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Progastrin-releasing peptide and gastrin-releasing peptide receptor mRNA expression in non-tumor tissues of the human gastrointestinal tract

Monstein, Hans-Juerg ; Grahn, Niclas ; Truedsson, Mikael and Ohlsson, Bodil LU (2006) In World Journal of Gastroenterology 12(16). p.2574-2578
Abstract
AIM: To investigate the expression of gastrin-releasing peptide (GRP) and GRP-receptor mRNA in non-tumor tissues of the human esophagus, gastrointestinal tract, pancreas and gallbladder using molecular biology techniques. METHODS: Poly A(+) mRNA was isolated from total RNA extracts using an automated nucleic acid extractor and, subsequently, converted into single-stranded cDNA (ss-cDNA). PCR amplifications were carried out using gene-specific GRP and GRP-receptor primers. The specificity of the PCR amplicons was further confirmed by Southern blot analyses using gene-specific GRP and GRP-receptor hybridization probes. RESULTS: Expression of GRP and GRP-receptor mRNA was detected at various levels in nearly all segments of the non-tumor... (More)
AIM: To investigate the expression of gastrin-releasing peptide (GRP) and GRP-receptor mRNA in non-tumor tissues of the human esophagus, gastrointestinal tract, pancreas and gallbladder using molecular biology techniques. METHODS: Poly A(+) mRNA was isolated from total RNA extracts using an automated nucleic acid extractor and, subsequently, converted into single-stranded cDNA (ss-cDNA). PCR amplifications were carried out using gene-specific GRP and GRP-receptor primers. The specificity of the PCR amplicons was further confirmed by Southern blot analyses using gene-specific GRP and GRP-receptor hybridization probes. RESULTS: Expression of GRP and GRP-receptor mRNA was detected at various levels in nearly all segments of the non-tumor specimens analysed, except the gallbladder. In most of the biopsy specimens, co-expression of both GRP and GRP-receptor mRNA appeared to take place. However, expression of GRP mRNA was more prominent than was GRP-receptor mRNA. CONCLUSION: GRP and GRP-receptor mRNAs are expressed throughout the gastrointestinal tract and provides information for the future mapping and determination of its physiological importance in normal and tumor cells. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
mRNA expression, (GRPR), gastrin releasing peptide (GRP), gastrin-releasing peptide receptor, morphogenesis, gastrointestinal tract
in
World Journal of Gastroenterology
volume
12
issue
16
pages
2574 - 2578
publisher
WJG Press
external identifiers
  • wos:000239996500014
  • pmid:16688804
  • scopus:33646446614
ISSN
1007-9327
language
English
LU publication?
yes
id
cca7df20-e078-4210-8caf-cafce0a1e276 (old id 686244)
date added to LUP
2016-04-01 12:00:44
date last changed
2022-01-26 21:33:58
@article{cca7df20-e078-4210-8caf-cafce0a1e276,
  abstract     = {{AIM: To investigate the expression of gastrin-releasing peptide (GRP) and GRP-receptor mRNA in non-tumor tissues of the human esophagus, gastrointestinal tract, pancreas and gallbladder using molecular biology techniques. METHODS: Poly A(+) mRNA was isolated from total RNA extracts using an automated nucleic acid extractor and, subsequently, converted into single-stranded cDNA (ss-cDNA). PCR amplifications were carried out using gene-specific GRP and GRP-receptor primers. The specificity of the PCR amplicons was further confirmed by Southern blot analyses using gene-specific GRP and GRP-receptor hybridization probes. RESULTS: Expression of GRP and GRP-receptor mRNA was detected at various levels in nearly all segments of the non-tumor specimens analysed, except the gallbladder. In most of the biopsy specimens, co-expression of both GRP and GRP-receptor mRNA appeared to take place. However, expression of GRP mRNA was more prominent than was GRP-receptor mRNA. CONCLUSION: GRP and GRP-receptor mRNAs are expressed throughout the gastrointestinal tract and provides information for the future mapping and determination of its physiological importance in normal and tumor cells.}},
  author       = {{Monstein, Hans-Juerg and Grahn, Niclas and Truedsson, Mikael and Ohlsson, Bodil}},
  issn         = {{1007-9327}},
  keywords     = {{mRNA expression; (GRPR); gastrin releasing peptide (GRP); gastrin-releasing peptide receptor; morphogenesis; gastrointestinal tract}},
  language     = {{eng}},
  number       = {{16}},
  pages        = {{2574--2578}},
  publisher    = {{WJG Press}},
  series       = {{World Journal of Gastroenterology}},
  title        = {{Progastrin-releasing peptide and gastrin-releasing peptide receptor mRNA expression in non-tumor tissues of the human gastrointestinal tract}},
  volume       = {{12}},
  year         = {{2006}},
}