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Mid-Infrared Photothermal Mesoscopy with Millimeter Field of View and Sub-micron Spatial Resolution

Tang, Rong ; Yin, Jiaze ; Weinberg, Bethany ; Lin, Haonan ; Lu, Jiahui ; Gate, David ; Klementieva, Oxana LU orcid and Cheng, Ji Xin (2026) Advanced Chemical Microscopy for Life Science and Translational Medicine 2026 In Progress in Biomedical Optics and Imaging - Proceedings of SPIE 13864.
Abstract

By optically sensing mid-infrared absorption through a visible probe beam, mid-infrared photothermal (MIP) microscopy has emerged as a powerful tool for chemical imaging with micromolar sensitivity and submicron spatial resolution. The adoption of spatially multiplexed camera-based widefield detection further enhanced the imaging speed. However, current widefield MIP systems suffer from a small field of view (FOV)—typically tens of square micrometers, which constrains their utility in large-area tissue imaging applications. Here, we report a laser-scan MIP mesoscope that achieves millimeter-scale FOV while preserving submicron resolution. By leveraging an all-reflective laser scanning architecture, low-magnification and medium... (More)

By optically sensing mid-infrared absorption through a visible probe beam, mid-infrared photothermal (MIP) microscopy has emerged as a powerful tool for chemical imaging with micromolar sensitivity and submicron spatial resolution. The adoption of spatially multiplexed camera-based widefield detection further enhanced the imaging speed. However, current widefield MIP systems suffer from a small field of view (FOV)—typically tens of square micrometers, which constrains their utility in large-area tissue imaging applications. Here, we report a laser-scan MIP mesoscope that achieves millimeter-scale FOV while preserving submicron resolution. By leveraging an all-reflective laser scanning architecture, low-magnification and medium numerical-aperture objectives, and a defocused signal collection scheme, our MIP mesoscope achieves a 1.2 × 1.2 mm2 FOV, 650 nm lateral resolution, and microsecond-scale pixel dwell time. In vivo chemical imaging of whole Caenorhabditis elegans and high-throughput detection of beta-amyloids in both mouse and human brain tissues associated with Alzheimer’s disease are demonstrated.

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Please use this url to cite or link to this publication:
author
; ; ; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Advanced Chemical Microscopy for Life Science and Translational Medicine 2026
series title
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
editor
Cheng, Ji-Xin ; Min, Wei and Simpson, Garth J.
volume
13864
article number
1386405
publisher
SPIE
conference name
Advanced Chemical Microscopy for Life Science and Translational Medicine 2026
conference location
San Francisco, United States
conference dates
2026-01-17 - 2026-01-19
external identifiers
  • scopus:105035719034
ISSN
1605-7422
2410-9045
ISBN
9781510696419
DOI
10.1117/12.3079290
language
English
LU publication?
yes
id
659efc80-3137-4b8c-98f0-a67f8ca6f97b
date added to LUP
2026-06-25 15:30:43
date last changed
2026-06-25 15:30:54
@inproceedings{659efc80-3137-4b8c-98f0-a67f8ca6f97b,
  abstract     = {{<p>By optically sensing mid-infrared absorption through a visible probe beam, mid-infrared photothermal (MIP) microscopy has emerged as a powerful tool for chemical imaging with micromolar sensitivity and submicron spatial resolution. The adoption of spatially multiplexed camera-based widefield detection further enhanced the imaging speed. However, current widefield MIP systems suffer from a small field of view (FOV)—typically tens of square micrometers, which constrains their utility in large-area tissue imaging applications. Here, we report a laser-scan MIP mesoscope that achieves millimeter-scale FOV while preserving submicron resolution. By leveraging an all-reflective laser scanning architecture, low-magnification and medium numerical-aperture objectives, and a defocused signal collection scheme, our MIP mesoscope achieves a 1.2 × 1.2 mm<sup>2</sup> FOV, 650 nm lateral resolution, and microsecond-scale pixel dwell time. In vivo chemical imaging of whole Caenorhabditis elegans and high-throughput detection of beta-amyloids in both mouse and human brain tissues associated with Alzheimer’s disease are demonstrated.</p>}},
  author       = {{Tang, Rong and Yin, Jiaze and Weinberg, Bethany and Lin, Haonan and Lu, Jiahui and Gate, David and Klementieva, Oxana and Cheng, Ji Xin}},
  booktitle    = {{Advanced Chemical Microscopy for Life Science and Translational Medicine 2026}},
  editor       = {{Cheng, Ji-Xin and Min, Wei and Simpson, Garth J.}},
  isbn         = {{9781510696419}},
  issn         = {{1605-7422}},
  language     = {{eng}},
  publisher    = {{SPIE}},
  series       = {{Progress in Biomedical Optics and Imaging - Proceedings of SPIE}},
  title        = {{Mid-Infrared Photothermal Mesoscopy with Millimeter Field of View and Sub-micron Spatial Resolution}},
  url          = {{http://dx.doi.org/10.1117/12.3079290}},
  doi          = {{10.1117/12.3079290}},
  volume       = {{13864}},
  year         = {{2026}},
}