Sprague-Dawley rats were housed in pairs in standard cages (5 controls) and in individual cages with a running wheel. Four of these rats had run 27-36 km/week (low training-LT) and 5 had run 56-92 km/week (high training-HT). After 4 weeks, the rats were euthanized and Achilles tendons were fixed for electron microscopy. The ultrastructural distribution of cartilage oligomeric matrix protein (COMP) and thrombospondin (TSP)-4 and collagen fibril thickness in two different extracellular compartments were studied. The immunolabeling of COMP decreased with longer running distance and was significantly lower in both the pericellular (p = 0.009) and interterritorial (p = 0.03) compartments of the HT rats compared with the controls. TSP-4 immunolabeling was higher in the pericellular compared with the interterritorial compartments in all rats (p = 0.013) but was not correlated with COMP immunolabeling. No alterations in collagen fibril size were found in relation to running; however, the gold markers representing COMP and TSP-4 were mostly found at the dark bands, representing the gap region of the fibril. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
TSP-4, tendon, rodent, COMP, exercise
in
Connective Tissue Research
volume
48
issue
5
pages
254 - 262
publisher
Taylor & Francis
external identifiers
  • wos:000249539800005
  • scopus:34648843343
ISSN
1607-8438
DOI
10.1080/03008200701587505
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Connective Tissue Biology (013230151)
id
6ae503e4-0193-444b-933c-4b6d5724d69e (old id 656676)
date added to LUP
2016-04-01 11:59:31
date last changed
2022-01-26 21:11:49
@article{6ae503e4-0193-444b-933c-4b6d5724d69e,
  abstract     = {{Fourteen 3-week-old Sprague-Dawley rats were housed in pairs in standard cages (5 controls) and in individual cages with a running wheel. Four of these rats had run 27-36 km/week (low training-LT) and 5 had run 56-92 km/week (high training-HT). After 4 weeks, the rats were euthanized and Achilles tendons were fixed for electron microscopy. The ultrastructural distribution of cartilage oligomeric matrix protein (COMP) and thrombospondin (TSP)-4 and collagen fibril thickness in two different extracellular compartments were studied. The immunolabeling of COMP decreased with longer running distance and was significantly lower in both the pericellular (p = 0.009) and interterritorial (p = 0.03) compartments of the HT rats compared with the controls. TSP-4 immunolabeling was higher in the pericellular compared with the interterritorial compartments in all rats (p = 0.013) but was not correlated with COMP immunolabeling. No alterations in collagen fibril size were found in relation to running; however, the gold markers representing COMP and TSP-4 were mostly found at the dark bands, representing the gap region of the fibril.}},
  author       = {{Soedersten, Fredrik and Ekman, Stina and Niehoff, Anja and Zaucke, Frank and Heinegård, Dick and Hultenby, Kjell}},
  issn         = {{1607-8438}},
  keywords     = {{TSP-4; tendon; rodent; COMP; exercise}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{254--262}},
  publisher    = {{Taylor & Francis}},
  series       = {{Connective Tissue Research}},
  title        = {{Ultrastructural immunolocalization of cartilage oligomeric matrix protein, thrombospondin-4, and collagen fibril size in rodent Achilles tendon in relation to exercise}},
  url          = {{http://dx.doi.org/10.1080/03008200701587505}},
  doi          = {{10.1080/03008200701587505}},
  volume       = {{48}},
  year         = {{2007}},
}