Design of recombinant antibody microarrays for serum protein profiling: Targeting of complement proteins
(2007) In Journal of Proteome Research 6(9). p.3527-3536- Abstract
- Antibody-based microarrays is a novel technology with great promise for high-throughput proteomics. The process of designing high-performing arrays has, however, turned out to be challenging. Here, we have designed the next generation of a human recombinant scFv antibody microarray platform for protein expression profiling of nonfractionated biotinylated human plasma and serum proteomes. The setup, based on black polymer Maxisorb slides interfaced with a fluorescent-based read-out system, was found to provide specific, sensitive (subpicomolar (pM) range) and reproducible means for protein profiling. Further, a chip-to-chip normalization protocol critical for comparing data generated on different chips was devised. Finally, the microarray... (More)
- Antibody-based microarrays is a novel technology with great promise for high-throughput proteomics. The process of designing high-performing arrays has, however, turned out to be challenging. Here, we have designed the next generation of a human recombinant scFv antibody microarray platform for protein expression profiling of nonfractionated biotinylated human plasma and serum proteomes. The setup, based on black polymer Maxisorb slides interfaced with a fluorescent-based read-out system, was found to provide specific, sensitive (subpicomolar (pM) range) and reproducible means for protein profiling. Further, a chip-to-chip normalization protocol critical for comparing data generated on different chips was devised. Finally, the microarray data were found to correlate well with clinical laboratory data obtained using conventional methods, as demonstrated for a set of medium abundant (micromolar (mu M) to nanomolar (nM) range) protein analytes in serum and plasma samples derived from healthy and complement-deficient individuals. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/687896
- author
- Ingvarsson, Johan LU ; Larsson, Anette ; Sjöholm, Anders LU ; Truedsson, Lennart LU ; Jansson, Bo LU ; Borrebaeck, Carl LU and Wingren, Christer LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- profiling, protein expression, recombinant antibody microarrays, serum proteomics, complement proteins
- in
- Journal of Proteome Research
- volume
- 6
- issue
- 9
- pages
- 3527 - 3536
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000249371000020
- scopus:34948911792
- pmid:17696517
- ISSN
- 1535-3893
- DOI
- 10.1021/pr070204f
- language
- English
- LU publication?
- yes
- id
- 41f28b4c-4f5c-4ba5-9d55-146223f3a768 (old id 687896)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17696517&dopt=Abstract
- date added to LUP
- 2016-04-01 12:00:11
- date last changed
- 2022-02-18 08:25:00
@article{41f28b4c-4f5c-4ba5-9d55-146223f3a768,
abstract = {{Antibody-based microarrays is a novel technology with great promise for high-throughput proteomics. The process of designing high-performing arrays has, however, turned out to be challenging. Here, we have designed the next generation of a human recombinant scFv antibody microarray platform for protein expression profiling of nonfractionated biotinylated human plasma and serum proteomes. The setup, based on black polymer Maxisorb slides interfaced with a fluorescent-based read-out system, was found to provide specific, sensitive (subpicomolar (pM) range) and reproducible means for protein profiling. Further, a chip-to-chip normalization protocol critical for comparing data generated on different chips was devised. Finally, the microarray data were found to correlate well with clinical laboratory data obtained using conventional methods, as demonstrated for a set of medium abundant (micromolar (mu M) to nanomolar (nM) range) protein analytes in serum and plasma samples derived from healthy and complement-deficient individuals.}},
author = {{Ingvarsson, Johan and Larsson, Anette and Sjöholm, Anders and Truedsson, Lennart and Jansson, Bo and Borrebaeck, Carl and Wingren, Christer}},
issn = {{1535-3893}},
keywords = {{profiling; protein expression; recombinant antibody microarrays; serum proteomics; complement proteins}},
language = {{eng}},
number = {{9}},
pages = {{3527--3536}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Journal of Proteome Research}},
title = {{Design of recombinant antibody microarrays for serum protein profiling: Targeting of complement proteins}},
url = {{http://dx.doi.org/10.1021/pr070204f}},
doi = {{10.1021/pr070204f}},
volume = {{6}},
year = {{2007}},
}